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Literature summary for 2.4.1.5 extracted from

  • Rabelo, M.C.; Fontes, C.M.; Rodrigues, S.
    Stability Study of Crude Dextransucrase from Leuconostoc citreum NRRL B-742 (2011), Indian J. Microbiol., 51, 164-170.
    View publication on PubMedView publication on EuropePMC

General Stability

General Stability Organism
stability of crude dextransucrase from Leuconostoc citreum strain B-742 in synthetic and in cashew apple juice culture broth, evaluation, overview Leuconostoc citreum

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ required Leuconostoc citreum

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Leuconostoc citreum dextransucrase promotes the sucrose cleavage polymerizing the glucose into dextran chain and releasing the fructose moiety ?
-
?
additional information Leuconostoc citreum NRRL B-742 dextransucrase promotes the sucrose cleavage polymerizing the glucose into dextran chain and releasing the fructose moiety ?
-
?
sucrose + [(1->6)-alpha-D-glucosyl]n Leuconostoc citreum
-
D-fructose + [(1->6)-alpha-D-glucosyl]n+1
-
?
sucrose + [(1->6)-alpha-D-glucosyl]n Leuconostoc citreum NRRL B-742
-
D-fructose + [(1->6)-alpha-D-glucosyl]n+1
-
?

Organism

Organism UniProt Comment Textmining
Leuconostoc citreum
-
formerly Leuconostoc mesenteroides B-742
-
Leuconostoc citreum NRRL B-742
-
formerly Leuconostoc mesenteroides B-742
-

Source Tissue

Source Tissue Comment Organism Textmining
cell culture the enzyme production and activity is highest at pH 6.5 and 25°C, high stability at room temperature of 30°C, condition evaluation, overview Leuconostoc citreum
-

Storage Stability

Storage Stability Organism
25°C, crude enzyme in cashew apple juice, pH 6.5, 48 h , quite stable Leuconostoc citreum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information dextransucrase promotes the sucrose cleavage polymerizing the glucose into dextran chain and releasing the fructose moiety Leuconostoc citreum ?
-
?
additional information dextransucrase promotes the sucrose cleavage polymerizing the glucose into dextran chain and releasing the fructose moiety Leuconostoc citreum NRRL B-742 ?
-
?
sucrose + [(1->6)-alpha-D-glucosyl]n
-
Leuconostoc citreum D-fructose + [(1->6)-alpha-D-glucosyl]n+1
-
?
sucrose + [(1->6)-alpha-D-glucosyl]n
-
Leuconostoc citreum NRRL B-742 D-fructose + [(1->6)-alpha-D-glucosyl]n+1
-
?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
assay at Leuconostoc citreum

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
25 35 activity range analyzed Leuconostoc citreum

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.5
-
assay at Leuconostoc citreum

pH Range

pH Minimum pH Maximum Comment Organism
4.5 6.5 activity range analyzed Leuconostoc citreum

Expression

Organism Comment Expression
Leuconostoc citreum the enzyme is induced by sucrose up