BRENDA - Enzyme Database
show all sequences of 2.4.1.58

Studies of phospholipid-requiring bacterial enzymes. III. Purification and properties of uridine diphosphate glucose:lipopolysaccharide glucosyltransferase I

Muller, E.; Hinckley, A.; Rothfield, L.; J. Biol. Chem. 247, 2614-2622 (1972)

Data extracted from this reference:

Activating Compound
Activating Compound
Commentary
Organism
Structure
phosphatidylethanolamine
requirement, most effective phospholipid, phosphatidylethanolamine containing unsaturated or cyclopropane acyl groups is more effective than that containing saturated acyl groups
Salmonella enterica subsp. enterica serovar Typhimurium
Phospholipid
requirement
Salmonella enterica subsp. enterica serovar Typhimurium
Inhibitors
Inhibitors
Commentary
Organism
Structure
iodoacetamide
5 mM, weak
Salmonella enterica subsp. enterica serovar Typhimurium
additional information
not inhibited by p-chloromercuribenzoate, no detectable inhibition by non-substrate lipopolysaccharides obtained from other mutant strains of Salmonella typhimurium: G30G, G30A, G30, TV119, LT2
Salmonella enterica subsp. enterica serovar Typhimurium
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.03
-
lipopolysaccharide
in presence of optimal concentrations of phosphatidylethanolamine
Salmonella enterica subsp. enterica serovar Typhimurium
0.1
-
lipopolysaccharide
in absence of phosphatidylethanolamine
Salmonella enterica subsp. enterica serovar Typhimurium
0.33
-
UDPglucose
-
Salmonella enterica subsp. enterica serovar Typhimurium
Localization
Localization
Commentary
Organism
GeneOntology No.
Textmining
membrane
-
Salmonella enterica subsp. enterica serovar Typhimurium
16020
-
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
Ba2+
requires divalent cation, in decreasing order of effectiveness: Mg2+, Ba2+, Ca2+, Co2+, relative activities: Mg2+: 1.0, Ba2+: 0.77, Ca2+: 0.65, Co2+: 0.31
Salmonella enterica subsp. enterica serovar Typhimurium
Ca2+
requires divalent cation, in decreasing order of effectiveness: Mg2+, Ba2+, Ca2+, Co2+, relative activities: Mg2+: 1.0, Ba2+: 0.77, Ca2+: 0.65, Co2+: 0.31
Salmonella enterica subsp. enterica serovar Typhimurium
Co2+
requires divalent cation, in decreasing order of effectiveness: Mg2+, Ba2+, Ca2+, Co2+, relative activities: Mg2+: 1.0, Ba2+: 0.77, Ca2+: 0.65, Co2+: 0.31
Salmonella enterica subsp. enterica serovar Typhimurium
Mg2+
requires divalent cation, in decreasing order of effectiveness: Mg2+, Ba2+, Ca2+, Co2+, relative activities: Mg2+: 1.0, Ba2+: 0.77, Ca2+: 0.65, Co2+: 0.31
Salmonella enterica subsp. enterica serovar Typhimurium
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
20000
-
several peaks suggesting aggregation of enzyme, sucrose density gradient centrifugation
Salmonella enterica subsp. enterica serovar Typhimurium
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
UDPglucose + lipopolysaccharide
Salmonella enterica subsp. enterica serovar Typhimurium
addition of glucose during the biosynthesis of the lipopolysaccharide component of the cell envelope
UDP + D-glucosyllipopolysaccharide
-
-
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Salmonella enterica subsp. enterica serovar Typhimurium
-
-
-
Salmonella enterica subsp. enterica serovar Typhimurium G30A
-
-
-
Posttranslational Modification
Posttranslational Modification
Commentary
Organism
additional information
no detectable phospholipid or lipopolysaccharide in the purified enzyme
Salmonella enterica subsp. enterica serovar Typhimurium
Purification (Commentary)
Commentary
Organism
-
Salmonella enterica subsp. enterica serovar Typhimurium
Specific Activity [micromol/min/mg]
Specific Activity Minimum [mol/min/mg]
Specific Activity Maximum [mol/min/mg]
Commentary
Organism
additional information
-
-
Salmonella enterica subsp. enterica serovar Typhimurium
Storage Stability
Storage Stability
Organism
4C, 20% glycerol, for about 5 days: retains full activity, 10% loss of activity per week thereafter
Salmonella enterica subsp. enterica serovar Typhimurium
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
additional information
poor sugar donor: UDPgalactose, no sugar donors: TDPglucose, CDPglucose, ADPglucose, GDPmannose, glucose, glucose-1-phosphate, galactose, alpha-methylglucoside, beta-methylglucoside, no sugar acceptors: lipopolysaccharides from Salmonella typhimurium G30A, G30G, G30, TV119, LT2
489212
Salmonella enterica subsp. enterica serovar Typhimurium
?
