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Literature summary for 2.4.1.69 extracted from

  • Sarnesto, A.; Koehlin, T.; Thurin, J.; Blaszczyk-Thurin, M.
    Purification of Hgene-encoded beta-galactoside alpha 1->2 fucosyltransferase from human serum (1990), J. Biol. Chem., 265, 15067-15075.
    View publication on PubMed
Search for more literature for 2.4.1.69

Inhibitors

Inhibitors Comment Organism Structure
2-mercaptoethanol 1 mM: 50% loss of activity, 10 mM: 90% inhibition Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.6
-
 Galbeta(1-3)GlcNAcbeta(1-3)Galbeta(1-4)Glc-beta-O-PA8 H-gene encoded enzyme Homo sapiens
2.5
-
 Galbeta(1-4)GlcNAc-beta-O-2-(2-carbomethoxyethylthio)ethyl H-endoced and Se-encoded enzyme Homo sapiens
2.7
-
 phenyl-beta-O-galactoside H-gene encoded enzyme Homo sapiens
2.9
-
 Galbeta(1-4)Glc-beta-O-2-(2-carbomethoxyethylthio)ethyl H-encoded enzyme Homo sapiens
3.1
-
 Galbeta(1-4)Glc-beta-O-2-(2-carbomethoxyethylthio)ethyl Se-encoded enzyme Homo sapiens
8.4
-
 phenyl-beta-O-galactoside Se-gene encoded enzyme Homo sapiens

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
50000
-
 x * 50000, enzyme from serum, SDS-PAGE under reducing conditions Homo sapiens
148000
-
 gel filtration Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein
-
 Homo sapiens

Purification (Commentary)

Purification (Comment) Organism
enzyme from serum Homo sapiens

Source Tissue

Source Tissue Comment Organism Textmining
blood serum from AB and mixed secretor phenotype individuals Homo sapiens
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
 Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
GDP-beta-L-fucose + Galbeta(1-3)GlcNAcbeta(1-3)Galbeta(1-4)Glc-beta-O-PA8
-
 Homo sapiens ?
-
 ?
GDP-L-fucose + beta-D-galactosyl-R
-
 Homo sapiens GDP + alpha-L-fucosyl-1,2-beta-D-galactosyl-R
-
 ?

Subunits

Subunits Comment Organism
? x * 50000, enzyme from serum, SDS-PAGE under reducing conditions Homo sapiens