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Literature summary for 2.4.1.9 extracted from
- Enzymatic synthesis and characterization of fructooligosaccharides and novel maltosylfructosides by inulosucrase from Lactobacillus gasseri DSM 20604 (2013), Appl. Environ. Microbiol., 79, 4129-4140.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| gene inuGB, recombinant expression of His6-tagged truncated enzyme mutant lacking the cell-anchoring motif in Escherichia coli strain DH10B | Lactobacillus gasseri |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| additional information | construction of an enzyme mutant lacking the cell-anchoring motif | Lactobacillus gasseri |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Lactobacillus gasseri | D3WYV9 | gene inuGB | - |
| Lactobacillus gasseri DSM 20604 | D3WYV9 | gene inuGB | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant His6-tagged truncated enzyme mutant from Escherichia coli strain DH10B by nickel affinity chromatography | Lactobacillus gasseri |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 10.5 | - |
purified recombinant truncated His-tagged enzyme, pH 5.2, 55°C | Lactobacillus gasseri |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | enzymatic synthesis of maltosylfructosides derived from sucrose-maltose mixtures by the enzyme from strain DSM 20604, structure analysis, and relation to fructooligosaccharides contents, overview | Lactobacillus gasseri | ? | - |
? |  |
| additional information | enzymatic synthesis of maltosylfructosides derived from sucrose-maltose mixtures by the enzyme from strain DSM 20604, structure analysis, and relation to fructooligosaccharides contents, overview | Lactobacillus gasseri DSM 20604 | ? | - |
? | |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 55 | - |
recombinant enzyme | Lactobacillus gasseri |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 5.2 | - |
recombinant enzyme | Lactobacillus gasseri |
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Release 2023.1 (January 2023)
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