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Literature summary for 2.4.1.62 extracted from

  • Sprong, H.; Kruithof, B.; Leijendekker, R.; Slot, J.W.; Van Meer, G.; Van Der Sluijs, P.
    UDP-galactose:ceramide galactosyltransferase is a class I integral membrane protein of the endoplasmic reticulum (1998), J. Biol. Chem., 273, 25880-25888.
    View publication on PubMed
Search for more literature for 2.4.1.62

Cloned(Commentary)

Cloned (Comment) Organism
expression in HeLa cells, enzyme-deficient GM95 cells, CHO cells and CHOlec8 cells Rattus norvegicus
expression of enzyme fragments as His-tagged peptides in Escherichia coli Rattus norvegicus
in vitro transcription and translation of full length cDNA in presence of microsomes Rattus norvegicus

General Stability

General Stability Organism
half-life at 30°C: 4 h Rattus norvegicus

Localization

Localization Comment Organism GeneOntology No. Textmining
endoplasmic reticulum cisterna orientated towards the lumen Rattus norvegicus
-
-
Golgi apparatus not Rattus norvegicus 5794
-
microsome
-
 Rattus norvegicus
-
-
additional information not in the plasma membrane Rattus norvegicus
-
-
nuclear envelope
-
 Rattus norvegicus 5635
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
54000
-
 x * 54000, SDS-PAGE Rattus norvegicus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
UDPgalactose + N-acetyl-D-galactosaminyl-(N-acetylneuraminyl)-D-galactosyl-1,4-beta-D-glucosyl-N-acylsphingosine Rattus norvegicus
-
 UDP + D-galactosyl-1,3-beta-N-acetyl-D-galactosaminyl-(N-acetylneuraminyl)-D-galactosyl-D-glucosyl-N-acylsphingosine
-
 ?

Organism

Organism UniProt Comment Textmining
no activity in Mesocricetus auratus
-
-
-
Rattus norvegicus
-
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein Asn-linked high-mannose glycoprotein Rattus norvegicus
glycoprotein glycosylation at Asn 333 and Asn 78 Rattus norvegicus
additional information sensitive to cleavage by Endo H protease Rattus norvegicus

Source Tissue

Source Tissue Comment Organism Textmining
RT4-D6P2T cell oligodendrocyte cell line Rattus norvegicus
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
UDP-galactose + N-acetyl-D-galactosaminyl-(N-acetylneuraminyl)-D-galactosyl-1,4-beta-D-glucosyl-N-acylsphingosine
-
 Rattus norvegicus UDP + D-galactosyl-1,3-beta-N-acetyl-D-galactosaminyl-(N-acetylneuraminyl)-D-galactosyl-D-glucosyl-N-acylsphingosine
-
 ?
UDPgalactose + N-acetyl-D-galactosaminyl-(N-acetylneuraminyl)-D-galactosyl-1,4-beta-D-glucosyl-N-acylsphingosine
-
 Rattus norvegicus UDP + D-galactosyl-1,3-beta-N-acetyl-D-galactosaminyl-(N-acetylneuraminyl)-D-galactosyl-D-glucosyl-N-acylsphingosine
-
 ?

Subunits

Subunits Comment Organism
? x * 54000, SDS-PAGE Rattus norvegicus