BRENDA - Enzyme Database
show all sequences of 2.4.1.54

Studies on the biosynthesis of mannan in Micrococcus lysodeikticus. II. The enzymatic synthesis of mannosyl-l-phosphoryl-undecaprenol

Lahav, M.; Chiu, T.H.; Lennarz, W.J.; J. Biol. Chem. 244, 5890-5898 (1969)

Data extracted from this reference:

Activating Compound
Activating Compound
Commentary
Organism
Structure
Cutscum
activates
Micrococcus luteus
Detergents
activate
Micrococcus luteus
Nonidet P-40
activates
Micrococcus luteus
phosphatidylethanolamine
activates
Micrococcus luteus
phosphatidylglycerol
requirement
Micrococcus luteus
sodium lauryl sulfate
activates
Micrococcus luteus
Triton X-100
activates
Micrococcus luteus
Inhibitors
Inhibitors
Commentary
Organism
Structure
GDP
-
Micrococcus luteus
phosphatidylglycerol
inhibits in excess amount
Micrococcus luteus
undecaprenyl phosphate
inhibits in excess amount
Micrococcus luteus
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
Ca2+
activation, can replace Mg2+ to some extent
Micrococcus luteus
Mg2+
optimal concentration: 67 mM; requirement
Micrococcus luteus
Mn2+
activation, can replace Mg2+ to some extent
Micrococcus luteus
additional information
not activated by Co2+
Micrococcus luteus
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
additional information
Micrococcus luteus
involved in biosynthesis of mannan
?
-
-
-
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Micrococcus luteus
-
Micrococcus lysodeikticus
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
GDPmannose + ficaprenyl phosphate
effective substrate
489288
Micrococcus luteus
GDP + mannosyl-1-phosphorylficaprenol
-
489288
Micrococcus luteus
?
GDPmannose + undecaprenyl phosphate
-
489288
Micrococcus luteus
GDP + D-mannosyl-1-phosphoundecaprenol
-
489288
Micrococcus luteus
r
additional information
involved in biosynthesis of mannan
489288
Micrococcus luteus
?
-
-
-
-
Temperature Optimum [°C]
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
37
-
assay at
Micrococcus luteus
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
6.9
7.5
0.06 M Tris-maleate buffer
Micrococcus luteus
pH Range
pH Minimum
pH Maximum
Commentary
Organism
6.2
8.6
about half-maximal activity at pH 6.2 and 8.6
Micrococcus luteus
Activating Compound (protein specific)
Activating Compound
Commentary
Organism
Structure
Cutscum
activates
Micrococcus luteus
Detergents
activate
Micrococcus luteus
Nonidet P-40
activates
Micrococcus luteus
phosphatidylethanolamine
activates
Micrococcus luteus
phosphatidylglycerol
requirement
Micrococcus luteus
sodium lauryl sulfate
activates
Micrococcus luteus
Triton X-100
activates
Micrococcus luteus
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
GDP
-
Micrococcus luteus
phosphatidylglycerol
inhibits in excess amount
Micrococcus luteus
undecaprenyl phosphate
inhibits in excess amount
Micrococcus luteus
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
Ca2+
activation, can replace Mg2+ to some extent
Micrococcus luteus
Mg2+
optimal concentration: 67 mM; requirement
Micrococcus luteus
Mn2+
activation, can replace Mg2+ to some extent
Micrococcus luteus
additional information
not activated by Co2+
Micrococcus luteus
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
additional information
Micrococcus luteus
involved in biosynthesis of mannan
?
-
-
-
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
GDPmannose + ficaprenyl phosphate
effective substrate
489288
Micrococcus luteus
GDP + mannosyl-1-phosphorylficaprenol
-
489288
Micrococcus luteus
?
GDPmannose + undecaprenyl phosphate
-
489288
Micrococcus luteus
GDP + D-mannosyl-1-phosphoundecaprenol
-
489288
Micrococcus luteus
r
additional information
involved in biosynthesis of mannan
489288
Micrococcus luteus
?
-
-
-
-
Temperature Optimum [°C] (protein specific)
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
37
-
assay at
Micrococcus luteus
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
6.9
7.5
0.06 M Tris-maleate buffer
Micrococcus luteus
pH Range (protein specific)
pH Minimum
pH Maximum
Commentary
Organism
6.2
8.6
about half-maximal activity at pH 6.2 and 8.6
Micrococcus luteus
Other publictions for EC 2.4.1.54
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
681770
Rush
Partial purification of mannos ...
Micrococcus luteus
Methods Mol. Biol.
347
13-30
2006
-
1
-
-
-
-
-
-
-
-
1
-
-
2
-
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
1
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
489291
Rush
Mannosylphosphoryldolichol-med ...
Micrococcus luteus
J. Biol. Chem.
268
13110-13117
1993
-
-
-
-
-
-
-
-
1
-
-
1
-
1
-
-
1
-
-
-
-
1
5
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
1
-
-
-
1
-
-
-
1
5
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
489289
Clark
An artificial mannosyl accepto ...
Vigna radiata var. radiata
FEBS Lett.
32
84-86
1973
1
-
-
-
-
-
-
-
1
-
-
-
-
1
-
-
-
-
-
1
-
-
3
-
1
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
3
-
1
-
1
-
-
-
-
-
-
-
-
-
-
-
489290
Forsee
Biosynthesis of mannosyl- and ...
Gossypium hirsutum, Mycolicibacterium smegmatis
J. Biol. Chem.
248
2858-2867
1973
-
-
-
-
-
-
1
2
2
2
-
1
-
2
-
-
-
-
-
1
-
-
6
-
1
-
-
-
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
2
2
2
-
1
-
-
-
-
-
1
-
-
6
-
1
-
-
-
1
1
-
-
-
-
-
-
-
-
489288
Lahav
Studies on the biosynthesis of ...
Micrococcus luteus
J. Biol. Chem.
244
5890-5898
1969
7
-
-
-
-
-
3
-
-
4
-
1
-
1
-
-
-
-
-
-
-
-
3
-
1
-
-
-
1
1
-
-
-
-
-
7
-
-
-
-
-
-
-
3
-
-
-
4
-
1
-
-
-
-
-
-
-
-
3
-
1
-
-
-
1
1
-
-
-
-
-
-
-
-