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Literature summary for 2.4.1.5 extracted from

  • Ryu, H.; Jin, X.; Lee, J.; Woo, H.; Kim, Y.; Kim, G.; Seo, E.; Kang, H.; Kim, J.; Cho, D.; Kimura, A.; Kim, D.
    Optimal expression and characterization of a fusion enzyme having dextransucrase and dextranase activities (2010), Enzyme Microb. Technol., 47, 212-215.
No PubMed abstract available
Search for more literature for 2.4.1.5

Cloned(Commentary)

Cloned (Comment) Organism
gene dsxr, the recombinant chimeric dsxr encodes dextranase-dextransucrase, DXSR, is sequentially composed of the dextransucrase-encoding gene dsrBCB4 and the gene dex2, encoding the dextranase from Arthrobacter oxidans, UniProt ID A9UKG4, expression in Escherichia coli strain BL21(DE3)pLysS Leuconostoc mesenteroides

Protein Variants

Protein Variants Comment Organism
additional information construction and optimal expression of a fusion enzyme DSXR having dextransucrase and dextranase activities, for optimization of protein expression, response surface methodology is used Leuconostoc mesenteroides

Organism

Organism UniProt Comment Textmining
Leuconostoc mesenteroides D2CFL0 gene dsrBCB4
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
 120.1 U/ml, recombinant dextransucrase activity of recombinant DSXR chimeric enzyme, pH 5.8, 28°C Leuconostoc mesenteroides
1.2
-
 purified recombinant chimeric DSXR, dextransucrase activity, pH 5.8, 28°C Leuconostoc mesenteroides

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
28
-
 assay at Leuconostoc mesenteroides

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5.2
-
-
 Leuconostoc mesenteroides

pH Range

pH Minimum pH Maximum Comment Organism
5 6.4 highly reduced activity below pH 5.0 and above pH 6.4 Leuconostoc mesenteroides