General Stability | Organism |
---|---|
dithioerythritol stabilizes during purification | Vicia faba |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Ag+ | 1 mM, strong | Vicia faba | |
Al3+ | 1 mM, strong | Vicia faba | |
Hg2+ | 1 mM, strong | Vicia faba | |
iodoacetamide | 49% inhibition at 1 mM | Vicia faba | |
Mn2+ | - |
Vicia faba | |
N-ethylmaleimide | 90% inhibition at 1 mM | Vicia faba | |
Zn2+ | 1 mM, strong | Vicia faba |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1 | - |
sucrose | cosubstrate galactinol | Vicia faba | |
2.9 | - |
sucrose | + raffinose, exchange reaction | Vicia faba | |
7 | - |
galactinol | + sucrose | Vicia faba | |
10 | - |
raffinose | + sucrose, exchange reaction | Vicia faba |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytoplasm | - |
Vicia faba | 5737 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
80000 | - |
gel filtration | Vicia faba |
100000 | - |
glycerol density gradient centrifugation | Vicia faba |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
1-alpha-D-galactosyl-myo-inositol + sucrose | Vicia faba | first step in biosynthesis of raffinose sugars | myo-inositol + raffinose | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Vicia faba | - |
- |
- |
Purification (Comment) | Organism |
---|---|
partial | Vicia faba |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
seed | - |
Vicia faba | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.0012 | - |
crude seed extract | Vicia faba |
0.497 | - |
purified enzyme | Vicia faba |
Storage Stability | Organism |
---|---|
4°C, in crude extract, t1/2: 3 days | Vicia faba |
frozen, purified enzyme, at least 1 month | Vicia faba |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
1-alpha-D-galactosyl-myo-inositol + H2O | hydrolysis, in the absence of acceptor substrate | Vicia faba | myo-inositol + D-galactose | - |
? | |
1-alpha-D-galactosyl-myo-inositol + sucrose | - |
Vicia faba | myo-inositol + raffinose | - |
r | |
1-alpha-D-galactosyl-myo-inositol + sucrose | first step in biosynthesis of raffinose sugars | Vicia faba | myo-inositol + raffinose | - |
? | |
4-nitrophenol-alpha-D-galactopyranoside + sucrose | - |
Vicia faba | 4-nitrophenol + raffinose | - |
? | |
additional information | UDP-galactose is no substrate | Vicia faba | ? | - |
? | |
raffinose + sucrose | exchange reaction | Vicia faba | sucrose + raffinose | - |
r |
Synonyms | Comment | Organism |
---|---|---|
galactinol:sucrose 6-galactosyl transferase | - |
Vicia faba |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
42 | - |
- |
Vicia faba |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
28 | 50 | about half-maximal activity at 28°C and 50°C | Vicia faba |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
10 min, 80% loss of activity, galactinol and raffinose protect, not sucrose | Vicia faba |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
- |
Vicia faba |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
5.7 | 7.8 | about half-maximal activity at pH 5.7 and pH 7.8 | Vicia faba |