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Literature summary for 2.4.1.50 extracted from
- Characterization of membrane-bound collagen galactosyltransferase of human blood platelets (1971), Biochim. Biophys. Acta, 252, 546-552.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| Triton X-100 | 0.1%, 2fold stimulation | Homo sapiens |  |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Cu2+ | - |
Homo sapiens | |
| additional information | not inhibited by Hg2+, acetylsalicylic acid, D-glucosamine, glutathione, ADP | Homo sapiens | |
| p-hydroxymercuribenzoate | 10 mM, 50% inhibition | Homo sapiens | |
| Pb2+ | - |
Homo sapiens | |
| UDP | - |
Homo sapiens | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| plasma membrane | blood platelet plasma membrane, may be buried in the interior of the membrane or located on its inner surface | Homo sapiens | 5886 | - |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | 20% of activation by Mg2+ | Homo sapiens | |
| Cd2+ | 20% of activation by Mg2+ | Homo sapiens | |
| Co2+ | 50% of activation by Mg2+ | Homo sapiens | |
| Mg2+ | 50% of activation by Mg2+ | Homo sapiens | |
| Mn2+ | requirement | Homo sapiens | |
| Mn2+ | optimal concentration: 15 mM | Homo sapiens | |
| additional information | not activated by Cu2+ | Homo sapiens | |
| additional information | not activated by Zn2+ | Homo sapiens | |
| additional information | not activated by Hg2+ | Homo sapiens | |
| additional information | not activated by K+, Pb2+ | Homo sapiens | |
| additional information | not activated by Fe3+, Ba2+ | Homo sapiens |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| UDPgalactose + procollagen 5-hydroxy-L-lysine | Homo sapiens | incorporation of monosaccharide precursors into the heterosaccharide chains of collagen | UDP + procollagen 5-(D-galactosyloxy)-L-lysine | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Homo sapiens | - |
- |
- |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| blood platelet | blood platelet plasma membranes | Homo sapiens | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
- |
Homo sapiens |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | no sugar donors: ADP-glucose, UDP-N-acetylglucosamine, UDP-N-acetylgalactosamine | Homo sapiens | ? | - |
? | |
| additional information | no sugar donor: UDPglucose | Homo sapiens | ? | - |
? | |
| additional information | no acceptors: transferrin, native fetuin | Homo sapiens | ? | - |
? | |
| UDPgalactose + fetuin with sialic acid and galactose removed | 33% of activity with bovine achilles tendon collagen with glucose and galactose residues removed | Homo sapiens | ? | - |
? | |
| UDPgalactose + ovalbumin | 17.9% of activity with bovine achilles tendon collagen with glucose and galactose residues removed | Homo sapiens | ? | - |
? | |
| UDPgalactose + procollagen 5-hydroxy-L-lysine | bovine achilles tendon collagen with glucose and galactose residues removed | Homo sapiens | UDP + procollagen 5-(D-galactosyloxy)-L-lysine | - |
? | |
| UDPgalactose + procollagen 5-hydroxy-L-lysine | high specificity for specific hydroxylysine residues in collagen | Homo sapiens | UDP + procollagen 5-(D-galactosyloxy)-L-lysine | - |
? | |
| UDPgalactose + procollagen 5-hydroxy-L-lysine | incorporation of monosaccharide precursors into the heterosaccharide chains of collagen | Homo sapiens | UDP + procollagen 5-(D-galactosyloxy)-L-lysine | - |
? | |
| UDPgalactose + submaxillary glycoprotein with sialic acid removed | bovine submaxillary glycoprotein with sialic acid removed, 9.5% of activity with achilles tendon collagen with glucose and galactose residues removed | Homo sapiens | ? | - |
? | |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Homo sapiens |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | - |
- |
Homo sapiens |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 5.5 | 9.5 | about half-maximal activity at pH 5.5 and 9.5 | Homo sapiens |
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