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Literature summary for 2.4.1.5 extracted from

  • Kim, Y.M.; Yeon, M.J.; Choi, N.S.; Chang, Y.H.; Jung, M.Y.; Song, J.J.; Kim, J.S.
    Purification and characterization of a novel glucansucrase from Leuconostoc lactis EG001 (2010), Microbiol. Res., 165, 384-391.
    View publication on PubMed

Application

Application Comment Organism
synthesis the enzyme is useful for production of L-ascorbic acid 2-glucoside for use as an antioxidant in industrial applications Leuconostoc lactis

Cloned(Commentary)

Cloned (Comment) Organism
DNA and amino acid sequence determination and analysis, sequence comparisons, expression of His-tagged glucansucrase in Escherichia coli Leuconostoc lactis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.8
-
sucrose pH 5.0, 30°C, recombinant enzyme Leuconostoc lactis

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
165000
-
x * 165000, recombinant enzyme, SDS-PAGE Leuconostoc lactis

Organism

Organism UniProt Comment Textmining
Leuconostoc lactis C7DT60 isolated from lactic acid bacteria in Kimchi, a traditional Korean fermented food
-
Leuconostoc lactis EG001 C7DT60 isolated from lactic acid bacteria in Kimchi, a traditional Korean fermented food
-

Purification (Commentary)

Purification (Comment) Organism
recombinant His-tagged glucansucrase 2.4fold from Escherichia coli by nickel affinity chromatography Leuconostoc lactis

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
126
-
purified recombinant enzyme Leuconostoc lactis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
sucrose + L-ascorbic acid recombinant glucansucrase Leuconostoc lactis D-fructose + L-ascorbic acid 2-glucoside the glycosylated product has the potential as an antioxidant in industrial applications ?
sucrose + L-ascorbic acid recombinant glucansucrase Leuconostoc lactis EG001 D-fructose + L-ascorbic acid 2-glucoside the glycosylated product has the potential as an antioxidant in industrial applications ?
sucrose + [(1,6)-alpha-D-glucosyl]n
-
Leuconostoc lactis D-fructose + [(1,6)-alpha-D-glucosyl]n+1
-
?
sucrose + [(1,6)-alpha-D-glucosyl]n
-
Leuconostoc lactis EG001 D-fructose + [(1,6)-alpha-D-glucosyl]n+1
-
?

Subunits

Subunits Comment Organism
? x * 165000, recombinant enzyme, SDS-PAGE Leuconostoc lactis

Synonyms

Synonyms Comment Organism
glucansucrase
-
Leuconostoc lactis

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
-
Leuconostoc lactis

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
10 42 activity range of the recombinant enzyme, profile, overview Leuconostoc lactis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5
-
-
Leuconostoc lactis

pH Range

pH Minimum pH Maximum Comment Organism
3.5 6.8 activity range of the recombinant enzyme, profile, overview Leuconostoc lactis