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Literature summary for 2.4.1.5 extracted from

  • Yalin, Y.; Jin, L.; Jianhua, W.; Da, T.; Zigang, T.
    Expression and characterization of dextransucrase gene dsrX from Leuconostoc mesenteroides in Escherichia coli (2008), J. Biotechnol., 133, 505-512.
    View publication on PubMed
Search for more literature for 2.4.1.5

Cloned(Commentary)

Cloned (Comment) Organism
gene dsrX, DNA and amino acid sequence determination and analysis, cloning in Escherichia coli strain DH5alpha, expressed as a N-terminal His6-tagged protein Escherichia coli strain BL21(DE3), optimization of the recombinant expression system Leuconostoc mesenteroides

Inhibitors

Inhibitors Comment Organism Structure
Al3+ strongly inhibits the native and the recombinant enzyme Leuconostoc mesenteroides
Cu2+ strongly inhibits the native and the recombinant enzyme Leuconostoc mesenteroides
EDTA inhibits the native and the recombinant enzyme Leuconostoc mesenteroides
Fe2+ slightly inhibits the native, but not the recombinant enzyme Leuconostoc mesenteroides
Mg2+ slightly inhibits the native, but not the recombinant enzyme Leuconostoc mesenteroides
Na+ slightly inhibits the native, but not the recombinant enzyme Leuconostoc mesenteroides
Ni2+ slightly inhibits the native and the recombinant enzyme Leuconostoc mesenteroides
SDS inhibits the native and the recombinant enzyme Leuconostoc mesenteroides
Zn2+ slightly inhibits the native and the recombinant enzyme Leuconostoc mesenteroides

Metals/Ions

Metals/Ions Comment Organism Structure
Co2+ slightly activates the native and the recombinant enzyme Leuconostoc mesenteroides
Mn2+ activates the native and the recombinant enzyme Leuconostoc mesenteroides
additional information the enzyme is not or poorly affected by Ca2+, K+, and pepstatin Leuconostoc mesenteroides

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
167570
-
 x * 167570, recombinant His6-tagged DsrX, sequence calculation Leuconostoc mesenteroides

Organism

Organism UniProt Comment Textmining
Leuconostoc mesenteroides
-
 gene dsrX
-
Leuconostoc mesenteroides CGMCC 1.544
-
 gene dsrX
-

Purification (Commentary)

Purification (Comment) Organism
native enzyme partially by ammonium sulfate fractionation and dialysis, recombinant N-terminally His6-tagged DsrX from Escherichia coli strain BL21(DE3) by nickel affinity chromatography Leuconostoc mesenteroides

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
97.37
-
 recombinant enzyme in crude enzyme extract Leuconostoc mesenteroides

Subunits

Subunits Comment Organism
? x * 167570, recombinant His6-tagged DsrX, sequence calculation Leuconostoc mesenteroides

Synonyms

Synonyms Comment Organism
DsrX
-
 Leuconostoc mesenteroides

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
 recombinant enzyme Leuconostoc mesenteroides
40
-
 native enzyme Leuconostoc mesenteroides

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
4 60 the native DsrX shows higher stability than the recombinant DsrX at the temperature range from 4°C to 60°C for 2 h, native enzyme shows 84% remaining activity at 40°C after 2 h, while recombinant enzyme shows only 3.1% Leuconostoc mesenteroides

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5.4
-
 native and recombinant enzyme Leuconostoc mesenteroides

pH Range

pH Minimum pH Maximum Comment Organism
3 8
-
 Leuconostoc mesenteroides

pH Stability

pH Stability pH Stability Maximum Comment Organism
4 5 purified recombinant enzyme, quite stable Leuconostoc mesenteroides