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Literature summary for 2.4.1.69 extracted from

  • Sarnesto, A.; Koehlin, T.; Hindsgaul, O.; Thurin, J.; Blaszczyk-Thurin, M.
    Purification of the secretor-type beta-galactoside alpha 1->2-fucosyltransferase from human serum (1992), J. Biol. Chem., 267, 2737-2744.
    View publication on PubMed
Search for more literature for 2.4.1.69

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.027
-
 GDP-L-fucose H-gene encoded enzyme Homo sapiens
0.108
-
 GDP-L-fucose Se-encoded enzyme Homo sapiens
1.4
-
 lacto-N-biose I Se-gene encoded enzyme Homo sapiens
1.4
-
 Galbeta(1-3)GalNAc Se-gene encoded enzyme Homo sapiens
1.4
-
 lacto-N-tetraose Se-gene encoded enzyme Homo sapiens
1.9
-
 N-acetyl-beta-lactosamine H-gene encoded enzyme Homo sapiens
2.1
-
 lactose H-gene encoded enzyme Homo sapiens
2.2
-
 lacto-N-tetraose H-gene encoded enzyme Homo sapiens
2.3
-
 Galbeta(1-3)GalNAc Se-gene encoded enzyme Homo sapiens
2.9
-
 beta-D-galactose H-gene encoded enzyme Homo sapiens
2.9
-
 Galbeta(1-3)GalNAc H-gene encoded enzyme Homo sapiens
3
-
 phenyl-beta-D-galactoside H-gene encoded enzyme Homo sapiens
3.2
-
 beta-D-galactose-beta-O-2-(2-carbomethoxyethylthio)ethyl H-gene encoded enzyme Homo sapiens
3.4
-
 lacto-N-neotetraose H-gene encoded enzyme Homo sapiens
3.5
-
 lacto-N-biose I H-gene encoded enzyme Homo sapiens
4.2
-
 Galbeta1-6GalNAc H-gene encoded enzyme Homo sapiens
5.5
-
 lactose Se-gene encoded enzyme Homo sapiens
5.5
-
 lacto-N-neotetraose Se-gene encoded enzyme Homo sapiens
5.7
-
 N-acetyl-beta-lactosamine Se-gene encoded enzyme Homo sapiens
8.5
-
 beta-D-galactose-beta-O-2-(2-carbomethoxyethylthio)ethyl Se-gene encoded enzyme Homo sapiens
11.4
-
 phenyl-beta-D-galactoside Se-gene encoded enzyme Homo sapiens
24
-
 beta-D-galactose Se-gene encoded enzyme Homo sapiens

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+
-
 Homo sapiens

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
50000
-
 x * 50000, enzyme from serum, SDS-PAGE under reducing conditions Homo sapiens
150000
-
 gel filtration Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Purification (Commentary)

Purification (Comment) Organism
enzyme from serum Homo sapiens

Source Tissue

Source Tissue Comment Organism Textmining
blood serum
-
 Homo sapiens
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
188
-
-
 Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
GDP-beta-L-fucose + beta-D-galactose
-
 Homo sapiens ?
-
 ?
GDP-L-fucose + beta-D-galactose-beta-O-2-(2-carbomethoxyethylthio)ethyl
-
 Homo sapiens ?
-
 ?
GDP-L-fucose + beta-D-galactosyl-R
-
 Homo sapiens GDP + alpha-L-fucosyl-1,2-beta-D-galactosyl-R
-
 ?
GDP-L-fucose + D-galactose
-
 Homo sapiens GDP + alpha-L-fucosyl-1,2-D-galactose
-
 ?
GDP-L-fucose + Galbeta(1-3)GalNAc
-
 Homo sapiens GDP + L-Fucalpha(1-2)Galbeta(1-3)GalNAc
-
 ?
GDP-L-fucose + Galbeta(1-6)GalNAc
-
 Homo sapiens GDP + L-Fucalpha(1-2)Galbeta(1-6)GalNAc
-
 ?
GDP-L-fucose + lacto-N-biose 1
-
 Homo sapiens GDP + L-Fucalpha(1-2)Galbeta(1-3)GlcNAc
-
 ?
GDP-L-fucose + lacto-N-neotetraose
-
 Homo sapiens GDP + lacto-N-fucopentaose IV
-
 ?
GDP-L-fucose + lacto-N-tetraose
-
 Homo sapiens GDP + lacto-N-fucopentaose I
-
 ?
GDP-L-fucose + lactose
-
 Homo sapiens GDP + fucosyllactose
-
 ?
GDP-L-fucose + N-acetyllactosamine
-
 Homo sapiens GDP + alpha-L-fucosyl-1,2-N-acetyllactosamine
-
 ?
GDP-L-fucose + phenyl-beta-D-galactoside
-
 Homo sapiens GDP + phenyl 2-O-(alpha-L-fucopyranosyl)-beta-D-galactopyranoside
-
 ?
additional information secretor-type alpha1-2 fucosyltransferase shows significant lower affinity than the H-gene encoded enzyme for phenyl-beta-D-galactopyranoside and GDP-L-fucose as well as for type 1 oligosaccharide acceptors, Galbeta1-4GlcNAc. Type 1 acceptors, Galbeta1-3GlcNAc, and type 3 acceptors, Galbeta1-3GalNAc, are preferentially used by the secretor-type enzyme as compared with the H enzyme Homo sapiens ?
-
 ?

Subunits

Subunits Comment Organism
? x * 50000, enzyme from serum, SDS-PAGE under reducing conditions Homo sapiens