-
-
-
-
additional information
poor sugar donor: UDPgalactose, no sugar donors: TDPglucose, CDPglucose, ADPglucose, GDPmannose, glucose, glucose-1-phosphate, galactose, alpha-methylglucoside, beta-methylglucoside, no sugar acceptors: lipopolysaccharides from Salmonella typhimurium G30A, G30G, G30, TV119, LT2
489212
Salmonella enterica subsp. enterica serovar Typhimurium G30A
?
-
-
-
-
additional information
poor sugar donor: UDPgalactose, no sugar donors: TDPglucose, CDPglucose, ADPglucose, GDPmannose, glucose, glucose-1-phosphate, galactose, alpha-methylglucoside, beta-methylglucoside, no sugar acceptors: lipopolysaccharides from Salmonella typhimurium G30A, G30G, G30, TV119, LT2
489212
Salmonella enterica subsp. enterica serovar Typhimurium LT2
?
-
-
-
-
UDPglucose + lipopolysaccharide
core region of the lipopolysaccharide
489212
Salmonella enterica subsp. enterica serovar Typhimurium
UDP + D-glucosyllipopolysaccharide
-
489212
Salmonella enterica subsp. enterica serovar Typhimurium
?
UDPglucose + lipopolysaccharide
UDPglucose is the only effective glucosyl donor
489212
Salmonella enterica subsp. enterica serovar Typhimurium
UDP + D-glucosyllipopolysaccharide
-
489212
Salmonella enterica subsp. enterica serovar Typhimurium
?
UDPglucose + lipopolysaccharide
glucose is transferred only to the glucose-deficient lipopolysaccharide obtained from Salmonella typhimurium SL1032, a strain lacking the glucosyl transferase system, transfers glucose to the L-glycero-D-mannoheptosyl residue, maximum incorporation of 0.5 mol glucose per mol of heptose
489212
Salmonella enterica subsp. enterica serovar Typhimurium
UDP + D-glucosyllipopolysaccharide
-
489212
Salmonella enterica subsp. enterica serovar Typhimurium
?
UDPglucose + lipopolysaccharide
addition of glucose during the biosynthesis of the lipopolysaccharide component of the cell envelope
489212
Salmonella enterica subsp. enterica serovar Typhimurium
UDP + D-glucosyllipopolysaccharide
-
-
-
?
UDPglucose + lipopolysaccharide
core region of the lipopolysaccharide
489212
Salmonella enterica subsp. enterica serovar Typhimurium G30A
UDP + D-glucosyllipopolysaccharide
-
489212
Salmonella enterica subsp. enterica serovar Typhimurium G30A
?
UDPglucose + lipopolysaccharide
UDPglucose is the only effective glucosyl donor
489212
Salmonella enterica subsp. enterica serovar Typhimurium G30A
UDP + D-glucosyllipopolysaccharide
-
489212
Salmonella enterica subsp. enterica serovar Typhimurium G30A
?
