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Literature summary for 2.4.1.82 extracted from

  • Lehle, L.; Tanner, W.
    The function of myo-inositol in the biosynthesis of raffinose. Purification and characterization of galactinol:sucrose 6-galactosyltransferase from Vicia faba seeds (1973), Eur. J. Biochem., 38, 103-110.
    View publication on PubMed

General Stability

General Stability Organism
dithioerythritol stabilizes during purification Vicia faba

Inhibitors

Inhibitors Comment Organism Structure
Ag+ 1 mM, strong Vicia faba
Al3+ 1 mM, strong Vicia faba
Hg2+ 1 mM, strong Vicia faba
iodoacetamide 49% inhibition at 1 mM Vicia faba
Mn2+
-
Vicia faba
N-ethylmaleimide 90% inhibition at 1 mM Vicia faba
Zn2+ 1 mM, strong Vicia faba

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1
-
sucrose cosubstrate galactinol Vicia faba
2.9
-
sucrose + raffinose, exchange reaction Vicia faba
7
-
galactinol + sucrose Vicia faba
10
-
raffinose + sucrose, exchange reaction Vicia faba

Localization

Localization Comment Organism GeneOntology No. Textmining
cytoplasm
-
Vicia faba 5737
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
80000
-
gel filtration Vicia faba
100000
-
glycerol density gradient centrifugation Vicia faba

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1-alpha-D-galactosyl-myo-inositol + sucrose Vicia faba first step in biosynthesis of raffinose sugars myo-inositol + raffinose
-
?

Organism

Organism UniProt Comment Textmining
Vicia faba
-
-
-

Purification (Commentary)

Purification (Comment) Organism
partial Vicia faba

Source Tissue

Source Tissue Comment Organism Textmining
seed
-
Vicia faba
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.0012
-
crude seed extract Vicia faba
0.497
-
purified enzyme Vicia faba

Storage Stability

Storage Stability Organism
4°C, in crude extract, t1/2: 3 days Vicia faba
frozen, purified enzyme, at least 1 month Vicia faba

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1-alpha-D-galactosyl-myo-inositol + H2O hydrolysis, in the absence of acceptor substrate Vicia faba myo-inositol + D-galactose
-
?
1-alpha-D-galactosyl-myo-inositol + sucrose
-
Vicia faba myo-inositol + raffinose
-
r
1-alpha-D-galactosyl-myo-inositol + sucrose first step in biosynthesis of raffinose sugars Vicia faba myo-inositol + raffinose
-
?
4-nitrophenol-alpha-D-galactopyranoside + sucrose
-
Vicia faba 4-nitrophenol + raffinose
-
?
additional information UDP-galactose is no substrate Vicia faba ?
-
?
raffinose + sucrose exchange reaction Vicia faba sucrose + raffinose
-
r

Synonyms

Synonyms Comment Organism
galactinol:sucrose 6-galactosyl transferase
-
Vicia faba

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
42
-
-
Vicia faba

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
28 50 about half-maximal activity at 28°C and 50°C Vicia faba

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
50
-
10 min, 80% loss of activity, galactinol and raffinose protect, not sucrose Vicia faba

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
-
Vicia faba

pH Range

pH Minimum pH Maximum Comment Organism
5.7 7.8 about half-maximal activity at pH 5.7 and pH 7.8 Vicia faba