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Literature summary for 2.4.1.80 extracted from

  • Marks, D.L.; Wu, K.; Paul, P.; Kamisaka, Y.; Watanabe, R.; Pagano, R.E.
    Oligomerization and topology of the Golgi membrane protein glucosylceramide synthase (1999), J. Biol. Chem., 274, 451-456.
    View publication on PubMed

Protein Variants

Protein Variants Comment Organism
additional information deletion mutants, lacking the first 10 or the last 8 amino acid residues, show only 4% of the wild-type activity Rattus norvegicus

General Stability

General Stability Organism
labile enzyme Rattus norvegicus

Inhibitors

Inhibitors Comment Organism Structure
Trypsin
-
Rattus norvegicus

Localization

Localization Comment Organism GeneOntology No. Textmining
Golgi membrane
-
Rattus norvegicus 139
-
microsome
-
Homo sapiens
-
-
microsome associated with membranes, structural integrity of the microsomal membranes is essential for activity Rattus norvegicus
-
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
additional information
-
C- and N-terminal amino acid sequences Homo sapiens
additional information
-
C- and N-terminal amino acid sequences Rattus norvegicus
additional information
-
enzyme forms dimeric or oligomeric complexes with another protein in the Golgi membrane vivo, topology Homo sapiens
additional information
-
enzyme forms dimeric or oligomeric complexes with another protein in the Golgi membrane vivo, topology Rattus norvegicus
38000
-
x * 38000, SDS-PAGE Homo sapiens
38000
-
x * 38000, native and recombinant enzyme, SDS-PAGE Rattus norvegicus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
UDP-glucose + N-acylsphingosine Homo sapiens
-
UDP + D-glucosyl-N-acylsphingosine
-
?
UDP-glucose + N-acylsphingosine Rattus norvegicus initial key enzyme in glycosphingolipid biosynthesis UDP + D-glucosyl-N-acylsphingosine i.e. glucosylceramide ?

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-
Rattus norvegicus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
partial from Golgi membranes Rattus norvegicus

Source Tissue

Source Tissue Comment Organism Textmining
brain
-
Homo sapiens
-
brain
-
Rattus norvegicus
-
keratinocyte
-
Homo sapiens
-
liver
-
Rattus norvegicus
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
UDP-glucose + N-acylsphingosine
-
Homo sapiens UDP + D-glucosyl-N-acylsphingosine
-
?
UDP-glucose + N-acylsphingosine i.e. ceramide Homo sapiens UDP + D-glucosyl-N-acylsphingosine i.e. glucosylceramide ?
UDP-glucose + N-acylsphingosine i.e. ceramide Rattus norvegicus UDP + D-glucosyl-N-acylsphingosine i.e. glucosylceramide ?
UDP-glucose + N-acylsphingosine initial key enzyme in glycosphingolipid biosynthesis Rattus norvegicus UDP + D-glucosyl-N-acylsphingosine i.e. glucosylceramide ?
UDP-glucose + N-[7-(4-nitrobenzo-2-oxa-1,3-diazole)]-6-aminocaproyl-D-erythro-sphingosine synthetic fluorescent ceramide substrate analogue for fluorescence enzyme assay Rattus norvegicus UDP + D-glucosyl-N-[7-(4-nitrobenzo-2-oxa-1,3-diazole)]-6-aminocaproyl-D-erythro-sphingosine
-
?

Subunits

Subunits Comment Organism
? x * 38000, SDS-PAGE Homo sapiens
? x * 38000, native and recombinant enzyme, SDS-PAGE Rattus norvegicus

Synonyms

Synonyms Comment Organism
GCS
-
Homo sapiens
GCS
-
Rattus norvegicus