Cloned (Comment) | Organism |
---|---|
enzyme expression in the periplasmic space of seven different Escherichia coli strains with inducer molecules such as lactose or IPTG. Escherichia coli strain BL21-CodonPlus(DE3)-RIL exhibits the highest enzyme activity with lactose, method optimization with respect to culture conditions results in a 12fold increase in activity at 17°C and 7.5 mM lactose | Leuconostoc citreum |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
160000 | - |
x * 160000, recombinant enzyme, SDS-PAGE, x * 167000, about, sequence calculation | Leuconostoc citreum |
167000 | - |
x * 160000, recombinant enzyme, SDS-PAGE, x * 167000, about, sequence calculation | Leuconostoc citreum |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Leuconostoc citreum | DexT catalyzes the polymerization of sucrose to dextran via a transglucosyl reaction | ? | - |
? | |
additional information | Leuconostoc citreum KM20 | DexT catalyzes the polymerization of sucrose to dextran via a transglucosyl reaction | ? | - |
? | |
sucrose + [(1->6)-alpha-D-glucosyl]n | Leuconostoc citreum | - |
D-fructose + [(1->6)-alpha-D-glucosyl]n+1 | - |
? | |
sucrose + [(1->6)-alpha-D-glucosyl]n | Leuconostoc citreum KM20 | - |
D-fructose + [(1->6)-alpha-D-glucosyl]n+1 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Leuconostoc citreum | B1N0B6 | - |
- |
Leuconostoc citreum KM20 | B1N0B6 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | DexT catalyzes the polymerization of sucrose to dextran via a transglucosyl reaction | Leuconostoc citreum | ? | - |
? | |
additional information | DexT catalyzes the polymerization of sucrose to dextran via a transglucosyl reaction | Leuconostoc citreum KM20 | ? | - |
? | |
sucrose + [(1->6)-alpha-D-glucosyl]n | - |
Leuconostoc citreum | D-fructose + [(1->6)-alpha-D-glucosyl]n+1 | - |
? | |
sucrose + [(1->6)-alpha-D-glucosyl]n | substrate is dextran | Leuconostoc citreum | D-fructose + [(1->6)-alpha-D-glucosyl]n+1 | product analysis by thin layer chromatography | ? | |
sucrose + [(1->6)-alpha-D-glucosyl]n | - |
Leuconostoc citreum KM20 | D-fructose + [(1->6)-alpha-D-glucosyl]n+1 | - |
? | |
sucrose + [(1->6)-alpha-D-glucosyl]n | substrate is dextran | Leuconostoc citreum KM20 | D-fructose + [(1->6)-alpha-D-glucosyl]n+1 | product analysis by thin layer chromatography | ? |
Subunits | Comment | Organism |
---|---|---|
? | x * 160000, recombinant enzyme, SDS-PAGE, x * 167000, about, sequence calculation | Leuconostoc citreum |
Synonyms | Comment | Organism |
---|---|---|
DexT | - |
Leuconostoc citreum |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
recombinant enzyme | Leuconostoc citreum |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
35 | - |
purified recombinant enzyme, stable up to | Leuconostoc citreum |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5.5 | - |
recombinant enzyme | Leuconostoc citreum |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
4 | 7.5 | purified recombinant enzyme, stable | Leuconostoc citreum |
General Information | Comment | Organism |
---|---|---|
evolution | dextransucrase belongs to the glycohydrolase family GH 70 | Leuconostoc citreum |