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Literature summary for 2.4.1.7 extracted from
- Dissecting differential binding of fructose and phosphate as leaving group/nucleophile of glucosyl transfer catalyzed by sucrose phosphorylase (2007), FEBS Lett., 581, 3814-3818.
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| D338N | site-directed mutagenesis of a fructose-binding residue, the mutant shows 7000fold reduced activity compared to the wild-type enzyme due to disruption of steps where fructose departs or attacks | Leuconostoc mesenteroides |
| R137A | site-directed mutagenesis of a phosphate-binding residue, the mutant shows 60fold reduced activity compared to the wild-type enzyme due to disruption of steps where fructose departs or attacks | Leuconostoc mesenteroides |
| Y340A | site-directed mutagenesis of a phosphate-binding residue, the mutant shows 2500fold reduced activity compared to the wild-type enzyme due to disruption of steps where fructose departs or attacks | Leuconostoc mesenteroides |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | kinetics of recombinant wild-type and mutant enzymes, overview | Leuconostoc mesenteroides |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| sucrose + phosphate | Leuconostoc mesenteroides | - |
D-fructose + alpha-D-glucose 1-phosphate | - |
r |  |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Leuconostoc mesenteroides | - |
- |
- |
Reaction
| Reaction | Comment | Organism | Reaction ID |
|---|---|---|---|
| sucrose + phosphate = D-fructose + alpha-D-glucose 1-phosphate | reaction mechanism | Leuconostoc mesenteroides |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
- |
Leuconostoc mesenteroides |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| alpha-D-glucose 1-phosphate + arsenate | - |
Leuconostoc mesenteroides | ? | - |
? | |
| sucrose + phosphate | - |
Leuconostoc mesenteroides | D-fructose + alpha-D-glucose 1-phosphate | - |
r | |
| sucrose + phosphate | catalytic mechanisms of wild-type and mutant enzymes, overview | Leuconostoc mesenteroides | D-fructose + alpha-D-glucose 1-phosphate | - |
r | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| SPase | - |
Leuconostoc mesenteroides |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
assay at | Leuconostoc mesenteroides |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 72 | - |
D-fructose | pH 7.0, 30°C, synthesis, wild-type enzyme | Leuconostoc mesenteroides | |
| 91 | - |
alpha-D-glucose 1-phosphate | pH 7.0, 30°C, synthesis, wild-type enzyme | Leuconostoc mesenteroides | |
| 170 | - |
arsenate | pH 7.0, 30°C, arsenolysis, wild-type enzyme | Leuconostoc mesenteroides | |
| 170 | - |
sucrose | pH 7.0, 30°C, phosphorolysis, wild-type enzyme | Leuconostoc mesenteroides | |
| 200 | - |
phosphate | pH 7.0, 30°C, phosphorolysis, wild-type enzyme | Leuconostoc mesenteroides | |
| 260 | - |
alpha-D-glucose 1-phosphate | pH 7.0, 30°C, arsenolysis, wild-type enzyme | Leuconostoc mesenteroides | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | - |
assay at | Leuconostoc mesenteroides |
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Release 2023.1 (January 2023)
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