General Stability | Organism |
---|---|
ammonium sulfate at high concentrations inactivates | Glycine max |
freeze-thawing, stable to | Glycine max |
stable to centrifugation at 150000 rpm for 90 min | Glycine max |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
catechol | weak | Glycine max | |
CuSO4 | strong | Glycine max | |
GSSG | oxidized glutathione, weak | Glycine max | |
HgCl2 | strong | Glycine max | |
iodoacetamide | weak | Glycine max | |
additional information | no inhibition by EDTA and iodoacetate, 1 mM each | Glycine max | |
NEM | - |
Glycine max | |
o-Iodosobenzoate | - |
Glycine max | |
p-benzoquinone | strong | Glycine max | |
PCMB | strong | Glycine max | |
Sodium arsenate | 1 mM, weak | Glycine max | |
Tris buffer | - |
Glycine max | |
UDP | linear competitive inhibitor to UDPglucose | Glycine max |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.563 | - |
3,4-dichloroaniline | - |
Glycine max | |
1.88 | - |
UDPglucose | - |
Glycine max |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytosol | - |
Glycine max | 5829 | - |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
UDP-glucose + an arylamine | Glycine max | involved in N-glucosylarylamines biosynthesis, may be participate in arylamine herbicide metabolism | UDP + an N-D-glucosylarylamine | - |
? |
Organic Solvent | Comment | Organism |
---|---|---|
Acetone | inactivates | Glycine max |
Ethanol | inactivates | Glycine max |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Glycine max | - |
Merril, var. Hawkeye | - |
Purification (Comment) | Organism |
---|---|
partial | Glycine max |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
cotyledon | - |
Glycine max | - |
hypocotyl | - |
Glycine max | - |
leaf | - |
Glycine max | - |
root | - |
Glycine max | - |
seed | - |
Glycine max | - |
seedling | etiolated | Glycine max | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
activity in different tissues | Glycine max |
0.000912 | - |
- |
Glycine max |
Storage Stability | Organism |
---|---|
-15°C, lyophilized, several months | Glycine max |
4°C, t1/2: 48 h | Glycine max |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
TDP-glucose + an arylamine | - |
Glycine max | TDP + an N-D-glucosylarylamine | - |
? | |
UDP-glucose + 2,3-dichloroaniline | - |
Glycine max | UDP + N-(2,3-dichlorophenyl)-glucosylamine | - |
? | |
UDP-glucose + 2,4-dibromoaniline | - |
Glycine max | UDP + N-(2,4-dibromophenyl)-glucosylamine | - |
? | |
UDP-glucose + 2,4-dichloroaniline | - |
Glycine max | UDP + N-(2,4-dichlorophenyl)-glucosylamine | - |
? | |
UDP-glucose + 2,5-dibromoaniline | - |
Glycine max | UDP + N-(2,5-dibromophenyl)-glucosylamine | - |
? | |
UDP-glucose + 2,5-dichloroaniline | - |
Glycine max | UDP + N-(2,5-dichlorophenyl)-glucosylamine | - |
? | |
UDP-glucose + 3,4-dichloroaniline | best glucosyl acceptor, no glucosyl donors are GDP-glucose, CDP-glucose, ADP-glucose, UDP-mannose | Glycine max | UDP + N-(3,4-dichlorophenyl)-glucosylamine | - |
? | |
UDP-glucose + 3,5-dichloroaniline | - |
Glycine max | UDP + N-(3,5-dichlorophenyl)-glucosylamine | - |
? | |
UDP-glucose + 3-amino-2,5-dichlorobenzoic acid | - |
Glycine max | UDP + 3-glucosylamino-2,5-dichlorobenzoic acid | - |
? | |
UDP-glucose + 3-aminobenzoic acid | - |
Glycine max | UDP + 3-glucosylaminobenzoic acid | - |
? | |
UDP-glucose + 3-chloroaniline | 31% efficiency compared to 3,4-dichloroaniline | Glycine max | UDP + N-(3-chlorophenyl)-glucosylamine | - |
? | |
UDP-glucose + 3-nitroaniline | - |
Glycine max | UDP + N-(3-nitrophenyl)-glucosylamine | - |
? | |
UDP-glucose + 4-bromoaniline | 28% efficiency compared to 3,4-dichloroaniline | Glycine max | UDP + N-(4-bromophenyl)-glucosylamine | - |
? | |
UDP-glucose + 4-chloroaniline | 71% efficiency compared to 3,4-dichloroaniline | Glycine max | UDP + N-(4-chlorophenyl)-glucosylamine | - |
? | |
UDP-glucose + 5-amino-2,3-dichlorobenzoic acid | - |
Glycine max | UDP + 5-glucosylamino-2,3-dichlorobenzoic acid | - |
? | |
UDP-glucose + an arylamine | involved in N-glucosylarylamines biosynthesis, may be participate in arylamine herbicide metabolism | Glycine max | UDP + an N-D-glucosylarylamine | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
assay at | Glycine max |
28 | - |
assay at | Glycine max |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
80% loss of activity within 10 min | Glycine max |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.4 | 7.8 | - |
Glycine max |
7.5 | - |
with 3,4-dichloroaniline as acceptor | Glycine max |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.484 | - |
UDP | - |
Glycine max |