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BRENDA support

Literature summary for 2.4.1.43 extracted from

  • Guillaumie, F.; Sterling, J.D.; Jensen, K.J.; Thomas, O.R.T.; Mohnen, D.
    Solid-supported enzymatic synthesis of pectic oligogalacturonides and their analysis by MALDI-TOF mass spectrometry (2003), Carbohydr. Res., 338, 1951-1960.
    View publication on PubMed

Application

Application Comment Organism
analysis development of enzyme-bound solid-phase support containing long-pectic oligogalacturonides anchored to the resin material via disulfide-containing cleavable linker, enzyme is reversibly bound, for performance of solid-phase biosynthetic reaction to investigate the pectin oligomer biosynthesis, product identification by MALDI-TOF mass spectrometry, overview Arabidopsis thaliana

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
UDP-D-galacturonate + (1,4-alpha-D-galacturonosyl)n Arabidopsis thaliana involved in biosynthesis of pectin oligomers, overview UDP + (1,4-alpha-D-galacturonosyl)n+1
-
?

Organism

Organism UniProt Comment Textmining
Arabidopsis thaliana
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cv. Columbia
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Purification (Commentary)

Purification (Comment) Organism
partially from dark-grown cells Arabidopsis thaliana

Source Tissue

Source Tissue Comment Organism Textmining
cell suspension culture from cells derived from leaf calli Arabidopsis thaliana
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.00095
-
partially purified enzyme Arabidopsis thaliana

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
UDP-D-galacturonate + (1,4-alpha-D-galacturonosyl)n
-
Arabidopsis thaliana UDP + (1,4-alpha-D-galacturonosyl)n+1
-
?
UDP-D-galacturonate + (1,4-alpha-D-galacturonosyl)n involved in biosynthesis of pectin oligomers, overview Arabidopsis thaliana UDP + (1,4-alpha-D-galacturonosyl)n+1
-
?

Synonyms

Synonyms Comment Organism
alpha-1,4-galacturonosyltransferase
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Arabidopsis thaliana
GalAT
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Arabidopsis thaliana

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
assay at Arabidopsis thaliana

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.8
-
assay at Arabidopsis thaliana