Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 2.4.1.8 extracted from

  • Hidaka, Y.; Hatada, Y.; Akita, M.; Yoshida, M.; Nakamura, N.; Takada, M.; Nakakuki, T.; Ito, S.; Horikoshi, K.
    Maltose phosphorylase from a deep-sea Paenibacillus sp.: Enzymatic properties and nucleotide and amino-acid sequences (2005), Enzyme Microb. Technol., 37, 185-194.
No PubMed abstract available
Search for more literature for 2.4.1.8

Cloned(Commentary)

Cloned (Comment) Organism
overexpressed in Escherichia coli Paenibacillus sp.

Protein Variants

Protein Variants Comment Organism
E487A mutant loses both the phosphorolytic and the synthetic activity Paenibacillus sp.
E487Q mutant loses the phosphorolytic activity, but retains the synthetic activity (13% of the activity of wild-type MapA) Paenibacillus sp.
S634V mutant loses both the phosphorolytic and the synthetic activity Paenibacillus sp.
S635V mutant loses both the phosphorolytic and the synthetic activity Paenibacillus sp.
Y351F mutant loses both the phosphorolytic and the synthetic activity Paenibacillus sp.

Inhibitors

Inhibitors Comment Organism Structure
4-chloromercuribenzoate
-
 Paenibacillus sp.
Cu2+ 1 mM, 50°C, 30 min, abolishes the phosphorolytic activity almost completely Paenibacillus sp.
diethyldicarbonate
-
 Paenibacillus sp.
Hg2+ 1 mM, 50°C, 30 min, abolishes the phosphorolytic activity almost completely Paenibacillus sp.
additional information iodoacetoamide, EDTA, and phenylmethanesulfonyl fluoride have no inhibitory effect Paenibacillus sp.
N-bromosuccinimide 0.1 mM, 47% inhibition Paenibacillus sp.
N-ethylmaleimide
-
 Paenibacillus sp.

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0036
-
 beta-D-glucose 1-phosphate 50°C, pH 7.0, mutant enzyme E487Q Paenibacillus sp.
0.004
-
 maltose 50°C, pH 7.0, wild-type enzyme Paenibacillus sp.
0.0043
-
 D-glucose 50°C, pH 7.0, mutant enzyme E487Q Paenibacillus sp.
0.0067
-
 beta-D-glucose 1-phosphate 50°C, pH 7.0, wild-type enzyme Paenibacillus sp.
0.0071
-
 phosphate 50°C, pH 7.0, wild-type enzyme Paenibacillus sp.
0.0099
-
 D-glucose 50°C, pH 7.0, wild-type enzyme Paenibacillus sp.

Metals/Ions

Metals/Ions Comment Organism Structure
Fe3+ 1 mM, 50°C, 30 min, enhances the activity moderately Paenibacillus sp.
Mn2+ 1 mM, 50°C, 30 min, enhances the activity moderately Paenibacillus sp.
Pb2+ 1 mM, 50°C, 30 min, enhances the activity moderately Paenibacillus sp.
Sn2+ 1 mM, 50°C, 30 min, enhances the activity moderately Paenibacillus sp.

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
87748
-
 2 * 87748, calculated from sequence Paenibacillus sp.
90000
-
 2 * 90000, SDS-PAGE Paenibacillus sp.
180000
-
 gel filtration Paenibacillus sp.

Organism

Organism UniProt Comment Textmining
Paenibacillus sp. Q50LH0
-
-
Paenibacillus sp. SH-55 Q50LH0
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Paenibacillus sp.

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
50
-
-
 Paenibacillus sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
maltose + phosphate
-
 Paenibacillus sp. D-glucose + beta-D-glucose 1-phosphate
-
 r
maltose + phosphate
-
 Paenibacillus sp. SH-55 D-glucose + beta-D-glucose 1-phosphate
-
 r
additional information no activity with: isomaltose, trehalose, neotrehalose, sucrose, lactose, and cellobiose Paenibacillus sp. ?
-
 ?
additional information no activity with: isomaltose, trehalose, neotrehalose, sucrose, lactose, and cellobiose Paenibacillus sp. SH-55 ?
-
 ?

Subunits

Subunits Comment Organism
dimer 2 * 90000, SDS-PAGE Paenibacillus sp.
dimer 2 * 87748, calculated from sequence Paenibacillus sp.

Synonyms

Synonyms Comment Organism
mpA
-
 Paenibacillus sp.

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
40
-
 glycosynthesis Paenibacillus sp.
50
-
 phosphorolysis Paenibacillus sp.

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
50
-
 15 min, pH 7.0, stable up to Paenibacillus sp.
55
-
 15 min, pH 7.0, 70% of the original activity is retained Paenibacillus sp.

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.5
-
 beta-D-glucose 1-phosphate 50°C, pH 7.0, mutant enzyme E487Q Paenibacillus sp.
3.8
-
 D-glucose 50°C, pH 7.0, mutant enzyme E487Q Paenibacillus sp.
12.9
-
 phosphate 50°C, pH 7.0, wild-type enzyme Paenibacillus sp.
17.3
-
 maltose 50°C, pH 7.0, wild-type enzyme Paenibacillus sp.
43.5
-
 beta-D-glucose 1-phosphate 50°C, pH 7.0, wild-type enzyme Paenibacillus sp.
49.4
-
 D-glucose 50°C, pH 7.0, wild-type enzyme Paenibacillus sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6
-
 glycosynthesis Paenibacillus sp.
7 7.5 phosphorolysis Paenibacillus sp.

pH Range

pH Minimum pH Maximum Comment Organism
5 8 more than 50% of maximal activity, phosphorolysis Paenibacillus sp.
6 7 more than 50% of maximal activity, glycosynthesis Paenibacillus sp.

pH Stability

pH Stability pH Stability Maximum Comment Organism
5.5 6 15 min, 50°C, most stable in the pH-range 5.5-6.0 Paenibacillus sp.
5.5 7 15 min, 50°C, retains 50% of the original activity in the pH-range 5.5-7.0 Paenibacillus sp.