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Literature summary for 2.4.1.5 extracted from
- Characterization of the multiple forms and main component of dextransucrase from Leuconostoc mesenteroides NRRL B-512F (1980), Biochim. Biophys. Acta, 614, 46-62.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| Dextrans | stimulation | Leuconostoc mesenteroides |  |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Cu2+ | - |
Leuconostoc mesenteroides | |
| EDTA | addition of Ca2+ or Co2+ restores activity; addition of dextran partially protects | Leuconostoc mesenteroides | |
| Fe2+ | - |
Leuconostoc mesenteroides | |
| Fe3+ | - |
Leuconostoc mesenteroides | |
| Mn2+ | - |
Leuconostoc mesenteroides | |
| phenylmercuric acetate | - |
Leuconostoc mesenteroides | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | - |
Leuconostoc mesenteroides |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| extracellular | - |
Leuconostoc mesenteroides | - |
- |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | 1 mM, slight activation | Leuconostoc mesenteroides | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 65000 | - |
enzyme I and II, disc gel electrophoresis | Leuconostoc mesenteroides |
| 100000 | - |
enzyme III, disc gel electrophoresis | Leuconostoc mesenteroides |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Leuconostoc mesenteroides | - |
NRRl B-512(F) | - |
| Leuconostoc mesenteroides NRRl B-512(F) | - |
NRRl B-512(F) | - |
Posttranslational Modification
| Posttranslational Modification | Comment | Organism |
|---|---|---|
| glycoprotein | 17% carbohydrate | Leuconostoc mesenteroides |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| multiple forms: I, II and III | Leuconostoc mesenteroides |
| NRRL B-512(F) | Leuconostoc mesenteroides |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| culture medium | - |
Leuconostoc mesenteroides | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 72.1 | - |
- |
Leuconostoc mesenteroides |
Storage Stability
| Storage Stability | Organism |
|---|---|
| 4°C, pH 6.0-9.0, 4 mg/ml dextran, 24 h stable, without dextran the stable range is narrowed to pH 7.0-9.0 | Leuconostoc mesenteroides |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | the enzyme possesses enhanced levels of sucrose hydrolyzing activity | Leuconostoc mesenteroides | ? | - |
? | |
| additional information | the enzyme possesses enhanced levels of sucrose hydrolyzing activity | Leuconostoc mesenteroides NRRl B-512(F) | ? | - |
? | |
| sucrose + (1,6-alpha-D-glucosyl)n | - |
Leuconostoc mesenteroides | D-fructose + (1,6-alpha-D-glucosyl)n+1 | - |
? | |
| sucrose + (1,6-alpha-D-glucosyl)n | - |
Leuconostoc mesenteroides NRRl B-512(F) | D-fructose + (1,6-alpha-D-glucosyl)n+1 | - |
? | |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
- |
Leuconostoc mesenteroides |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 20 | 40 | 20°C: about 60% of maximal activity, 40°C: about 50% of maximal activity | Leuconostoc mesenteroides |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 40 | - |
10 min, 80% loss of activity without dextran, 30% loss of activity with addition of dextran | Leuconostoc mesenteroides |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6 | - |
- |
Leuconostoc mesenteroides |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 5 | 7 | about 40% of maximal activity at pH 5.0 and pH 7.0 | Leuconostoc mesenteroides |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 6 | 9 | 4°C, stable at 4°C for 24 h, 4 mg/ml dextrans | Leuconostoc mesenteroides |
| 7 | 9 | 4°C, stable for 24 h, without dextran in solution | Leuconostoc mesenteroides |
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