Protein Variants | Comment | Organism |
---|---|---|
additional information | deletion mutants, lacking the first 10 or the last 8 amino acid residues, show only 4% of the wild-type activity | Rattus norvegicus |
General Stability | Organism |
---|---|
labile enzyme | Rattus norvegicus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Trypsin | - |
Rattus norvegicus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
Golgi membrane | - |
Rattus norvegicus | 139 | - |
microsome | - |
Homo sapiens | - |
- |
microsome | associated with membranes, structural integrity of the microsomal membranes is essential for activity | Rattus norvegicus | - |
- |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
additional information | - |
C- and N-terminal amino acid sequences | Homo sapiens |
additional information | - |
C- and N-terminal amino acid sequences | Rattus norvegicus |
additional information | - |
enzyme forms dimeric or oligomeric complexes with another protein in the Golgi membrane vivo, topology | Homo sapiens |
additional information | - |
enzyme forms dimeric or oligomeric complexes with another protein in the Golgi membrane vivo, topology | Rattus norvegicus |
38000 | - |
x * 38000, SDS-PAGE | Homo sapiens |
38000 | - |
x * 38000, native and recombinant enzyme, SDS-PAGE | Rattus norvegicus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
UDP-glucose + N-acylsphingosine | Homo sapiens | - |
UDP + D-glucosyl-N-acylsphingosine | - |
? | |
UDP-glucose + N-acylsphingosine | Rattus norvegicus | initial key enzyme in glycosphingolipid biosynthesis | UDP + D-glucosyl-N-acylsphingosine | i.e. glucosylceramide | ? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Rattus norvegicus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
partial from Golgi membranes | Rattus norvegicus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
brain | - |
Homo sapiens | - |
brain | - |
Rattus norvegicus | - |
keratinocyte | - |
Homo sapiens | - |
liver | - |
Rattus norvegicus | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
UDP-glucose + N-acylsphingosine | - |
Homo sapiens | UDP + D-glucosyl-N-acylsphingosine | - |
? | |
UDP-glucose + N-acylsphingosine | i.e. ceramide | Homo sapiens | UDP + D-glucosyl-N-acylsphingosine | i.e. glucosylceramide | ? | |
UDP-glucose + N-acylsphingosine | i.e. ceramide | Rattus norvegicus | UDP + D-glucosyl-N-acylsphingosine | i.e. glucosylceramide | ? | |
UDP-glucose + N-acylsphingosine | initial key enzyme in glycosphingolipid biosynthesis | Rattus norvegicus | UDP + D-glucosyl-N-acylsphingosine | i.e. glucosylceramide | ? | |
UDP-glucose + N-[7-(4-nitrobenzo-2-oxa-1,3-diazole)]-6-aminocaproyl-D-erythro-sphingosine | synthetic fluorescent ceramide substrate analogue for fluorescence enzyme assay | Rattus norvegicus | UDP + D-glucosyl-N-[7-(4-nitrobenzo-2-oxa-1,3-diazole)]-6-aminocaproyl-D-erythro-sphingosine | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 38000, SDS-PAGE | Homo sapiens |
? | x * 38000, native and recombinant enzyme, SDS-PAGE | Rattus norvegicus |
Synonyms | Comment | Organism |
---|---|---|
GCS | - |
Homo sapiens |
GCS | - |
Rattus norvegicus |