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Literature summary for 2.4.1.5 extracted from

  • Vidal, R.F.; Martinez, A.; Moulis, C.; Escalier, P.; Morel, S.; Remaud-Simeon, M.; Monsan, P.
    A novel dextransucrase is produced by Leuconostoc citreum strain B/110-1-2: an isolate used for the industrial production of dextran and dextran derivatives (2011), J. Ind. Microbiol. Biotechnol., 38, 1499-1506.
    View publication on PubMed
Search for more literature for 2.4.1.5

Application

Application Comment Organism
synthesis the strain Leuconostoc citreum strain B/110-1-2 is used for industrial production of dextran and dextran derivatives Leuconostoc citreum

Cloned(Commentary)

Cloned (Comment) Organism
two ORFs coding for strain B/110-1-2 dextransucrases, DNA and amino acid sequence determination and analysis, sequence comparisons Leuconostoc citreum

Localization

Localization Comment Organism GeneOntology No. Textmining
extracellular
-
 Leuconostoc citreum
-
-
intracellular
-
 Leuconostoc citreum 5622
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
160000 180000 intracellular dextransucrase 1 and extracellular dextransucrase Leuconostoc citreum
220000 240000 intracellular dextransucrse 2 Leuconostoc citreum

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Leuconostoc citreum structural analysis, by HPAEC-PAD, HPSEC, and 13C-NMR, of the polymer and oligodextrans produced by the B/110-1-2 dextransucrases, overview ?
-
 ?
additional information Leuconostoc citreum B/110-1-2 structural analysis, by HPAEC-PAD, HPSEC, and 13C-NMR, of the polymer and oligodextrans produced by the B/110-1-2 dextransucrases, overview ?
-
 ?
sucrose + [(1->6)-alpha-D-glucosyl]n Leuconostoc citreum
-
 D-fructose + [(1->6)-alpha-D-glucosyl]n+1
-
 ?
sucrose + [(1->6)-alpha-D-glucosyl]n Leuconostoc citreum B/110-1-2
-
 D-fructose + [(1->6)-alpha-D-glucosyl]n+1
-
 ?

Organism

Organism UniProt Comment Textmining
Leuconostoc citreum D5FS20 isolated from sugarcane juice
-
Leuconostoc citreum B/110-1-2 D5FS20 isolated from sugarcane juice
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information structural analysis, by HPAEC-PAD, HPSEC, and 13C-NMR, of the polymer and oligodextrans produced by the B/110-1-2 dextransucrases, overview Leuconostoc citreum ?
-
 ?
additional information structural analysis, by HPAEC-PAD, HPSEC, and 13C-NMR, of the polymer and oligodextrans produced by the B/110-1-2 dextransucrases, overview Leuconostoc citreum B/110-1-2 ?
-
 ?
sucrose + [(1->6)-alpha-D-glucosyl]n
-
 Leuconostoc citreum D-fructose + [(1->6)-alpha-D-glucosyl]n+1
-
 ?
sucrose + [(1->6)-alpha-D-glucosyl]n
-
 Leuconostoc citreum B/110-1-2 D-fructose + [(1->6)-alpha-D-glucosyl]n+1
-
 ?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
 assay at Leuconostoc citreum

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5.4
-
 assay at Leuconostoc citreum

General Information

General Information Comment Organism
physiological function in presence of sucrose, the strain B/110-1-2 produces two cell-associated dextransucrases (31.54% of the total glucosyltransferase activity) with molecular weights of 160 and 240 kDa and a soluble dextransucrase (68.46% of the total glucosyltransferase activity) at 160-180 kDa Leuconostoc citreum