Any feedback?
Literature summary for 2.4.1.65 extracted from
- Molecular cloning and functional expression of a novel Helicobacter pylori alpha-1,4 fucosyltransferase (2005), Glycobiology, 15, 1076-1083.
Application
| Application | Comment | Organism |
|---|---|---|
| synthesis | the enzyme possesses a broad tolerance toward nonnatural type I acceptor substrate analogs and therefore represents a valuable tool for the chemoenzymatic synthesis of Lewis A, sialyl Lewis A as well as mimetics thereof | Helicobacter pylori |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in Escherichia coli | Helicobacter pylori |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| NEM | 10 mM, 90% inhibition | Helicobacter pylori |  |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.00573 | - |
GDP-beta-L-fucose | 37°C, pH 6.5 | Helicobacter pylori | |
| 0.111 | - |
Siaalpha-2,3Galbeta-1,3GlcNAc-R | 37°C, pH 6.5 | Helicobacter pylori | |
| 0.114 | - |
Galbeta-1,3GlcNAc-R | 37°C, pH 6.5 | Helicobacter pylori | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 50502 | - |
x * 50502, SDS-PAGE | Helicobacter pylori |
| 64500 | - |
x * 64500, SDS-PAGE | Helicobacter pylori |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Helicobacter pylori | Q6ST35 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant | Helicobacter pylori |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -70°C, recombinant enzyme is stable for several months in cacodylate buffer containing | Helicobacter pylori |
| 10% glycerol. | Helicobacter pylori |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| GDP-beta-L-fucose + Gal-beta-(1->3)-GlcNAc-beta-O-(CH2)8COOCH3 | - |
Helicobacter pylori | GDP + Gal-beta-(1->3)-[Fuc-alpha-(1->4)]-GlcNAc-beta-O-(CH2)8COOCH3 | - |
? | |
| GDP-beta-L-fucose + Galbeta-1,3GlcNAc-R | - |
Helicobacter pylori | ? | - |
? | |
| GDP-beta-L-fucose + Galbeta1,3GlcNAc-O(CH2)8CO2CH3 | - |
Helicobacter pylori | ? | - |
? | |
| GDP-beta-L-fucose + NeuAcalpha1-3Galbeta1-3GlcNAc-beta-O-(CH2)8COOCH3 | - |
Helicobacter pylori | ? | - |
? | |
| GDP-beta-L-fucose + Siaalpha-2,3Galbeta-1,3GlcNAc-R | - |
Helicobacter pylori | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 64500, SDS-PAGE | Helicobacter pylori |
| ? | x * 50502, SDS-PAGE | Helicobacter pylori |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| alpha-1,4 fucosyltransferase | - |
Helicobacter pylori |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | 42 | - |
Helicobacter pylori |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 25 | 50 | 25°C: 70% of maximal activity, 50°C: about 60% of maximal activity | Helicobacter pylori |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 3.13 | - |
GDP-beta-L-fucose | 37°C, pH 6.5 | Helicobacter pylori | |
| 3.947 | - |
GDP-beta-L-fucose | 37°C, pH 6.5 | Helicobacter pylori | |
| 8.1 | - |
Galbeta-(1-3)-GlcNAc-O-(CH2)8COOCH3 | 37°C, pH 6.5 | Helicobacter pylori | |
| 26.12 | - |
Galbeta-(1-3)-GlcNAc-O-(CH2)8COOCH3 | 37°C, pH 6.5 | Helicobacter pylori | |
| 34.33 | - |
NeuAcalpha1-3Galbeta1-3GlcNAc-beta-O-(CH2)8COOCH3 | 37°C, pH 6.5 | Helicobacter pylori | |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
