BRENDA - Enzyme Database
show all sequences of 2.4.1.88

Solubilization and partial characterization of UDP-N-acetylgalactosamine: globoside alpha-N-acetylgalactosaminyltransferase from dog spleen microsomes

Ishibashi, T.; Ohkubo, I.; Makita, A.; Biochim. Biophys. Acta 484, 24-34 (1977)

Data extracted from this reference:

Activating Compound
Activating Compound
Commentary
Organism
Structure
diphosphatidylglycerol
activation
Canis lupus familiaris
GSH
slight stimulation
Canis lupus familiaris
additional information
requirement for phospholipids
Canis lupus familiaris
phosphatidic acid
activation
Canis lupus familiaris
phosphatidylcholine
activation
Canis lupus familiaris
phosphatidylethanolamine
activation
Canis lupus familiaris
phosphatidylinositol
activation
Canis lupus familiaris
phosphatidylserine
activation
Canis lupus familiaris
sphingomyelin
activation
Canis lupus familiaris
Triton X-100
activation; best at 0.3-0.6%
Canis lupus familiaris
General Stability
General Stability
Organism
phosphatidylserine stabilizes
Canis lupus familiaris
Inhibitors
Inhibitors
Commentary
Organism
Structure
ADP
-
Canis lupus familiaris
ATP
-
Canis lupus familiaris
CDP
-
Canis lupus familiaris
EDTA
strong
Canis lupus familiaris
GDP
-
Canis lupus familiaris
IDP
-
Canis lupus familiaris
additional information
no inhibitor: NaF
Canis lupus familiaris
NaN3
weak
Canis lupus familiaris
PCMB
over 90% inhibition at 2 mM
Canis lupus familiaris
UDP
85% inhibition at 1.5 mM
Canis lupus familiaris
uridine
weak
Canis lupus familiaris
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.01
-
UDP-N-acetylgalactosamine
-
Canis lupus familiaris
0.5
-
globoside
-
Canis lupus familiaris
Localization
Localization
Commentary
Organism
GeneOntology No.
Textmining
membrane
-
Canis lupus familiaris
16020
-
microsome
-
Canis lupus familiaris
-
-
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
Mg2+
no effect
Canis lupus familiaris
Mn2+
absolute requirement, cannot be replaced by Ca2+ and Mg2+; optimal concentration: 10 mM
Canis lupus familiaris
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
UDP-N-acetyl-D-galactosamine + N-acetyl-D-galactosaminyl-1,3-D-galactosyl-1,4-D-galactosyl-1,4-D-glucosylceramide
Canis lupus familiaris
involved in biosynthesis of carbohydrate moiety of sphingoglycolipids
UDP + N-acetyl-D-galactosaminyl-1,3-N-acetyl-D-galactosaminyl-1,3-D-galactosyl-1,4-D-galactosyl-1,4-D-glucosylceramide
i.e. Forssman hapten
Canis lupus familiaris
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Canis lupus familiaris
-
mongrel dog, adult
-
Purification (Commentary)
Commentary
Organism
partial
Canis lupus familiaris
Source Tissue
Source Tissue
Commentary
Organism
Textmining
spleen
-
Canis lupus familiaris
-
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
additional information
-
-
Canis lupus familiaris
Storage Stability
Storage Stability
Organism
-20°C, at least 2 weeks without loss of activity
Canis lupus familiaris
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
UDP-N-acetyl-D-galactosamine + N-acetyl-D-galactosaminyl-1,3-D-galactosyl-1,4-D-galactosyl-1,4-D-glucosylceramide
-
489482
Canis lupus familiaris
UDP + N-acetyl-D-galactosaminyl-1,3-N-acetyl-D-galactosaminyl-1,3-D-galactosyl-1,4-D-galactosyl-1,4-D-glucosylceramide
i.e. Forssman hapten
489482
Canis lupus familiaris
?
UDP-N-acetyl-D-galactosamine + N-acetyl-D-galactosaminyl-1,3-D-galactosyl-1,4-D-galactosyl-1,4-D-glucosylceramide
involved in biosynthesis of carbohydrate moiety of sphingoglycolipids
489482
Canis lupus familiaris
UDP + N-acetyl-D-galactosaminyl-1,3-N-acetyl-D-galactosaminyl-1,3-D-galactosyl-1,4-D-galactosyl-1,4-D-glucosylceramide
i.e. Forssman hapten
489482
Canis lupus familiaris
?
