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Literature summary for 2.4.1.64 extracted from
- Enzymatic properties and substrate specificity of the trehalose phosphorylase from Caldanaerobacter subterraneus (2011), Appl. Environ. Microbiol., 77, 6939-6944.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli XL10 Gold cells | Caldanaerobacter subterraneus subsp. pacificus |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| D-glucose | strong substrate inhibition, 500 mM D-glucose reduces the enzyme activity to 49.1% of the activity at the saturating concentration of 30 mM | Caldanaerobacter subterraneus subsp. pacificus |  |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 3.5 | - |
D-glucose | in 50 mM MES buffer (pH 6.0), at 60°C | Caldanaerobacter subterraneus subsp. pacificus | |
| 20.3 | - |
alpha-D-xylose | in 50 mM MES buffer (pH 6.0), at 60°C | Caldanaerobacter subterraneus subsp. pacificus | |
| 64.8 | - |
alpha-D-arabinose | in 50 mM MES buffer (pH 6.0), at 60°C | Caldanaerobacter subterraneus subsp. pacificus | |
| 171.5 | - |
alpha-D-galactose | in 50 mM MES buffer (pH 6.0), at 60°C | Caldanaerobacter subterraneus subsp. pacificus | |
| 183.4 | - |
alpha-L-fucose | in 50 mM MES buffer (pH 6.0), at 60°C | Caldanaerobacter subterraneus subsp. pacificus | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 90487 | - |
x * 90487, calculated from amino acid sequence | Caldanaerobacter subterraneus subsp. pacificus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Caldanaerobacter subterraneus subsp. pacificus | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| Ni-NTA agarose resin column chromatography | Caldanaerobacter subterraneus subsp. pacificus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| alpha,alpha-trehalose + phosphate | specific substrate | Caldanaerobacter subterraneus subsp. pacificus | beta-D-glucose 1-phosphate + D-glucose | - |
r | |
| beta-D-glucose 1-phosphate + alpha-D-allose | 4.5% activity compared to D-glucose | Caldanaerobacter subterraneus subsp. pacificus | ? | - |
r | |
| beta-D-glucose 1-phosphate + alpha-D-arabinose | 1.8% activity compared to D-glucose | Caldanaerobacter subterraneus subsp. pacificus | ? | - |
r | |
| beta-D-glucose 1-phosphate + alpha-D-fucose | 22% activity compared to D-glucose | Caldanaerobacter subterraneus subsp. pacificus | ? | - |
r | |
| beta-D-glucose 1-phosphate + alpha-D-galactose | 83.7% activity compared to D-glucose | Caldanaerobacter subterraneus subsp. pacificus | ? | - |
r | |
| beta-D-glucose 1-phosphate + alpha-D-lyxose | 1.3% activity compared to D-glucose | Caldanaerobacter subterraneus subsp. pacificus | ? | - |
r | |
| beta-D-glucose 1-phosphate + alpha-D-mannose | 14.1% activity compared to D-glucose | Caldanaerobacter subterraneus subsp. pacificus | ? | - |
r | |
| beta-D-glucose 1-phosphate + alpha-D-xylose | 135.2% activity compared to D-glucose | Caldanaerobacter subterraneus subsp. pacificus | ? | - |
r | |
| beta-D-glucose 1-phosphate + alpha-L-fucose | 109.2% activity compared to D-glucose | Caldanaerobacter subterraneus subsp. pacificus | ? | - |
r | |
| beta-D-glucose 1-phosphate + beta-L-arabinose | 79.9% activity compared to D-glucose | Caldanaerobacter subterraneus subsp. pacificus | ? | - |
r | |
| beta-D-glucose 1-phosphate + D-glucose | 100% activity with D-glucose | Caldanaerobacter subterraneus subsp. pacificus | alpha,alpha-trehalose + phosphate | - |
r | |
| additional information | the enzyme shows no measurable activity with alpha-D-glucosamine, alpha-D-ribose, D-maltose or D-kojibiose | Caldanaerobacter subterraneus subsp. pacificus | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 90487, calculated from amino acid sequence | Caldanaerobacter subterraneus subsp. pacificus |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| inverting trehalose phosphorylase | - |
Caldanaerobacter subterraneus subsp. pacificus |
| trehalose phosphorylase | - |
Caldanaerobacter subterraneus subsp. pacificus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 80 | - |
- |
Caldanaerobacter subterraneus subsp. pacificus |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 65 | - |
no loss of activity after 1 h of incubation at 65°C | Caldanaerobacter subterraneus subsp. pacificus |
| 72 | - |
50% residual activity remains after 1 h of incubation at 72°C | Caldanaerobacter subterraneus subsp. pacificus |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 51.4 | - |
alpha-D-arabinose | in 50 mM MES buffer (pH 6.0), at 60°C | Caldanaerobacter subterraneus subsp. pacificus | |
| 54 | - |
alpha-D-galactose | in 50 mM MES buffer (pH 6.0), at 60°C | Caldanaerobacter subterraneus subsp. pacificus | |
| 59 | - |
D-glucose | in 50 mM MES buffer (pH 6.0), at 60°C | Caldanaerobacter subterraneus subsp. pacificus | |
| 64.3 | - |
alpha-D-xylose | in 50 mM MES buffer (pH 6.0), at 60°C | Caldanaerobacter subterraneus subsp. pacificus | |
| 68.1 | - |
alpha-L-fucose | in 50 mM MES buffer (pH 6.0), at 60°C | Caldanaerobacter subterraneus subsp. pacificus | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 5.5 | - |
the pH optimum is slightly different for both reaction directions, with a lower optimum (about pH 5.5) for the synthesis reaction | Caldanaerobacter subterraneus subsp. pacificus |
| 7 | - |
the pH optimum is slightly different for both reaction directions, with a higher optimum (pH 7.0) for phosphorolysis | Caldanaerobacter subterraneus subsp. pacificus |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 4.5 | 7 | activity is over 90% in the range of pH 4.5 to 7.0 | Caldanaerobacter subterraneus subsp. pacificus |
pI Value
| Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|
| Caldanaerobacter subterraneus subsp. pacificus | calculated from amino acid sequence | - |
5.4 |
kcat/KM [mM/s]
| kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.315 | - |
alpha-D-galactose | in 50 mM MES buffer (pH 6.0), at 60°C | Caldanaerobacter subterraneus subsp. pacificus | |
| 0.371 | - |
alpha-L-fucose | in 50 mM MES buffer (pH 6.0), at 60°C | Caldanaerobacter subterraneus subsp. pacificus | |
| 0.793 | - |
alpha-D-arabinose | in 50 mM MES buffer (pH 6.0), at 60°C | Caldanaerobacter subterraneus subsp. pacificus | |
| 3.17 | - |
alpha-D-xylose | in 50 mM MES buffer (pH 6.0), at 60°C | Caldanaerobacter subterraneus subsp. pacificus | |
| 17 | - |
D-glucose | in 50 mM MES buffer (pH 6.0), at 60°C | Caldanaerobacter subterraneus subsp. pacificus | |
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