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Literature summary for 2.4.1.80 extracted from
- Glucosylceramide synthase and glycosphingolipid synthesis (1998), Trends Cell Biol., 8, 198-202.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| dioleoyl phosphatidylcholine | or other exogenous phospholipid, required for activity | Rattus norvegicus |  |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
- |
Mus musculus |
| cloning and transient expression in GM-95 cell line, mutant cell line containing no enzyme activity, derived from melanoma mutant cell line B16, DNA sequence determination and analysis | Caenorhabditis elegans |
| cloning and transient expression in GM-95 cell line, mutant cell line containing no enzyme activity, derived from melanoma mutant cell line B16, DNA sequence determination and analysis | Homo sapiens |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| D-threo-1-phenyl-2-(decanoylamino)-3-morpholino-1-propanol-HCl | - |
Homo sapiens | |
| D-threo-1-phenyl-2-(decanoylamino)-3-morpholino-1-propanol-HCl | i.e. PDMP | Mus musculus | |
| D-threo-1-phenyl-2-(decanoylamino)-3-morpholino-1-propanol-HCl | i.e. PDMP | Rattus norvegicus | |
| N-butyldeoxynojirimycin | - |
Homo sapiens | |
| N-butyldeoxynojirimycin | - |
Mus musculus | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| Golgi membrane | orientation to the cytosolic side | Rattus norvegicus | 139 | - |
| microsome | - |
Mus musculus | - |
- |
| microsome | - |
Rattus norvegicus | - |
- |
| additional information | type III membrane protein | Rattus norvegicus | - |
- |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| additional information | - |
amino acid sequence alignment | Mus musculus |
| additional information | - |
amino acid sequence alignment | Rattus norvegicus |
| additional information | - |
amino acid sequence alignment | Caenorhabditis elegans |
| additional information | - |
amino acid sequence alignment | Homo sapiens |
| additional information | - |
type III membrane protein structure with N-terminal signal-anchor sequence and a long cytoplasmic tail | Rattus norvegicus |
| additional information | - |
type III membrane protein structure with N-terminal signal-anchor sequence and a long cytoplasmic tail | Homo sapiens |
| 45000 | - |
x * 45000, SDS-PAGE | Rattus norvegicus |
| 66000 | - |
sedimentation in a glycerol gradient | Rattus norvegicus |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| UDP-glucose + N-acylsphingosine | Mus musculus | ceramide plays an important role in signal transduction, regulation, and cell homeostasis, thus its glycosylation may play a role in regulation of the cellular level of the bioactive lipid | UDP + D-glucosyl-N-acylsphingosine | i.e. glucosylceramide | ? | |
| UDP-glucose + N-acylsphingosine | Rattus norvegicus | ceramide plays an important role in signal transduction, regulation, and cell homeostasis, thus its glycosylation may play a role in regulation of the cellular level of the bioactive lipid | UDP + D-glucosyl-N-acylsphingosine | i.e. glucosylceramide | ? | |
| UDP-glucose + N-acylsphingosine | Caenorhabditis elegans | ceramide plays an important role in signal transduction, regulation, and cell homeostasis, thus its glycosylation may play a role in regulation of the cellular level of the bioactive lipid | UDP + D-glucosyl-N-acylsphingosine | i.e. glucosylceramide | ? | |
| UDP-glucose + N-acylsphingosine | Homo sapiens | ceramide plays an important role in signal transduction, regulation, and cell homeostasis, thus its glycosylation may play a role in regulation of the cellular level of the bioactive lipid | UDP + D-glucosyl-N-acylsphingosine | i.e. glucosylceramide | ? | |
| UDP-glucose + N-acylsphingosine | Mus musculus | initial key enzyme in glycosphingolipid biosynthesis | UDP + D-glucosyl-N-acylsphingosine | i.e. glucosylceramide | ? | |
| UDP-glucose + N-acylsphingosine | Rattus norvegicus | initial key enzyme in glycosphingolipid biosynthesis | UDP + D-glucosyl-N-acylsphingosine | i.e. glucosylceramide | ? | |
| UDP-glucose + N-acylsphingosine | Caenorhabditis elegans | initial key enzyme in glycosphingolipid biosynthesis | UDP + D-glucosyl-N-acylsphingosine | i.e. glucosylceramide | ? | |
| UDP-glucose + N-acylsphingosine | Homo sapiens | initial key enzyme in glycosphingolipid biosynthesis | UDP + D-glucosyl-N-acylsphingosine | i.e. glucosylceramide | ? | |
| UDP-glucose + N-acylsphingosine | Mus musculus | enzyme activity is regulated developmentally | UDP + D-glucosyl-N-acylsphingosine | i.e. glucosylceramide | ? | |
