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Literature summary for 2.4.1.67 extracted from

  • Peterbauer, T.; Richter, A.
    Galactosylononitol and stachyose synthesis in seeds of adzuki bean. Purification and characterization of stachyose synthase (1998), Plant Physiol., 117, 165-172.
    View publication on PubMedView publication on EuropePMC

Inhibitors

Inhibitors Comment Organism Structure
Cu2+
-
Vigna angularis
D-ononitol competitive inhibition when the enzyme is incubated with both raffinose and D-ononitol simultaneously Vigna angularis
Fe2+
-
Vigna angularis
Mn2+
-
Vigna angularis
raffinose competitive inhibition when the enzyme is incubated with both raffinose and D-ononitol simultaneously Vigna angularis
Zn2+
-
Vigna angularis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.8
-
1-alpha-D-galactosyl-myo-inositol with raffinose Vigna angularis
2.4
-
raffinose with 1-alpha-D-galactosyl-myo-inositol at 1.1 mM Vigna angularis
5.5
-
myo-inositol with 10 mM 1-alpha-D-galactosyl-myo-inositol in the exchange reaction Vigna angularis
13.2
-
raffinose with galactosylononitol at nonsaturating level Vigna angularis
31.3
-
alpha-D-galactosyl-D-ononitol
-
Vigna angularis
38.6
-
raffinose with 1-alpha-D-galactosyl-myo-inositol at nonsaturating level Vigna angularis

Metals/Ions

Metals/Ions Comment Organism Structure
dithiothreitol activation Vigna angularis
EDTA slight stimulation Vigna angularis
Mg2+ slight stimulation Vigna angularis

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
additional information
-
enzyme forms a 283 kDa aggregate during gel filtration step Vigna angularis
90100
-
1 * 90100, SDS-PAGE Vigna angularis
110000
-
gel filtration Vigna angularis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1-alpha-D-galactosyl-myo-inositol + D-ononitol Vigna angularis biosynthesis of galactosylononitol myo-inositol + alpha-D-galactosyl-D-ononitol
-
?
1-alpha-D-galactosyl-myo-inositol + raffinose Vigna angularis
-
myo-inositol + stachyose
-
?

Organism

Organism UniProt Comment Textmining
Vigna angularis
-
adzuki bean
-

Purification (Commentary)

Purification (Comment) Organism
-
Vigna angularis

Source Tissue

Source Tissue Comment Organism Textmining
seed
-
Vigna angularis
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.67
-
purified enzyme Vigna angularis
1.3
-
purified enzyme, substrates: 1-alpha-D-galactosyl-myo-inositol + D-ononitol Vigna angularis

Storage Stability

Storage Stability Organism
-196°C, stored in liquid N2, stable for at least 4 months Vigna angularis
-20°C, loss of 85% activity within 4 months Vigna angularis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1-alpha-D-galactosyl-myo-inositol + D-ononitol ononitol is 1-D-4-O-methyl-myo-inositol Vigna angularis myo-inositol + alpha-D-galactosyl-D-ononitol
-
r
1-alpha-D-galactosyl-myo-inositol + D-ononitol biosynthesis of galactosylononitol Vigna angularis myo-inositol + alpha-D-galactosyl-D-ononitol
-
?
1-alpha-D-galactosyl-myo-inositol + myo-inositol exchange reaction Vigna angularis myo-inositol + 1-alpha-D-galactosyl-myo-inositol
-
r
1-alpha-D-galactosyl-myo-inositol + myo-inositol several isomeric inositols and inositol O-methyl-ethers are substrates, some are inactive, overview Vigna angularis myo-inositol + 1-alpha-D-galactosyl-myo-inositol
-
r
1-alpha-D-galactosyl-myo-inositol + myo-inositol 2.25fold increased activity compared to raffinose Vigna angularis myo-inositol + 1-alpha-D-galactosyl-myo-inositol
-
r
1-alpha-D-galactosyl-myo-inositol + raffinose
-
Vigna angularis myo-inositol + stachyose
-
?
1-alpha-D-galactosyl-myo-inositol + raffinose i.e. galactinol Vigna angularis myo-inositol + stachyose
-
?
1-alpha-D-galactosyl-myo-inositol + sequoyitol sequoyitol is 5-O-methyl-myo-inositol Vigna angularis myo-inositol + alpha-D-galactosyl-sequoyitol
-
?
1-alpha-D-galactosyl-myo-inositol + sequoyitol 1.3fold increased activity compared to raffinose Vigna angularis myo-inositol + alpha-D-galactosyl-sequoyitol
-
?
alpha-D-galactosyl-D-ononitol + raffinose
-
Vigna angularis D-ononitol + stachyose
-
?
additional information substrate specificity, overview Vigna angularis ?
-
?

Subunits

Subunits Comment Organism
monomer 1 * 90100, SDS-PAGE Vigna angularis

Synonyms

Synonyms Comment Organism
stachyose synthase
-
Vigna angularis

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
35
-
-
Vigna angularis

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
23 35 half-maximal activity at 23°C Vigna angularis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
additional information
-
several active forms with pI values between 4.7 and 5.0 Vigna angularis
6.5 7
-
Vigna angularis

pH Stability

pH Stability pH Stability Maximum Comment Organism
additional information
-
unstable at low pH-values Vigna angularis

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
17.9
-
raffinose competitive against D-ononitol Vigna angularis
18.7
-
D-ononitol competitive against raffinose Vigna angularis