Literature summary for 2.4.1.9 extracted from

Van Hijum, S.A.F.T.M.; van Geel-Schutten, G.H.; Rahaoui, H.; van der Maarel, M.J.E.C.; Dijkhuizen, L.
Characterization of a novel fructosyltransferase from Lactobacillus reuteri that synthesizes high molecular weight inulin and inulin oligosaccharides (2002), Appl. Environ. Microbiol., 68, 4390-4398.

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Cloned(Commentary)

Cloned (Comment)	Organism
expression of the full-length protein and a truncated protein with a 100 amino acid truncation at the C-terminus from amino acid 699 onwards, with a C-terminal His-tag, InuDELTA699His	Limosilactobacillus reuteri

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
sucrose + [(beta-D-Fruf-(2-1))n]-alpha-D-Glup	Limosilactobacillus reuteri	-	alpha-D-glucose + [(beta-D-Fruf-(2-1))n+1]-alpha-D-Glup	-	?

Organism

Organism	UniProt	Comment	Textmining
Limosilactobacillus reuteri	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
recombinant enzyme	Limosilactobacillus reuteri

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
103.7	-	-	Limosilactobacillus reuteri

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
sucrose + [(beta-D-Fruf-(2-1))n]-alpha-D-Glup	-	Limosilactobacillus reuteri	alpha-D-glucose + [(beta-D-Fruf-(2-1))n+1]-alpha-D-Glup	-	?
sucrose + [(beta-D-Fruf-(2-1))n]-alpha-D-Glup	the recombinant truncated enzyme produces both the fructooligosaccharides 1-kestose and nystose and high-molecular-weight-fructan with beta-(2, 1) linkages. The wild-type enzyme only produces 1-kestose, 95%, and nystose, 5%	Limosilactobacillus reuteri	alpha-D-glucose + [(beta-D-Fruf-(2-1))n+1]-alpha-D-Glup	-	?

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Release 2023.1 (January 2023)