Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Cu2+ | - |
Vigna angularis | |
D-ononitol | competitive inhibition when the enzyme is incubated with both raffinose and D-ononitol simultaneously | Vigna angularis | |
Fe2+ | - |
Vigna angularis | |
Mn2+ | - |
Vigna angularis | |
raffinose | competitive inhibition when the enzyme is incubated with both raffinose and D-ononitol simultaneously | Vigna angularis | |
Zn2+ | - |
Vigna angularis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.8 | - |
1-alpha-D-galactosyl-myo-inositol | with raffinose | Vigna angularis | |
2.4 | - |
raffinose | with 1-alpha-D-galactosyl-myo-inositol at 1.1 mM | Vigna angularis | |
5.5 | - |
myo-inositol | with 10 mM 1-alpha-D-galactosyl-myo-inositol in the exchange reaction | Vigna angularis | |
13.2 | - |
raffinose | with galactosylononitol at nonsaturating level | Vigna angularis | |
31.3 | - |
alpha-D-galactosyl-D-ononitol | - |
Vigna angularis | |
38.6 | - |
raffinose | with 1-alpha-D-galactosyl-myo-inositol at nonsaturating level | Vigna angularis |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
dithiothreitol | activation | Vigna angularis | |
EDTA | slight stimulation | Vigna angularis | |
Mg2+ | slight stimulation | Vigna angularis |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
additional information | - |
enzyme forms a 283 kDa aggregate during gel filtration step | Vigna angularis |
90100 | - |
1 * 90100, SDS-PAGE | Vigna angularis |
110000 | - |
gel filtration | Vigna angularis |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
1-alpha-D-galactosyl-myo-inositol + D-ononitol | Vigna angularis | biosynthesis of galactosylononitol | myo-inositol + alpha-D-galactosyl-D-ononitol | - |
? | |
1-alpha-D-galactosyl-myo-inositol + raffinose | Vigna angularis | - |
myo-inositol + stachyose | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Vigna angularis | - |
adzuki bean | - |
Purification (Comment) | Organism |
---|---|
- |
Vigna angularis |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
seed | - |
Vigna angularis | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.67 | - |
purified enzyme | Vigna angularis |
1.3 | - |
purified enzyme, substrates: 1-alpha-D-galactosyl-myo-inositol + D-ononitol | Vigna angularis |
Storage Stability | Organism |
---|---|
-196°C, stored in liquid N2, stable for at least 4 months | Vigna angularis |
-20°C, loss of 85% activity within 4 months | Vigna angularis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
1-alpha-D-galactosyl-myo-inositol + D-ononitol | ononitol is 1-D-4-O-methyl-myo-inositol | Vigna angularis | myo-inositol + alpha-D-galactosyl-D-ononitol | - |
r | |
1-alpha-D-galactosyl-myo-inositol + D-ononitol | biosynthesis of galactosylononitol | Vigna angularis | myo-inositol + alpha-D-galactosyl-D-ononitol | - |
? | |
1-alpha-D-galactosyl-myo-inositol + myo-inositol | exchange reaction | Vigna angularis | myo-inositol + 1-alpha-D-galactosyl-myo-inositol | - |
r | |
1-alpha-D-galactosyl-myo-inositol + myo-inositol | several isomeric inositols and inositol O-methyl-ethers are substrates, some are inactive, overview | Vigna angularis | myo-inositol + 1-alpha-D-galactosyl-myo-inositol | - |
r | |
1-alpha-D-galactosyl-myo-inositol + myo-inositol | 2.25fold increased activity compared to raffinose | Vigna angularis | myo-inositol + 1-alpha-D-galactosyl-myo-inositol | - |
r | |
1-alpha-D-galactosyl-myo-inositol + raffinose | - |
Vigna angularis | myo-inositol + stachyose | - |
? | |
1-alpha-D-galactosyl-myo-inositol + raffinose | i.e. galactinol | Vigna angularis | myo-inositol + stachyose | - |
? | |
1-alpha-D-galactosyl-myo-inositol + sequoyitol | sequoyitol is 5-O-methyl-myo-inositol | Vigna angularis | myo-inositol + alpha-D-galactosyl-sequoyitol | - |
? | |
1-alpha-D-galactosyl-myo-inositol + sequoyitol | 1.3fold increased activity compared to raffinose | Vigna angularis | myo-inositol + alpha-D-galactosyl-sequoyitol | - |
? | |
alpha-D-galactosyl-D-ononitol + raffinose | - |
Vigna angularis | D-ononitol + stachyose | - |
? | |
additional information | substrate specificity, overview | Vigna angularis | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 90100, SDS-PAGE | Vigna angularis |
Synonyms | Comment | Organism |
---|---|---|
stachyose synthase | - |
Vigna angularis |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
35 | - |
- |
Vigna angularis |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
23 | 35 | half-maximal activity at 23°C | Vigna angularis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
additional information | - |
several active forms with pI values between 4.7 and 5.0 | Vigna angularis |
6.5 | 7 | - |
Vigna angularis |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
additional information | - |
unstable at low pH-values | Vigna angularis |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
17.9 | - |
raffinose | competitive against D-ononitol | Vigna angularis | |
18.7 | - |
D-ononitol | competitive against raffinose | Vigna angularis |