Cloned (Comment) | Organism |
---|---|
cloned into pMAL-c4X vector and expressed in Escherichia coli Origami B (DE3) cells as an N-maltose binding protein (MBP)-tagged and C-His6-tagged fusion protein | Helicobacter pylori |
NmLgtB is cloned into pET15b vector and expressed in Escherichia coli BL21 (DE3) cells as N-terminal His6-tagged fusion protein. Under common expression conditions only a low amount of soluble active enzyme is obtained. The expression level is improved 15fold to 12.5 mg/L culture under osmotic stress condition using LB medium supplemented with 0.2% glucose and a higher concentration of NaCl | Neisseria meningitidis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Helicobacter pylori | - |
- |
- |
Neisseria meningitidis | - |
serogroup B strain | - |
Storage Stability | Organism |
---|---|
-20°C, 10% glycerol, up to 14 months, no loss of activity | Neisseria meningitidis |
-20°C, 10% glycerol, up to 14 months, no loss of activity | Helicobacter pylori |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-methylumbelliferyl-beta-D-GlcNAc + UDP-galactose | Hp14GalT specifically recognizes GlcNAc-terminated glycans as acceptors | Helicobacter pylori | ? | - |
? | |
additional information | Hp1-4GalT can not use Glc, alpha-linked GlcNAc, and N-modified GlcNAc as acceptors but it can use 6-O-sulfated GlcNAc such as GlcNAc6S and GlcNAc6SbetaProN3 as suitable acceptor substrates | Helicobacter pylori | ? | - |
? | |
additional information | NmLgtB can use Glc, both alpha- and beta-linked GlcNAc, and N-modified but not 6-O-sulfated GlcNAc as acceptors | Neisseria meningitidis | ? | - |
? | |
UDP-alpha-D-galactose + 4-methylumbelliferyl beta-D-glucopyranoside | - |
Neisseria meningitidis | UDP + 4-methylumbelliferyl 4-O-beta-D-galactopyranosyl-beta-D-glucopyranoside | - |
? | |
UDP-galactose + 4-methylumbelliferyl N-acetyl-beta-D-glucosamine | NmLgtB can use both GlcNAc- and Glc-terminated glycans as acceptor substrates | Neisseria meningitidis | UDP + 4-methylumbelliferyl 4-O-beta-D-galactopyranosyl-N-acetyl-beta-D-glucosaminopyranoside | - |
? |
Synonyms | Comment | Organism |
---|---|---|
beta1-4-galactosyltransferase | - |
Neisseria meningitidis |
beta1-4-galactosyltransferase | - |
Helicobacter pylori |
beta1-4GalT | - |
Neisseria meningitidis |
beta1-4GalT | - |
Helicobacter pylori |
Hp1-4GalT | - |
Helicobacter pylori |
NmLgtB | - |
Neisseria meningitidis |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Neisseria meningitidis |
37 | - |
assay at | Helicobacter pylori |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
assay at | Neisseria meningitidis |
7.5 | - |
assay at | Helicobacter pylori |