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Literature summary for 2.4.1.5 extracted from

  • Kobayashi, M.; Mihara, K.; Matsuda, K.
    Dextransucrase from Leuconostoc mesenteroides NRRL B-512F: Characterization of the enzyme bound to Sephadex gel (1986), Agric. Biol. Chem., 50, 551-556.
No PubMed abstract available
Search for more literature for 2.4.1.5

Activating Compound

Activating Compound Comment Organism Structure
Dextrans stimulates reducing sugar production 1.3fold and dextran synthesis 9.6fold Leuconostoc mesenteroides
methyl-alpha-D-glucoside 50 mM, activates release of D-fructose, sucrase activity, inhibits synthesis of dextran, transferase activity Leuconostoc mesenteroides

General Stability

General Stability Organism
stability is significantly increased by 33% glycerol and 0.1% bovine serum albumin Leuconostoc mesenteroides

Inhibitors

Inhibitors Comment Organism Structure
EDTA
-
 Leuconostoc mesenteroides
methyl-alpha-D-glucoside 50 mM, activates release of D-fructose (sucrase activity), inhibits synthesis of dextran (transferase activity) Leuconostoc mesenteroides

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information
-
 Leuconostoc mesenteroides

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ activates Leuconostoc mesenteroides

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
65000
-
 x * 65000, enzyme II, SDS-PAGE Leuconostoc mesenteroides

Organism

Organism UniProt Comment Textmining
Leuconostoc mesenteroides
-
 NRRl B-512(F)
-
Leuconostoc mesenteroides NRRl B-512(F)
-
 NRRl B-512(F)
-

Purification (Commentary)

Purification (Comment) Organism
dextransucrase II Leuconostoc mesenteroides
NRRL B-512(F) Leuconostoc mesenteroides

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
 Leuconostoc mesenteroides

Storage Stability

Storage Stability Organism
in a deep-freezer, 33% glycerol, 0.1% bovine serum albumin, less than 20% loss of activity after several months Leuconostoc mesenteroides

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
sucrose + (1,6-alpha-D-glucosyl)n
-
 Leuconostoc mesenteroides D-fructose + (1,6-alpha-D-glucosyl)n+1
-
 ?
sucrose + (1,6-alpha-D-glucosyl)n
-
 Leuconostoc mesenteroides NRRl B-512(F) D-fructose + (1,6-alpha-D-glucosyl)n+1
-
 ?

Subunits

Subunits Comment Organism
? x * 65000, enzyme II, SDS-PAGE Leuconostoc mesenteroides

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
-
 Leuconostoc mesenteroides

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5.2
-
-
 Leuconostoc mesenteroides