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Literature summary for 2.4.1.5 extracted from

  • Yi, A.R.; Lee, S.R.; Jang, M.U.; Park, J.M.; Eom, H.J.; Han, N.S.; Kim, T.J.
    Cloning of dextransucrase gene from Leuconostoc citreum HJ-P4 and its high-level expression in E. coli by low temperature induction (2009), J. Microbiol. Biotechnol., 19, 829-835.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
gene LcDS, DNA and amino acid sequence determination and analysis, cloning in Escherichia coli strain MC1061, expression in Escherichia coli strain BL21 Leuconostoc citreum

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ activates 2.1fold at 0.1 mM, no activation at 5 mM Leuconostoc citreum

Organism

Organism UniProt Comment Textmining
Leuconostoc citreum A9ZRX3 gene LcDS
-
Leuconostoc citreum HJ-P4 A9ZRX3 gene LcDS
-

Source Tissue

Source Tissue Comment Organism Textmining
additional information induced recombinant enzyme production is 330fold higher at 15°C compared to 37°C Leuconostoc citreum
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
686
-
purified recombinant enzyme, in presence of 0.1 mM Ca2+ Leuconostoc citreum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information the recombinant enzyme successfully produces a series of isomaltooligosaccharides from sucrose and maltose, on the basis of its transglycosylation activity Leuconostoc citreum ?
-
?
additional information the recombinant enzyme successfully produces a series of isomaltooligosaccharides from sucrose and maltose, on the basis of its transglycosylation activity Leuconostoc citreum HJ-P4 ?
-
?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
35
-
-
Leuconostoc citreum

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
15 37 stable, rapid decrease in activity above 37°C Leuconostoc citreum

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5.5
-
-
Leuconostoc citreum

pH Range

pH Minimum pH Maximum Comment Organism
3.5 8 activity range, dependent on the buffer system Leuconostoc citreum

pH Stability

pH Stability pH Stability Maximum Comment Organism
4 8.5 LcDS is very stable within that pH range Leuconostoc citreum