Application | Comment | Organism |
---|---|---|
synthesis | the enzyme is useful for production of L-ascorbic acid 2-glucoside for use as an antioxidant in industrial applications | Leuconostoc lactis |
Cloned (Comment) | Organism |
---|---|
DNA and amino acid sequence determination and analysis, sequence comparisons, expression of His-tagged glucansucrase in Escherichia coli | Leuconostoc lactis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.8 | - |
sucrose | pH 5.0, 30°C, recombinant enzyme | Leuconostoc lactis |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
165000 | - |
x * 165000, recombinant enzyme, SDS-PAGE | Leuconostoc lactis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Leuconostoc lactis | C7DT60 | isolated from lactic acid bacteria in Kimchi, a traditional Korean fermented food | - |
Leuconostoc lactis EG001 | C7DT60 | isolated from lactic acid bacteria in Kimchi, a traditional Korean fermented food | - |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged glucansucrase 2.4fold from Escherichia coli by nickel affinity chromatography | Leuconostoc lactis |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
126 | - |
purified recombinant enzyme | Leuconostoc lactis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
sucrose + L-ascorbic acid | recombinant glucansucrase | Leuconostoc lactis | D-fructose + L-ascorbic acid 2-glucoside | the glycosylated product has the potential as an antioxidant in industrial applications | ? | |
sucrose + L-ascorbic acid | recombinant glucansucrase | Leuconostoc lactis EG001 | D-fructose + L-ascorbic acid 2-glucoside | the glycosylated product has the potential as an antioxidant in industrial applications | ? | |
sucrose + [(1,6)-alpha-D-glucosyl]n | - |
Leuconostoc lactis | D-fructose + [(1,6)-alpha-D-glucosyl]n+1 | - |
? | |
sucrose + [(1,6)-alpha-D-glucosyl]n | - |
Leuconostoc lactis EG001 | D-fructose + [(1,6)-alpha-D-glucosyl]n+1 | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 165000, recombinant enzyme, SDS-PAGE | Leuconostoc lactis |
Synonyms | Comment | Organism |
---|---|---|
glucansucrase | - |
Leuconostoc lactis |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
- |
Leuconostoc lactis |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
10 | 42 | activity range of the recombinant enzyme, profile, overview | Leuconostoc lactis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5 | - |
- |
Leuconostoc lactis |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
3.5 | 6.8 | activity range of the recombinant enzyme, profile, overview | Leuconostoc lactis |