UDPglucose + lipopolysaccharide
core region of the lipopolysaccharide
489212
Salmonella enterica subsp. enterica serovar Typhimurium LT2
UDP + D-glucosyllipopolysaccharide
-
489212
Salmonella enterica subsp. enterica serovar Typhimurium LT2
?
UDPglucose + lipopolysaccharide
UDPglucose is the only effective glucosyl donor
489212
Salmonella enterica subsp. enterica serovar Typhimurium LT2
UDP + D-glucosyllipopolysaccharide
-
489212
Salmonella enterica subsp. enterica serovar Typhimurium LT2
?
Subunits
Subunits
Commentary
Organism
monomer
1 * 20000, additional faint bands: 44,5, 49 and 72 kDa, probably a single polypeptide chain, SDS-PAGE
Salmonella enterica subsp. enterica serovar Typhimurium
Temperature Optimum [C]
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
37
-
assay at
Salmonella enterica subsp. enterica serovar Typhimurium
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.5
8.5
Tris-maleate or Tris-Cl buffers
Salmonella enterica subsp. enterica serovar Typhimurium
Activating Compound (protein specific)
Activating Compound
Commentary
Organism
Structure
phosphatidylethanolamine
requirement, most effective phospholipid, phosphatidylethanolamine containing unsaturated or cyclopropane acyl groups is more effective than that containing saturated acyl groups
Salmonella enterica subsp. enterica serovar Typhimurium
Phospholipid
requirement
Salmonella enterica subsp. enterica serovar Typhimurium
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
iodoacetamide
5 mM, weak
Salmonella enterica subsp. enterica serovar Typhimurium
additional information
not inhibited by p-chloromercuribenzoate, no detectable inhibition by non-substrate lipopolysaccharides obtained from other mutant strains of Salmonella typhimurium: G30G, G30A, G30, TV119, LT2
Salmonella enterica subsp. enterica serovar Typhimurium
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.03
-
lipopolysaccharide
in presence of optimal concentrations of phosphatidylethanolamine
Salmonella enterica subsp. enterica serovar Typhimurium
0.1
-
lipopolysaccharide
in absence of phosphatidylethanolamine
Salmonella enterica subsp. enterica serovar Typhimurium
0.33
-
UDPglucose
-
Salmonella enterica subsp. enterica serovar Typhimurium
Localization (protein specific)
Localization
Commentary
Organism
GeneOntology No.
Textmining
membrane
-
Salmonella enterica subsp. enterica serovar Typhimurium
16020
-
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
Ba2+
requires divalent cation, in decreasing order of effectiveness: Mg2+, Ba2+, Ca2+, Co2+, relative activities: Mg2+: 1.0, Ba2+: 0.77, Ca2+: 0.65, Co2+: 0.31
Salmonella enterica subsp. enterica serovar Typhimurium
Ca2+
requires divalent cation, in decreasing order of effectiveness: Mg2+, Ba2+, Ca2+, Co2+, relative activities: Mg2+: 1.0, Ba2+: 0.77, Ca2+: 0.65, Co2+: 0.31
Salmonella enterica subsp. enterica serovar Typhimurium
Co2+
requires divalent cation, in decreasing order of effectiveness: Mg2+, Ba2+, Ca2+, Co2+, relative activities: Mg2+: 1.0, Ba2+: 0.77, Ca2+: 0.65, Co2+: 0.31
Salmonella enterica subsp. enterica serovar Typhimurium
Mg2+
requires divalent cation, in decreasing order of effectiveness: Mg2+, Ba2+, Ca2+, Co2+, relative activities: Mg2+: 1.0, Ba2+: 0.77, Ca2+: 0.65, Co2+: 0.31
Salmonella enterica subsp. enterica serovar Typhimurium
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
20000
-
several peaks suggesting aggregation of enzyme, sucrose density gradient centrifugation
Salmonella enterica subsp. enterica serovar Typhimurium
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
UDPglucose + lipopolysaccharide
Salmonella enterica subsp. enterica serovar Typhimurium
addition of glucose during the biosynthesis of the lipopolysaccharide component of the cell envelope
UDP + D-glucosyllipopolysaccharide
-
-
?
