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Literature summary for 2.4.1.88 extracted from

  • Kijimoto, S.; Ishibashi, T.; Makita, A.
    Biosynthesis of Forssman hapten from globoside by alpha-N-acetylgalactosaminyltransferase of guinea pig tissues (1974), Biochem. Biophys. Res. Commun., 56, 177-184.
    View publication on PubMed
Search for more literature for 2.4.1.88

Activating Compound

Activating Compound Comment Organism Structure
ATP slightly activating Cavia porcellus
sodium taurocholate activation Cavia porcellus
Triton X-100 activation Cavia porcellus

Inhibitors

Inhibitors Comment Organism Structure
ATP no inhibition Cavia porcellus
EDTA
-
 Cavia porcellus
additional information no inhibitor: NaF; not affected by GSH, N-acetylgalactosamine, and glucuronolactone Cavia porcellus
NaN3
-
 Cavia porcellus
PCMB no inhibition Cavia porcellus
sodium deoxycholate
-
 Cavia porcellus
Triton CF-54
-
 Cavia porcellus
Tween 20
-
 Cavia porcellus
Tween 80
-
 Cavia porcellus
UDP-glucose strong Cavia porcellus
UDP-N-acetylglucosamine no inhibition Cavia porcellus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.1
-
 UDP-N-acetylgalactosamine
-
 Cavia porcellus
0.4
-
 globoside
-
 Cavia porcellus

Localization

Localization Comment Organism GeneOntology No. Textmining
microsome
-
 Cavia porcellus
-
-

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ no effect Cavia porcellus
Mn2+ requirement Cavia porcellus
Mn2+ optimal concentration: 10 mM Cavia porcellus
Mn2+ Mg2+ and Ca2+ can hardly replace Mn2+ Cavia porcellus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
UDP-N-acetyl-D-galactosamine + N-acetyl-D-galactosaminyl-1,3-D-galactosyl-1,4-D-galactosyl-1,4-D-glucosylceramide Cavia porcellus
-
 UDP + N-acetyl-D-galactosaminyl-1,3-N-acetyl-D-galactosaminyl-1,3-D-galactosyl-1,4-D-galactosyl-1,4-D-glucosylceramide i.e. Forssman hapten ?

Organism

Organism UniProt Comment Textmining
Cavia porcellus
-
-
-

Source Tissue

Source Tissue Comment Organism Textmining
brain low activity Cavia porcellus
-
kidney
-
 Cavia porcellus
-
liver low activity Cavia porcellus
-
small intestine
-
 Cavia porcellus
-
spleen
-
 Cavia porcellus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
 tissue distribution of enzyme activity Cavia porcellus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
UDP-N-acetyl-alpha-D-galactosamine + globoside
-
 Cavia porcellus UDP + N-acetyl-D-galactosaminyl-N-acetyl-D-galactosaminyl-1,3-D-galactosyl-1,4-D-galactosyl-1,4-D-glucosylceramide
-
 ?
UDP-N-acetyl-D-galactosamine + N-acetyl-D-galactosaminyl-1,3-D-galactosyl-1,4-D-galactosyl-1,4-D-glucosylceramide
-
 Cavia porcellus UDP + N-acetyl-D-galactosaminyl-1,3-N-acetyl-D-galactosaminyl-1,3-D-galactosyl-1,4-D-galactosyl-1,4-D-glucosylceramide i.e. Forssman hapten ?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Cavia porcellus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.7
-
-
 Cavia porcellus