BRENDA - Enzyme Database
show all sequences of 2.4.1.47

Purification and characterization of UDP-gal:ceramide galactosyltransferase and reconstitution of its activity by incorporation into liposomes

Berardi, A.; Fabrizi, G.M.; Manetti, R.; Costantino-Ceccarini, E.; NATO ASI Ser. A, Life Sci. 150, 681-687 (1988)
No PubMed abstract available

Data extracted from this reference:

Activating Compound
Activating Compound
Commentary
Organism
Structure
phosphatidylcholine
optimum phospholipid requirement of phosphatidylethanolamine: phosphatidylcholine of 5:1 with 2.5fold stimulation, Na cholate prevents stimulation
Rattus norvegicus
phosphatidylethanolamine
optimum phospholipid requirement of phosphatidylethanolamine: phosphatidylcholine of 5:1 with 2.5fold stimulation, Na cholate prevents stimulation
Rattus norvegicus
Phospholipids
optimum phospholipid requirement of phosphatidylethanolamine: phosphatidylcholine of 5:1 with 2.5fold stimulation, Na cholate prevents stimulation
Rattus norvegicus
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
53000
-
x * 53000, SDS-PAGE
Rattus norvegicus
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Rattus norvegicus
-
15-16 days old
-
Purification (Commentary)
Commentary
Organism
-
Rattus norvegicus
Source Tissue
Source Tissue
Commentary
Organism
Textmining
brain
-
Rattus norvegicus
-
Subunits
Subunits
Commentary
Organism
?
x * 53000, SDS-PAGE
Rattus norvegicus
Activating Compound (protein specific)
Activating Compound
Commentary
Organism
Structure
phosphatidylcholine
optimum phospholipid requirement of phosphatidylethanolamine: phosphatidylcholine of 5:1 with 2.5fold stimulation, Na cholate prevents stimulation
Rattus norvegicus
phosphatidylethanolamine
optimum phospholipid requirement of phosphatidylethanolamine: phosphatidylcholine of 5:1 with 2.5fold stimulation, Na cholate prevents stimulation
Rattus norvegicus
Phospholipids
optimum phospholipid requirement of phosphatidylethanolamine: phosphatidylcholine of 5:1 with 2.5fold stimulation, Na cholate prevents stimulation
Rattus norvegicus
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
53000
-
x * 53000, SDS-PAGE
Rattus norvegicus
Purification (Commentary) (protein specific)
Commentary
Organism
-
Rattus norvegicus
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
brain
-
Rattus norvegicus
-
Subunits (protein specific)
Subunits
Commentary
Organism
?
x * 53000, SDS-PAGE
Rattus norvegicus
Other publictions for EC 2.4.1.47
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
703918
Niimura
Modification of sphingoglycoli ...
Canis lupus familiaris, Chlorocebus sabaeus
Glycobiology
20
711-717
2010
1
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1
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4
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2
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2
-
-
662680
Fewou
Reversal of non-hydroxy:alpha- ...
Rattus norvegicus
J. Neurochem.
94
469-481
2005
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-
-
-
1
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1
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1
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662811
Sprong
Association of the Golgi UDP-g ...
Rattus norvegicus
Mol. Biol. Cell
14
3482-3493
2003
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489224
Tencomnao
Characterization of the human ...
Homo sapiens
Biochim. Biophys. Acta
1517
416-423
2001
-
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1
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489221
Kapitonov
Cloning, characterization, and ...
Gallus gallus, Homo sapiens
Biochem. Biophys. Res. Commun.
232
449-453
1997
-
1
2
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1
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1
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5
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1
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2
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2
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489220
Bosio
Molecular cloning and characte ...
Homo sapiens, Mus musculus, Rattus norvegicus
Genomics
35
223-226
1996
-
-
3
-
-
-
-
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4
-
1
3
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4
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2
1
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2
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6
1
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3
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1
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2
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6
1
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489222
Bosio
The human gene CGT encoding th ...
Homo sapiens
Genomics
34
69-75
1996
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1
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2
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1
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2
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489225
Schulte
UDP galactose:ceramide galacto ...
Rattus norvegicus
Eur. J. Biochem.
233
947-953
1995
-
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1
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2
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2
2
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1
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1
1
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3
1
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1
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489223
Padmini
UDP galactose:ceramide galacto ...
Rattus norvegicus
J. Neurosci. Res.
23
310-315
1989
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1
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1
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489219
Berardi
-
Purification and characterizat ...
Rattus norvegicus
NATO ASI Ser. A, Life Sci.
150
681-687
1988
3
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1
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489216
Neskovic
UDPgalactose: ceramide galacto ...
Rattus norvegicus
Methods Enzymol.
71
521-527
1981
3
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2
7
3
1
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1
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2
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1
1
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2
1
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5
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1
5
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489217
Constantino-Ceccarini
A novel assay method for the b ...
Rattus norvegicus
Methods Enzymol.
72
384-391
1981
-
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1
1
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2
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489218
Neskovic
Brain UDPgalactose: ceramide g ...
Rattus norvegicus
Biochim. Biophys. Acta
429
342-351
1976
-
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2
2
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2
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1
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1
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489215
Basu
Enzymatic synthesis of galacto ...
Cavia porcellus, Gallus gallus, Rattus norvegicus, Sus scrofa
J. Biol. Chem.
246
4272-4279
1971
-
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1
2
2
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6
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2
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4
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8
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1
8
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2
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1
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489214
Morell
Synthesis of cerebroside by br ...
Mus musculus
Biochemistry
8
506-512
1969
-
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1
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6
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489240
Fujino
Enzymic synthesis of cerebrosi ...
Rattus norvegicus
Biochem. J.
113
573-575
1969
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2
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