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Literature summary for 2.4.1.5 extracted from
- Purification and properties of dextransucrase from Streptococcus mutans (1974), J. Bacteriol., 118, 1-7.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 2-mercaptoethanol | 120 mM, 17% inhibition | Streptococcus mutans |  |
| Ag+ | 1 mM, 94% inhibition | Streptococcus mutans | |
| D-fructose | competitive | Streptococcus mutans | |
| dithiothreitol | 100 mM, 41% inhibition | Streptococcus mutans | |
| Hg2+ | 1 mM, 35% inhibition | Streptococcus mutans | |
| SDS | 3.5 M; complete inhibition | Streptococcus mutans | |
| Urea | 8 M, complete inhibition | Streptococcus mutans | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 3 | - |
sucrose | - |
Streptococcus mutans | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 94000 | - |
gel filtration | Streptococcus mutans |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Streptococcus mutans | - |
6715 | - |
| Streptococcus mutans 6715 | - |
6715 | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| 6715 | Streptococcus mutans |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| culture medium | - |
Streptococcus mutans | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
- |
Streptococcus mutans |
Storage Stability
| Storage Stability | Organism |
|---|---|
| 4°C or -70°C, stable during extended storage | Streptococcus mutans |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| sucrose + (1,6-alpha-D-glucosyl)n | - |
Streptococcus mutans | D-fructose + (1,6-alpha-D-glucosyl)n+1 | - |
? | |
| sucrose + (1,6-alpha-D-glucosyl)n | - |
Streptococcus mutans 6715 | D-fructose + (1,6-alpha-D-glucosyl)n+1 | - |
? | |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 34 | 42 | - |
Streptococcus mutans |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 45 | - |
20 min, substantial loss of activity above | Streptococcus mutans |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 5.5 | - |
- |
Streptococcus mutans |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 27 | - |
fructose | - |
Streptococcus mutans | |
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