Any feedback?
Literature summary for 2.4.1.58 extracted from
- Studies of phospholipid-requiring bacterial enzymes. III. Purification and properties of uridine diphosphate glucose:lipopolysaccharide glucosyltransferase I (1972), J. Biol. Chem., 247, 2614-2622.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| phosphatidylethanolamine | requirement, most effective phospholipid, phosphatidylethanolamine containing unsaturated or cyclopropane acyl groups is more effective than that containing saturated acyl groups | Salmonella enterica subsp. enterica serovar Typhimurium |  |
| Phospholipid | requirement | Salmonella enterica subsp. enterica serovar Typhimurium | |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| iodoacetamide | 5 mM, weak | Salmonella enterica subsp. enterica serovar Typhimurium | |
| additional information | not inhibited by p-chloromercuribenzoate, no detectable inhibition by non-substrate lipopolysaccharides obtained from other mutant strains of Salmonella typhimurium: G30G, G30A, G30, TV119, LT2 | Salmonella enterica subsp. enterica serovar Typhimurium |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.03 | - |
lipopolysaccharide | in presence of optimal concentrations of phosphatidylethanolamine | Salmonella enterica subsp. enterica serovar Typhimurium | |
| 0.1 | - |
lipopolysaccharide | in absence of phosphatidylethanolamine | Salmonella enterica subsp. enterica serovar Typhimurium | |
| 0.33 | - |
UDPglucose | - |
Salmonella enterica subsp. enterica serovar Typhimurium | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| membrane | - |
Salmonella enterica subsp. enterica serovar Typhimurium | 16020 | - |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Ba2+ | requires divalent cation, in decreasing order of effectiveness: Mg2+, Ba2+, Ca2+, Co2+, relative activities: Mg2+: 1.0, Ba2+: 0.77, Ca2+: 0.65, Co2+: 0.31 | Salmonella enterica subsp. enterica serovar Typhimurium | |
| Ca2+ | requires divalent cation, in decreasing order of effectiveness: Mg2+, Ba2+, Ca2+, Co2+, relative activities: Mg2+: 1.0, Ba2+: 0.77, Ca2+: 0.65, Co2+: 0.31 | Salmonella enterica subsp. enterica serovar Typhimurium | |
| Co2+ | requires divalent cation, in decreasing order of effectiveness: Mg2+, Ba2+, Ca2+, Co2+, relative activities: Mg2+: 1.0, Ba2+: 0.77, Ca2+: 0.65, Co2+: 0.31 | Salmonella enterica subsp. enterica serovar Typhimurium | |
| Mg2+ | requires divalent cation, in decreasing order of effectiveness: Mg2+, Ba2+, Ca2+, Co2+, relative activities: Mg2+: 1.0, Ba2+: 0.77, Ca2+: 0.65, Co2+: 0.31 | Salmonella enterica subsp. enterica serovar Typhimurium | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 20000 | - |
several peaks suggesting aggregation of enzyme, sucrose density gradient centrifugation | Salmonella enterica subsp. enterica serovar Typhimurium |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| UDPglucose + lipopolysaccharide | Salmonella enterica subsp. enterica serovar Typhimurium | addition of glucose during the biosynthesis of the lipopolysaccharide component of the cell envelope | UDP + D-glucosyllipopolysaccharide | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Salmonella enterica subsp. enterica serovar Typhimurium | - |
- |
- |
| Salmonella enterica subsp. enterica serovar Typhimurium G30A | - |
- |
- |
Posttranslational Modification
| Posttranslational Modification | Comment | Organism |
|---|---|---|
| additional information | no detectable phospholipid or lipopolysaccharide in the purified enzyme | Salmonella enterica subsp. enterica serovar Typhimurium |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Salmonella enterica subsp. enterica serovar Typhimurium |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
- |
Salmonella enterica subsp. enterica serovar Typhimurium |
Storage Stability
| Storage Stability | Organism |
|---|---|
| 4°C, 20% glycerol, for about 5 days: retains full activity, 10% loss of activity per week thereafter | Salmonella enterica subsp. enterica serovar Typhimurium |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | poor sugar donor: UDPgalactose, no sugar donors: TDPglucose, CDPglucose, ADPglucose, GDPmannose, glucose, glucose-1-phosphate, galactose, alpha-methylglucoside, beta-methylglucoside, no sugar acceptors: lipopolysaccharides from Salmonella typhimurium G30A, G30G, G30, TV119, LT2 | Salmonella enterica subsp. enterica serovar Typhimurium | ? | - |
? | |
| additional information | poor sugar donor: UDPgalactose, no sugar donors: TDPglucose, CDPglucose, ADPglucose, GDPmannose, glucose, glucose-1-phosphate, galactose, alpha-methylglucoside, beta-methylglucoside, no sugar acceptors: lipopolysaccharides from Salmonella typhimurium G30A, G30G, G30, TV119, LT2 | Salmonella enterica subsp. enterica serovar Typhimurium G30A | ? | - |
? | |
| UDPglucose + lipopolysaccharide | core region of the lipopolysaccharide | Salmonella enterica subsp. enterica serovar Typhimurium | UDP + D-glucosyllipopolysaccharide | - |
? | |
| UDPglucose + lipopolysaccharide | UDPglucose is the only effective glucosyl donor | Salmonella enterica subsp. enterica serovar Typhimurium | UDP + D-glucosyllipopolysaccharide | - |
? | |
| UDPglucose + lipopolysaccharide | glucose is transferred only to the glucose-deficient lipopolysaccharide obtained from Salmonella typhimurium SL1032, a strain lacking the glucosyl transferase system, transfers glucose to the L-glycero-D-mannoheptosyl residue, maximum incorporation of 0.5 mol glucose per mol of heptose | Salmonella enterica subsp. enterica serovar Typhimurium | UDP + D-glucosyllipopolysaccharide | - |
? | |
| UDPglucose + lipopolysaccharide | addition of glucose during the biosynthesis of the lipopolysaccharide component of the cell envelope | Salmonella enterica subsp. enterica serovar Typhimurium | UDP + D-glucosyllipopolysaccharide | - |
? | |
| UDPglucose + lipopolysaccharide | core region of the lipopolysaccharide | Salmonella enterica subsp. enterica serovar Typhimurium G30A | UDP + D-glucosyllipopolysaccharide | - |
? | |
| UDPglucose + lipopolysaccharide | UDPglucose is the only effective glucosyl donor | Salmonella enterica subsp. enterica serovar Typhimurium G30A | UDP + D-glucosyllipopolysaccharide | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 * 20000, additional faint bands: 44,5, 49 and 72 kDa, probably a single polypeptide chain, SDS-PAGE | Salmonella enterica subsp. enterica serovar Typhimurium |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Salmonella enterica subsp. enterica serovar Typhimurium |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | 8.5 | Tris-maleate or Tris-Cl buffers | Salmonella enterica subsp. enterica serovar Typhimurium |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