| UDP-glucose + N-acylsphingosine | Rattus norvegicus | enzyme activity is regulated developmentally | UDP + D-glucosyl-N-acylsphingosine | i.e. glucosylceramide | ? | |
| UDP-glucose + N-acylsphingosine | Caenorhabditis elegans | enzyme activity is regulated developmentally | UDP + D-glucosyl-N-acylsphingosine | i.e. glucosylceramide | ? | |
| UDP-glucose + N-acylsphingosine | Homo sapiens | enzyme activity is regulated developmentally | UDP + D-glucosyl-N-acylsphingosine | i.e. glucosylceramide | ? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Caenorhabditis elegans | - |
several isoforms | - |
| Homo sapiens | Q16739 | - |
- |
| Mus musculus | - |
- |
- |
| Rattus norvegicus | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Rattus norvegicus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| liver | - |
Mus musculus | - |
| liver | - |
Rattus norvegicus | - |
| liver | - |
Homo sapiens | - |
| additional information | ubiquitous distribution | Rattus norvegicus | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| UDP-glucose + N-acylsphingosine | i.e. ceramide | Mus musculus | UDP + D-glucosyl-N-acylsphingosine | i.e. glucosylceramide | ? | |
| UDP-glucose + N-acylsphingosine | i.e. ceramide | Rattus norvegicus | UDP + D-glucosyl-N-acylsphingosine | i.e. glucosylceramide | ? | |
| UDP-glucose + N-acylsphingosine | i.e. ceramide | Caenorhabditis elegans | UDP + D-glucosyl-N-acylsphingosine | i.e. glucosylceramide | ? | |
| UDP-glucose + N-acylsphingosine | i.e. ceramide | Homo sapiens | UDP + D-glucosyl-N-acylsphingosine | i.e. glucosylceramide | ? | |
| UDP-glucose + N-acylsphingosine | ceramide plays an important role in signal transduction, regulation, and cell homeostasis, thus its glycosylation may play a role in regulation of the cellular level of the bioactive lipid | Mus musculus | UDP + D-glucosyl-N-acylsphingosine | i.e. glucosylceramide | ? | |
| UDP-glucose + N-acylsphingosine | ceramide plays an important role in signal transduction, regulation, and cell homeostasis, thus its glycosylation may play a role in regulation of the cellular level of the bioactive lipid | Rattus norvegicus | UDP + D-glucosyl-N-acylsphingosine | i.e. glucosylceramide | ? | |
| UDP-glucose + N-acylsphingosine | ceramide plays an important role in signal transduction, regulation, and cell homeostasis, thus its glycosylation may play a role in regulation of the cellular level of the bioactive lipid | Caenorhabditis elegans | UDP + D-glucosyl-N-acylsphingosine | i.e. glucosylceramide | ? | |
| UDP-glucose + N-acylsphingosine | ceramide plays an important role in signal transduction, regulation, and cell homeostasis, thus its glycosylation may play a role in regulation of the cellular level of the bioactive lipid | Homo sapiens | UDP + D-glucosyl-N-acylsphingosine | i.e. glucosylceramide | ? | |
| UDP-glucose + N-acylsphingosine | initial key enzyme in glycosphingolipid biosynthesis | Mus musculus | UDP + D-glucosyl-N-acylsphingosine | i.e. glucosylceramide | ? | |
| UDP-glucose + N-acylsphingosine | initial key enzyme in glycosphingolipid biosynthesis | Rattus norvegicus | UDP + D-glucosyl-N-acylsphingosine | i.e. glucosylceramide | ? | |
| UDP-glucose + N-acylsphingosine | initial key enzyme in glycosphingolipid biosynthesis | Caenorhabditis elegans | UDP + D-glucosyl-N-acylsphingosine | i.e. glucosylceramide | ? | |
| UDP-glucose + N-acylsphingosine | initial key enzyme in glycosphingolipid biosynthesis | Homo sapiens | UDP + D-glucosyl-N-acylsphingosine | i.e. glucosylceramide | ? | |
| UDP-glucose + N-acylsphingosine | enzyme activity is regulated developmentally | Mus musculus | UDP + D-glucosyl-N-acylsphingosine | i.e. glucosylceramide | ? | |
| UDP-glucose + N-acylsphingosine | enzyme activity is regulated developmentally | Rattus norvegicus | UDP + D-glucosyl-N-acylsphingosine | i.e. glucosylceramide | ? | |
| UDP-glucose + N-acylsphingosine | enzyme activity is regulated developmentally | Caenorhabditis elegans | UDP + D-glucosyl-N-acylsphingosine | i.e. glucosylceramide | ? | |
| UDP-glucose + N-acylsphingosine | enzyme activity is regulated developmentally | Homo sapiens | UDP + D-glucosyl-N-acylsphingosine | i.e. glucosylceramide | ? | |
| UDP-glucose + N-[7-(4-nitrobenzo-2-oxa-1,3-diazole)]-6-aminocaproyl-D-erythro-sphingosine | synthetic fluorescent ceramide substrate analogue for fluorescence enzyme assay | Rattus norvegicus | UDP + D-glucosyl-N-[7-(4-nitrobenzo-2-oxa-1,3-diazole)]-6-aminocaproyl-D-erythro-sphingosine | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | - |
Homo sapiens |
| ? | x * 45000, SDS-PAGE | Rattus norvegicus |
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