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Literature summary for 2.4.1.5 extracted from

  • Suwannarangsee, S.; Moulis, C.; Potocki-Veronese, G.; Monsan, P.; Remaud-Simeon, M.; Chulalaksananukul, W.
    Search for a dextransucrase minimal motif involved in dextran binding (2007), FEBS Lett., 581, 4675-4680.
    View publication on PubMed

Application

Application Comment Organism
biotechnology potential application of the C-terminal enzyme domain GBD-7 as an affinity tag onto cheap resins like for rapid purification of dextrans Leuconostoc mesenteroides

Cloned(Commentary)

Cloned (Comment) Organism
expression of thioredoxin-His-tagged wild-type and trunacted mutant C-terminal domain GBD-7 Leuconostoc mesenteroides

Protein Variants

Protein Variants Comment Organism
additional information construction of fourteen truncated forms of strain NRRL B512-F dextransucrase by N-, C- or N- plus C-terminal domain truncations, dextran binding properties of mutant enzymes, overview Leuconostoc mesenteroides

Organism

Organism UniProt Comment Textmining
Leuconostoc mesenteroides
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Leuconostoc mesenteroides NRRL B512-F
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Purification (Commentary)

Purification (Comment) Organism
recombinant thioredoxin-His-tagged wild-type and truncated mutant C-terminal domain GBD-7 about 6fold by nickel affinity chromatography Leuconostoc mesenteroides

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
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dextran binding capacity of recombinant wild-type and truncated mutant C-terminal domain GBD-7, overview Leuconostoc mesenteroides

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
sucrose + dextran glucose transfer reaction, determination of the dextransucrase minimal motif involved in dextran binding, strong interaction with dextran is localized between amino acids N1397 and A1527 of the C-terminal domain, GBD-7, and consists of six YG repeats, overview. The motif containing enzyme shows very high affinity for isomaltohexaose and longer dextrans and is involved in polymer formation, overview Leuconostoc mesenteroides ?
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?
sucrose + dextran glucose transfer reaction, determination of the dextransucrase minimal motif involved in dextran binding, strong interaction with dextran is localized between amino acids N1397 and A1527 of the C-terminal domain, GBD-7, and consists of six YG repeats, overview. The motif containing enzyme shows very high affinity for isomaltohexaose and longer dextrans and is involved in polymer formation, overview Leuconostoc mesenteroides NRRL B512-F ?
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?
sucrose + isomaltohexaose glucose transfer reaction, determination of the dextransucrase minimal motif involved in dextran binding, strong interaction with dextran is localized between amino acids N1397 and A1527 of the C-terminal domain, GBD-7, and consists of six YG repeats, overview. The motif containing enzyme shows very high affinity for isomaltohexaose and longer dextrans and is involved in polymer formation, overview Leuconostoc mesenteroides ?
-
?
sucrose + isomaltohexaose glucose transfer reaction, determination of the dextransucrase minimal motif involved in dextran binding, strong interaction with dextran is localized between amino acids N1397 and A1527 of the C-terminal domain, GBD-7, and consists of six YG repeats, overview. The motif containing enzyme shows very high affinity for isomaltohexaose and longer dextrans and is involved in polymer formation, overview Leuconostoc mesenteroides NRRL B512-F ?
-
?