Cloned (Comment) | Organism |
---|---|
cloning and transient expression in GM-95 cell line, mutant cell line containing no enzyme activity, derived from melanoma mutant cell line B16, DNA sequence determination and analysis | Homo sapiens |
functional expression in Escherichia coli BL21(DE3) | Homo sapiens |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
additional information | - |
amino acid sequence | Homo sapiens |
448530 | - |
x * 448530, amino acid sequence determination | Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
UDP-glucose + N-acylsphingosine | Homo sapiens | first step in synthesis of gangliosides | UDP + D-glucosyl-N-acylsphingosine | i.e. glucosylceramide | ? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | Q16739 | - |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
brain | mRNA | Homo sapiens | - |
heart | mRNA | Homo sapiens | - |
kidney | mRNA | Homo sapiens | - |
liver | mRNA | Homo sapiens | - |
lung | mRNA | Homo sapiens | - |
pancreas | mRNA | Homo sapiens | - |
placenta | mRNA | Homo sapiens | - |
skeletal muscle | mRNA | Homo sapiens | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
UDP-glucose + N-acylsphingosine | i.e. ceramide | Homo sapiens | UDP + D-glucosyl-N-acylsphingosine | i.e. glucosylceramide | ? | |
UDP-glucose + N-acylsphingosine | first step in synthesis of gangliosides | Homo sapiens | UDP + D-glucosyl-N-acylsphingosine | i.e. glucosylceramide | ? |
Subunits | Comment | Organism |
---|---|---|
? | x * 448530, amino acid sequence determination | Homo sapiens |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Homo sapiens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
assay at | Homo sapiens |