Application | Comment | Organism |
---|---|---|
food industry | the enzyme catalzes the in vitro synthesis of prebiotic oligosaccharides in mango and pineapple juices. Sucrose content of the juices is eliminated resulting in its lower calorific value. Potential of dextransucrase for production of functional foods | Weissella cibaria |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
CoCl2 | 1 mM, 29% decrease of activity | Weissella cibaria | |
EDTA | 0.5 mM, 31% decrease of activity | Weissella cibaria | |
MnSO4 | 1 mM, 22% decrease of activity | Weissella cibaria | |
NiSO4 | 1 mM, 36% decrease of activity | Weissella cibaria | |
Urea | - |
Weissella cibaria |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
19.2 | - |
sucrose | pH 5.4, 40°C | Weissella cibaria |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
extracellular | - |
Weissella cibaria | - |
- |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | 3 mM, enhances activity by 25% | Weissella cibaria | |
Mg2+ | 0.6 mM, enhances activity by 40% | Weissella cibaria |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
180000 | - |
SDS-PAGE | Weissella cibaria |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Weissella cibaria | - |
- |
- |
Weissella cibaria RBA12 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
25fold purification by PEG-400 and PEG-1500 fractionation, followed by gel filtration | Weissella cibaria |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
sucrose + D-glucose | isomalto-oligosaccharides from DP3 to DP5 along with isomaltose 44 (DP2) and leucrose (DP2) are synthesized | Weissella cibaria | D-fructose + isomalto-oligosaccharide + isomaltose + leucrose | - |
? | |
sucrose + D-glucose | isomalto-oligosaccharides from DP3 to DP5 along with isomaltose 44 (DP2) and leucrose (DP2) are synthesized | Weissella cibaria RBA12 | D-fructose + isomalto-oligosaccharide + isomaltose + leucrose | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 180000, SDS-PAGE | Weissella cibaria |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | - |
- |
Weissella cibaria |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
1 h, pH 5.4, stable | Weissella cibaria |
40 | - |
1 h, pH 5.4, 8% loss of activity | Weissella cibaria |
60 | - |
1 h, pH 5.4, complete loss of activity | Weissella cibaria |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5.4 | - |
- |
Weissella cibaria |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
4.5 | - |
1 h, 32% loss of activity | Weissella cibaria |
5 | - |
1 h, 12% loss of activity | Weissella cibaria |
5.5 | - |
1 h, 7% loss of activity | Weissella cibaria |
6 | - |
1 h, 17% loss of activity | Weissella cibaria |