Cloned (Comment) | Organism |
---|---|
gene LcDS, DNA and amino acid sequence determination and analysis, cloning in Escherichia coli strain MC1061, expression in Escherichia coli strain BL21 | Leuconostoc citreum |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | activates 2.1fold at 0.1 mM, no activation at 5 mM | Leuconostoc citreum |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Leuconostoc citreum | A9ZRX3 | gene LcDS | - |
Leuconostoc citreum HJ-P4 | A9ZRX3 | gene LcDS | - |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
additional information | induced recombinant enzyme production is 330fold higher at 15°C compared to 37°C | Leuconostoc citreum | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
686 | - |
purified recombinant enzyme, in presence of 0.1 mM Ca2+ | Leuconostoc citreum |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | the recombinant enzyme successfully produces a series of isomaltooligosaccharides from sucrose and maltose, on the basis of its transglycosylation activity | Leuconostoc citreum | ? | - |
? | |
additional information | the recombinant enzyme successfully produces a series of isomaltooligosaccharides from sucrose and maltose, on the basis of its transglycosylation activity | Leuconostoc citreum HJ-P4 | ? | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
35 | - |
- |
Leuconostoc citreum |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
15 | 37 | stable, rapid decrease in activity above 37°C | Leuconostoc citreum |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5.5 | - |
- |
Leuconostoc citreum |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
3.5 | 8 | activity range, dependent on the buffer system | Leuconostoc citreum |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
4 | 8.5 | LcDS is very stable within that pH range | Leuconostoc citreum |