Temperature Optimum [°C]
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
37
-
assay at
Canis lupus familiaris
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
6.6
-
-
Canis lupus familiaris
Activating Compound (protein specific)
Activating Compound
Commentary
Organism
Structure
diphosphatidylglycerol
activation
Canis lupus familiaris
GSH
slight stimulation
Canis lupus familiaris
additional information
requirement for phospholipids
Canis lupus familiaris
phosphatidic acid
activation
Canis lupus familiaris
phosphatidylcholine
activation
Canis lupus familiaris
phosphatidylethanolamine
activation
Canis lupus familiaris
phosphatidylinositol
activation
Canis lupus familiaris
phosphatidylserine
activation
Canis lupus familiaris
sphingomyelin
activation
Canis lupus familiaris
Triton X-100
activation; best at 0.3-0.6%
Canis lupus familiaris
General Stability (protein specific)
General Stability
Organism
phosphatidylserine stabilizes
Canis lupus familiaris
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
ADP
-
Canis lupus familiaris
ATP
-
Canis lupus familiaris
CDP
-
Canis lupus familiaris
EDTA
strong
Canis lupus familiaris
GDP
-
Canis lupus familiaris
IDP
-
Canis lupus familiaris
additional information
no inhibitor: NaF
Canis lupus familiaris
NaN3
weak
Canis lupus familiaris
PCMB
over 90% inhibition at 2 mM
Canis lupus familiaris
UDP
85% inhibition at 1.5 mM
Canis lupus familiaris
uridine
weak
Canis lupus familiaris
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.01
-
UDP-N-acetylgalactosamine
-
Canis lupus familiaris
0.5
-
globoside
-
Canis lupus familiaris
Localization (protein specific)
Localization
Commentary
Organism
GeneOntology No.
Textmining
membrane
-
Canis lupus familiaris
16020
-
microsome
-
Canis lupus familiaris
-
-
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
Mg2+
no effect
Canis lupus familiaris
Mn2+
absolute requirement, cannot be replaced by Ca2+ and Mg2+; optimal concentration: 10 mM
Canis lupus familiaris
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
UDP-N-acetyl-D-galactosamine + N-acetyl-D-galactosaminyl-1,3-D-galactosyl-1,4-D-galactosyl-1,4-D-glucosylceramide
Canis lupus familiaris
involved in biosynthesis of carbohydrate moiety of sphingoglycolipids
UDP + N-acetyl-D-galactosaminyl-1,3-N-acetyl-D-galactosaminyl-1,3-D-galactosyl-1,4-D-galactosyl-1,4-D-glucosylceramide
i.e. Forssman hapten
Canis lupus familiaris
?
Purification (Commentary) (protein specific)
Commentary
Organism
partial
Canis lupus familiaris
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
spleen
-
Canis lupus familiaris
-
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
additional information
-
-
Canis lupus familiaris
Storage Stability (protein specific)
Storage Stability
Organism
-20°C, at least 2 weeks without loss of activity
Canis lupus familiaris
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
UDP-N-acetyl-D-galactosamine + N-acetyl-D-galactosaminyl-1,3-D-galactosyl-1,4-D-galactosyl-1,4-D-glucosylceramide
-
489482
Canis lupus familiaris
UDP + N-acetyl-D-galactosaminyl-1,3-N-acetyl-D-galactosaminyl-1,3-D-galactosyl-1,4-D-galactosyl-1,4-D-glucosylceramide
i.e. Forssman hapten
489482
Canis lupus familiaris
?
UDP-N-acetyl-D-galactosamine + N-acetyl-D-galactosaminyl-1,3-D-galactosyl-1,4-D-galactosyl-1,4-D-glucosylceramide
involved in biosynthesis of carbohydrate moiety of sphingoglycolipids
489482
Canis lupus familiaris
UDP + N-acetyl-D-galactosaminyl-1,3-N-acetyl-D-galactosaminyl-1,3-D-galactosyl-1,4-D-galactosyl-1,4-D-glucosylceramide
i.e. Forssman hapten
489482
Canis lupus familiaris
?
