Activating Compound | Comment | Organism | Structure |
---|---|---|---|
phosphatidic acid | allosterically activates MGD1, half-maximal activation at about 0.2 mol%. With 0.15 mol% of phosphatidic acid, the enzyme velocity versus substrate curve is sigmoid, whereas with 1.5 mol% of phosphatidic acid, the curve is hyperbolic. MGDG synthase activity of leaf homogenates is dependent on the constant presence of phosphatidic acid, decrease of phosphatidic acid production leads to a decrease of the MGDG synthase activity. Molecular discrimination of phosphatidic acid and phosphatidylglycerol binding sites, overview | Arabidopsis thaliana |
Cloned (Comment) | Organism |
---|---|
expression of wild-type and mutant C-terminally His6-tagged MGD1 in Escherichia coli | Arabidopsis thaliana |
Protein Variants | Comment | Organism |
---|---|---|
D150N | the mutant shows enhanced activation by phosphatidylglycerol binding, but unaltered activation by phosphatidic acid compared to the wild-type enzyme | Arabidopsis thaliana |
P189A | the mutant shows wild-type catalytic capability, but modified phosphatidylglycerol binding capability compared to the wild-type enzyme | Arabidopsis thaliana |
R260A | the mutant shows enhanced activation by phosphatidylglycerol binding, but unaltered activation by phosphatidic acid compared to the wild-type enzyme | Arabidopsis thaliana |
W287A | the mutant shows wild-type catalytic capability, but modified phosphatidylglycerol binding capability compared to the wild-type enzyme | Arabidopsis thaliana |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
chloroplast envelope | - |
Arabidopsis thaliana | 9941 | - |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Arabidopsis thaliana | regulation of MGDGsynthesis by phosphatidic acid, which is a general precursor in the synthesis of all glycerolipids and is also a signaling molecule in plants | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Arabidopsis thaliana | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant wild-type and mutant C-terminally His6-tagged MGD1 from Escherichia coli by nickel affinity chromatography and gel filtration | Arabidopsis thaliana |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
rosette leaf | - |
Arabidopsis thaliana | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | regulation of MGDGsynthesis by phosphatidic acid, which is a general precursor in the synthesis of all glycerolipids and is also a signaling molecule in plants | Arabidopsis thaliana | ? | - |
? | |
additional information | binding site for UDP-Gal lays in the cleft separating the two Rossmann folds involving residues of a conserved UDP-sugar binding pocket in the C-domain | Arabidopsis thaliana | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
More | the structure model for an MGD monomer comprises 2 Rossman domains, C- and N-domains | Arabidopsis thaliana |
Synonyms | Comment | Organism |
---|---|---|
MGD1 | - |
Arabidopsis thaliana |
MGDG synthase | - |
Arabidopsis thaliana |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.8 | - |
assay at | Arabidopsis thaliana |
General Information | Comment | Organism |
---|---|---|
physiological function | MGD1 is the main MGDG synthase expressed in leaves and is essential for chloroplast development, and enrichment of chloroplast membranes with monogalactosyldiacylglycerols | Arabidopsis thaliana |