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Literature summary for 2.4.1.43 extracted from
- In vitro biosynthesis of homogalacturonan by a membrane-bound galacturonosyltransferase from epicotyls of azuki bean (2001), Biosci. Biotechnol. Biochem., 65, 1519-1527.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| Triton X-100 | 0.5% acceptor acid-soluble polygalacturonic acid, 170% increase in activity | Vigna angularis |  |
| Triton X-102 | 0.5%, acceptor acid-soluble polygalacturonic acid, 190% increase in activity | Vigna angularis |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| ADP | 10 mM, 60% inhibition | Vigna angularis | |
| ATP | 10 mM, 83% inhibition | Vigna angularis | |
| dithiothreitol | 10 mM, 61% inhibition | Vigna angularis | |
| EDTA | 10 mM, 82% inhibition | Vigna angularis | |
| EGTA | 10 mM, 96% inhibition | Vigna angularis | |
| GTP | 10 mM, 80% inhibition | Vigna angularis | |
| NaF | 10 mM, 56% inhibition | Vigna angularis | |
| S-adenosyl-L-methionine | 2 mM, 10% inhibition | Vigna angularis | |
| UTP | 10 mM, 86% inhibition | Vigna angularis | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | Km for acid-soluble polygalacturonic acid of 10000 Da: 0.02 mg/ml | Vigna angularis | |
| 0.14 | - |
UDP-D-galacturonic acid | - |
Vigna angularis | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| membrane | - |
Vigna angularis | 16020 | - |
| microsome | - |
Vigna angularis | - |
- |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| MnCl2 | 5 mM, 40fold stimulation | Vigna angularis | |
| Zn2+ | 5 mM, 8fold stimulation | Vigna angularis | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Vigna angularis | - |
azuki bean | - |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| seedling | - |
Vigna angularis | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.0013 | 0.002 | activity in microsomes | Vigna angularis |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| UDP-D-galacturonic acid + (1,4-alpha-D-galacturonosyl)n | acid-soluble polygalacturonic acid of 10000 Da is a better acceptor than acid-insoluble polygalacturonic acid of 70000 Da | Vigna angularis | UDP + (1,4-alpha-D-galacturonosyl)n+1 | - |
? | |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 25 | 35 | in the presence of 5 mM Mn2+ and 0.5% Triton X-100 | Vigna angularis |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
90 min, 505 loss of activity | Vigna angularis |
| 45 | - |
90 min, complete loss of activity | Vigna angularis |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6.8 | 7.8 | in the presence of 5 mM Mn2+ and 0.5% Triton X-100 | Vigna angularis |
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Release 2023.1 (January 2023)
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