Temperature Optimum [°C] (protein specific)
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
37
-
assay at
Canis lupus familiaris
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
6.6
-
-
Canis lupus familiaris
Other publictions for EC 2.4.1.88
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
735858
Jacob
Expression of GBGT1 is epigene ...
Homo sapiens
BMC Mol. Biol.
15
24
2014
-
-
1
-
-
-
-
-
-
-
-
1
-
2
-
-
-
-
-
9
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
9
-
-
2
-
-
-
-
-
-
-
-
-
2
3
3
2
-
-
692523
Turcot-Dubois
Long-term evolution of the CAZ ...
Rattus norvegicus
Glycobiology
17
516-528
2007
-
-
1
-
-
-
-
-
-
-
-
-
-
13
-
-
-
-
-
4
1
-
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-
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2
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8
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
658838
Heissigerova
Molecular modeling of glycosyl ...
Canis lupus familiaris
Glycobiology
13
377-386
2003
-
-
-
1
-
-
-
-
-
1
-
1
-
1
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-
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1
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-
-
-
2
1
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1
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1
-
1
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-
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-
-
-
-
-
2
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
658898
Elliott
Forssman synthetase expression ...
Canis lupus familiaris, Mus musculus, no activity in Homo sapiens
Infect. Immun.
71
6543-6552
2003
-
-
2
-
-
-
-
-
-
-
-
2
-
7
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
489386
Okajima
Expression cloning of human gl ...
Homo sapiens
J. Biol. Chem.
275
40498-40503
2000
-
-
1
-
-
-
-
-
-
-
-
-
-
4
-
-
-
-
-
1
-
-
1
-
1
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
1
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
489485
Xu
Characterization of the human ...
Canis lupus familiaris, Homo sapiens
J. Biol. Chem.
274
29390-29398
1999
-
-
2
-
2
-
-
-
2
-
2
1
-
7
-
-
-
-
-
1
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
2
-
-
-
-
-
2
-
3
1
-
-
-
-
-
1
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
489484
Haslam
Expression cloning of Forssman ...
Canis lupus familiaris
Proc. Natl. Acad. Sci. USA
93
10697-10702
1996
-
-
1
-
-
-
-
-
-
-
1
-
-
1
-
-
-
-
-
1
-
-
1
-
1
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
-
1
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
489481
Taniguchi
UDP-N-acetylgalactosamine:glob ...
Canis lupus familiaris
J. Biol. Chem.
257
10631-10637
1982
-
-
-
-
-
3
5
2
2
7
3
1
-
1
-
-
1
-
-
1
1
2
6
1
1
-
-
-
1
1
-
-
-
-
-
-
-
-
-
-
-
3
-
5
-
2
2
7
3
1
-
-
-
1
-
1
1
2
6
1
1
-
-
-
1
1
-
-
-
-
-
-
-
-
489482
Ishibashi
Solubilization and partial cha ...
Canis lupus familiaris
Biochim. Biophys. Acta
484
24-34
1977
10
-
-
-
-
1
11
2
2
2
-
1
-
2
-
-
1
-
-
2
1
1
2
-
1
-
-
-
1
-
-
-
-
-
-
10
-
-
-
-
-
1
-
11
-
2
2
2
-
1
-
-
-
1
-
2
1
1
2
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
489483
Ishibashi
Effects of uridine nucleotides ...
Cavia porcellus
Biochim. Biophys. Acta
429
759-767
1976
1
-
-
-
-
-
7
-
1
-
-
1
-
1
-
-
-
-
-
1
-
-
1
-
1
-
-
-
1
-
-
-
1
-
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1
-
-
-
-
-
-
-
7
1
-
1
-
-
1
-
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-
-
1
-
-
1
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
489480
Kijimoto
Biosynthesis of Forssman hapte ...
Cavia porcellus
Biochem. Biophys. Res. Commun.
56
177-184
1974
3
-
-
-
-
-
11
2
1
2
-
1
-
1
-
-
-
-
-
5
1
-
1
-
1
-
-
-
1
-
-
-
-
-
-
3
-
-
-
-
-
-
-
11
-
2
1
2
-
1
-
-
-
-
-
5
1
-
1
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-