Posttranslational Modification (protein specific)
Posttranslational Modification
Commentary
Organism
additional information
no detectable phospholipid or lipopolysaccharide in the purified enzyme
Salmonella enterica subsp. enterica serovar Typhimurium
Purification (Commentary) (protein specific)
Commentary
Organism
-
Salmonella enterica subsp. enterica serovar Typhimurium
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [mol/min/mg]
Specific Activity Maximum [mol/min/mg]
Commentary
Organism
additional information
-
-
Salmonella enterica subsp. enterica serovar Typhimurium
Storage Stability (protein specific)
Storage Stability
Organism
4C, 20% glycerol, for about 5 days: retains full activity, 10% loss of activity per week thereafter
Salmonella enterica subsp. enterica serovar Typhimurium
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
additional information
poor sugar donor: UDPgalactose, no sugar donors: TDPglucose, CDPglucose, ADPglucose, GDPmannose, glucose, glucose-1-phosphate, galactose, alpha-methylglucoside, beta-methylglucoside, no sugar acceptors: lipopolysaccharides from Salmonella typhimurium G30A, G30G, G30, TV119, LT2
489212
Salmonella enterica subsp. enterica serovar Typhimurium
?
-
-
-
-
additional information
poor sugar donor: UDPgalactose, no sugar donors: TDPglucose, CDPglucose, ADPglucose, GDPmannose, glucose, glucose-1-phosphate, galactose, alpha-methylglucoside, beta-methylglucoside, no sugar acceptors: lipopolysaccharides from Salmonella typhimurium G30A, G30G, G30, TV119, LT2
489212
Salmonella enterica subsp. enterica serovar Typhimurium G30A
?
-
-
-
-
additional information
poor sugar donor: UDPgalactose, no sugar donors: TDPglucose, CDPglucose, ADPglucose, GDPmannose, glucose, glucose-1-phosphate, galactose, alpha-methylglucoside, beta-methylglucoside, no sugar acceptors: lipopolysaccharides from Salmonella typhimurium G30A, G30G, G30, TV119, LT2
489212
Salmonella enterica subsp. enterica serovar Typhimurium LT2
?
-
-
-
-
UDPglucose + lipopolysaccharide
core region of the lipopolysaccharide
489212
Salmonella enterica subsp. enterica serovar Typhimurium
UDP + D-glucosyllipopolysaccharide
-
489212
Salmonella enterica subsp. enterica serovar Typhimurium
?
UDPglucose + lipopolysaccharide
UDPglucose is the only effective glucosyl donor
489212
Salmonella enterica subsp. enterica serovar Typhimurium
UDP + D-glucosyllipopolysaccharide
-
489212
Salmonella enterica subsp. enterica serovar Typhimurium
?
UDPglucose + lipopolysaccharide
glucose is transferred only to the glucose-deficient lipopolysaccharide obtained from Salmonella typhimurium SL1032, a strain lacking the glucosyl transferase system, transfers glucose to the L-glycero-D-mannoheptosyl residue, maximum incorporation of 0.5 mol glucose per mol of heptose
489212
Salmonella enterica subsp. enterica serovar Typhimurium
UDP + D-glucosyllipopolysaccharide
-
489212
Salmonella enterica subsp. enterica serovar Typhimurium
?
UDPglucose + lipopolysaccharide
addition of glucose during the biosynthesis of the lipopolysaccharide component of the cell envelope
489212
Salmonella enterica subsp. enterica serovar Typhimurium
UDP + D-glucosyllipopolysaccharide
-
-
-
?
UDPglucose + lipopolysaccharide
core region of the lipopolysaccharide
489212
Salmonella enterica subsp. enterica serovar Typhimurium G30A
UDP + D-glucosyllipopolysaccharide
-
489212
Salmonella enterica subsp. enterica serovar Typhimurium G30A
?
