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Literature summary for 2.4.1.5 extracted from

  • de Segura, A.G.; Alcalde, M.; Bernabe, M.; Ballesteros, A.; Plou, F.J.
    Synthesis of methyl alpha-D-glucooligosaccharides by entrapped dextransucrase from Leuconostoc mesenteroides B-1299 (2006), J. Biotechnol., 124, 439-445.
    View publication on PubMed

Application

Application Comment Organism
synthesis potential use of the enzyme in production of glucooligosaccharides containing alpha(1,2) bonds for the dermocosmetic industry Leuconostoc mesenteroides

Organism

Organism UniProt Comment Textmining
Leuconostoc mesenteroides
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Leuconostoc mesenteroides B-1299
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Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
sucrose + alpha-D-glucopyranoside three homologous series (S1–S3) of methyl alpha-D-glucooligosaccharides. Series S2 and S3 are characterized by the presence of alpha(1,2) linkages, in combination with alpha(1,6) bonds. Two parameters, sucrose to acceptor concentration ratio (S/A) and the total sugar concentration (TSC) determine the yield of methyl alpha-D-glucooligosaccharides. The maximum concentration achieved of the first acceptor product, methyl alpha-D-isomaltoside, is 65 mM using a S/A 1:4 and a TSC of 336 g/l. When increasing temperature, a shift of selectivity towards compounds containing alpha(1,2) bonds is observed. The formation of leucrose as a side process reaches values of 32 g/l at high sucrose concentrations Leuconostoc mesenteroides ?
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?
sucrose + alpha-D-glucopyranoside three homologous series (S1–S3) of methyl alpha-D-glucooligosaccharides. Series S2 and S3 are characterized by the presence of alpha(1,2) linkages, in combination with alpha(1,6) bonds. Two parameters, sucrose to acceptor concentration ratio (S/A) and the total sugar concentration (TSC) determine the yield of methyl alpha-D-glucooligosaccharides. The maximum concentration achieved of the first acceptor product, methyl alpha-D-isomaltoside, is 65 mM using a S/A 1:4 and a TSC of 336 g/l. When increasing temperature, a shift of selectivity towards compounds containing alpha(1,2) bonds is observed. The formation of leucrose as a side process reaches values of 32 g/l at high sucrose concentrations Leuconostoc mesenteroides B-1299 ?
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?