Cloned (Comment) | Organism |
---|---|
gene DSRB742, expression of wild-type and DRN1-DRN4 mutant enzymes in Escherichia coli strain BL21(DE3), subcloning in Escherichia coli strain DH5alpha | Leuconostoc mesenteroides |
Protein Variants | Comment | Organism |
---|---|---|
F196S | site-directed mutagenesis, the mutant DRN1 shows a higher expression level in Escherichia coli and increased activity compared to DSRB742 | Leuconostoc mesenteroides |
K395T | site-directed mutagenesis, the mutant DRN3 shows a higher expression level in Escherichia coli and increased activity compared to DSRB742 | Leuconostoc mesenteroides |
P980T | site-directed mutagenesis, the mutant DRN4 shows a lower expression level in Escherichia coli compared to DSRB742 | Leuconostoc mesenteroides |
Y346N | site-directed mutagenesis, the mutant DRN2 shows a lower expression level in Escherichia coli compared to DSRB742 | Leuconostoc mesenteroides |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
18 | - |
sucrose | pH 5.2, 28°C, recombinant mutant DRN1 | Leuconostoc mesenteroides | |
48 | - |
sucrose | pH 5.2, 28°C, recombinant mutant DRN3 | Leuconostoc mesenteroides | |
79 | - |
sucrose | pH 5.2, 28°C, recombinant mutant DRN4 | Leuconostoc mesenteroides | |
83 | - |
sucrose | pH 5.2, 28°C, recombinant mutant DRN2 | Leuconostoc mesenteroides | |
88 | - |
sucrose | pH 5.2, 28°C, recombinant wild-type enzyme | Leuconostoc mesenteroides |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Leuconostoc mesenteroides | - |
gene DSRB742 | - |
Purification (Comment) | Organism |
---|---|
recombinant wild-type and DRN1-DRN4 mutant enzymes from Escherichia coli by nickel affinity chromatography | Leuconostoc mesenteroides |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.012 | - |
purified recombinant mutant DRN4 | Leuconostoc mesenteroides |
0.027 | - |
purified recombinant mutant DRN2 | Leuconostoc mesenteroides |
0.037 | - |
purified recombinant wild-type DSRB742 | Leuconostoc mesenteroides |
0.185 | - |
purified recombinant mutant DRN3 | Leuconostoc mesenteroides |
0.287 | - |
purified recombinant mutant DRN1 | Leuconostoc mesenteroides |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
sucrose + gentobiose | glucose transfer from donor sucrose to acceptors releasing D-fructose, acceptor specificity of wild-type and mutant enzymes, overview | Leuconostoc mesenteroides | ? | - |
? | |
sucrose + maltose | glucose transfer from donor sucrose to acceptors releasing D-fructose, acceptor specificity of wild-type and mutant enzymes, overview | Leuconostoc mesenteroides | ? | - |
? | |
sucrose + salicin | glucose transfer from donor sucrose to acceptors releasing D-fructose, acceptor specificity of wild-type and mutant enzymes, overview | Leuconostoc mesenteroides | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
DSRB742 | - |
Leuconostoc mesenteroides |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
28 | - |
assay at | Leuconostoc mesenteroides |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.4 | - |
sucrose | pH 5.2, 28°C, recombinant mutant DRN4 | Leuconostoc mesenteroides | |
0.6 | - |
sucrose | pH 5.2, 28°C, recombinant mutant DRN2 | Leuconostoc mesenteroides | |
1.8 | - |
sucrose | pH 5.2, 28°C, recombinant wild-type enzyme | Leuconostoc mesenteroides | |
2.4 | - |
sucrose | pH 5.2, 28°C, recombinant mutant DRN1 | Leuconostoc mesenteroides | |
2.8 | - |
sucrose | pH 5.2, 28°C, recombinant mutant DRN3 | Leuconostoc mesenteroides |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5.2 | - |
assay at | Leuconostoc mesenteroides |