UDPglucose + lipopolysaccharide
UDPglucose is the only effective glucosyl donor
489212
Salmonella enterica subsp. enterica serovar Typhimurium G30A
UDP + D-glucosyllipopolysaccharide
-
489212
Salmonella enterica subsp. enterica serovar Typhimurium G30A
?
UDPglucose + lipopolysaccharide
core region of the lipopolysaccharide
489212
Salmonella enterica subsp. enterica serovar Typhimurium LT2
UDP + D-glucosyllipopolysaccharide
-
489212
Salmonella enterica subsp. enterica serovar Typhimurium LT2
?
UDPglucose + lipopolysaccharide
UDPglucose is the only effective glucosyl donor
489212
Salmonella enterica subsp. enterica serovar Typhimurium LT2
UDP + D-glucosyllipopolysaccharide
-
489212
Salmonella enterica subsp. enterica serovar Typhimurium LT2
?
Subunits (protein specific)
Subunits
Commentary
Organism
monomer
1 * 20000, additional faint bands: 44,5, 49 and 72 kDa, probably a single polypeptide chain, SDS-PAGE
Salmonella enterica subsp. enterica serovar Typhimurium
Temperature Optimum [C] (protein specific)
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
37
-
assay at
Salmonella enterica subsp. enterica serovar Typhimurium
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.5
8.5
Tris-maleate or Tris-Cl buffers
Salmonella enterica subsp. enterica serovar Typhimurium
Other publictions for EC 2.4.1.58
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [C]
Temperature Range [C]
Temperature Stability [C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [C] (protein specific)
Temperature Range [C] (protein specific)
Temperature Stability [C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
719223
Twelkmeyer
The role of lic2B in lipopolys ...
Haemophilus influenzae, Haemophilus influenzae Eagan
Carbohydr. Res.
346
1262-1266
2011
-
-
1
-
-
-
-
-
-
-
-
-
-
3
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
720277
Perumal
Cloning and targeted disruptio ...
Pseudomonas aeruginosa
J. Mol. Microbiol. Biotechnol.
19
169-179
2010
-
-
1
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
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-
-
-
-
-
-
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-
1
-
-
-
-
-
-
-
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-
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-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
1
-
-
-
703886
Schwingel
A unique glycosyltransferase i ...
Moraxella catarrhalis
Glycobiology
18
447-455
2008
-
-
1
-
1
-
-
-
-
-
-
-
-
6
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
3
-
-
3
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
3
-
-
-
489300
Creeger
Cloning of genes for bacterial ...
Escherichia coli, Escherichia coli K12 CS520, Salmonella enterica subsp. enterica serovar Typhimurium
J. Biol. Chem.
254
804-810
1979
2
-
1
-
-
-
-
2
2
1
-
2
-
8
-
-
1
-
-
-
2
-
15
-
2
-
-
-
-
-
-
-
-
-
-
2
-
1
-
-
-
-
-
-
-
2
2
1
-
2
-
-
-
1
-
-
2
-
15
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
489212
Muller
Studies of phospholipid-requir ...
Salmonella enterica subsp. enterica serovar Typhimurium, Salmonella enterica subsp. enterica serovar Typhimurium G30A
J. Biol. Chem.
247
2614-2622
1972
2
-
-
-
-
-
2
3
1
4
1
1
-
2
-
1
1
-
-
-
1
1
11
1
1
-
-
-
1
-
-
-
-
-
-
2
-
-
-
-
-
-
-
2
-
3
1
4
1
1
-
-
1
1
-
-
1
1
11
1
1
-
-
-
1
-
-
-
-
-
-
-
-
-
489298
Rothfield
Biosynthesis of bacterial lipo ...
Salmonella enterica subsp. enterica serovar Typhimurium, Salmonella enterica subsp. enterica serovar Typhimurium SL797
J. Biol. Chem.
239
2788-2795
1964
-
-
-
-
-
1
-
2
1
2
-
2
-
2
-
-
-
-
-
-
1
1
10
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
2
1
2
-
2
-
-
-
-
-
-
1
1
10
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-