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Literature summary for 2.4.1.41 extracted from

  • Herr, P.; Korniychuk, G.; Yamamoto, Y.; Grubisic, K.; Oelgeschlaeger, M.
    Regulation of TGF-beta signalling by N-acetylgalactosaminyltransferase-like 1 (2008), Development, 135, 1813-1822.
    View publication on PubMed

Application

Application Comment Organism
molecular biology overexpression in eukaryotic cell culture leads to inhibition of Activin/Nodal pathway activity in vivo by interference with binding of ActR-IIB to type I TGFbeta receptor proteins that can mediate BMP as well as Nodal signalling Xenopus laevis

Cloned(Commentary)

Cloned (Comment) Organism
Flag-tagged xGalnt-6 in pCS2+ for eukaryotic expression Xenopus laevis
ORF of xGalntl-1, a C-terminal HA-tagged and a Flag-tagged version in pCS2+ for eukaryotic expression Xenopus laevis

Protein Variants

Protein Variants Comment Organism
AA93-563 abbreviated as sGalntl-1, N-terminal transmembrane domain replaced by the Chordin N-terminal leader peptide followed by Flag-tag, no enhanced secretion compared to wild-type, altered activity in Xenopus embryo, no interference with binding of ActRII-B to type I TGFbeta receptor proteins Xenopus laevis

Inhibitors

Inhibitors Comment Organism Structure
benzyl-N-acetyl-alpha-D-galactosaminide benzyl-GalNAc, 2 mM over night in Hek293T cell culture, abolishes activity of transiently transfected xGalntl-1 in Hek293T cells like inhibition of Smad2 phosphorylation by ActR-IIB and induction of a migration shift of ActR-IIB and Alk-3 Xenopus laevis

Localization

Localization Comment Organism GeneOntology No. Textmining
extracellular based on detection of microinjected HA-tagged protein in the supernatant of dissociated animal ectodermal explants Xenopus laevis
-
-
Golgi apparatus transient transfection of Flag-tagged version in COS-7 cells Xenopus laevis 5794
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
additional information
-
in Western blot, slightly lower molecular weight of HA-tagged protein in the supernatant than in the cell pellet of dissociated animal ectodermal explants Xenopus laevis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
UDP-N-acetyl-D-galactosamine + polypeptide Xenopus laevis mucin-type O-linked glycosylation of serine or threonine residues UDP + N-acetyl-D-galactosaminyl-polypeptide
-
?

Organism

Organism UniProt Comment Textmining
Xenopus laevis
-
isozymes xGalnt1, -4, -6, -7
-

Posttranslational Modification

Posttranslational Modification Comment Organism
proteolytic modification assumed due to detection in supernatants of dissociated ectodermal cells at slightly lower molecular weight Xenopus laevis

Source Tissue

Source Tissue Comment Organism Textmining
embryo expression increases at later embryonal developmental stages Xenopus laevis
-
embryo RT-PCR, xGalnt1, -6, -7 expression throughout early and late embryonal development Xenopus laevis
-
gastrula RT-PCR, expression is restricted to dorsal marginal zones at embryonal stage 20 Xenopus laevis
-
gastrula RT-PCR, xGalnt1, -6, -7 expression in both, dorsal and ventral marginal zones at embryonal stage 20 Xenopus laevis
-
neurula RT-PCR, expression at embryonal stage 13 in anterior mesoderm and neural crest Xenopus laevis
-
tailbud stage RT-PCR, expression at embryonal stage 27 excluded from the central area of the mediolateral spinal cord containing proliferating neuronal precursors Xenopus laevis
-
tailbud stage RT-PCR, expression at embryonal stage 27 in forebrain Xenopus laevis
-
tailbud stage RT-PCR, expression at embryonal stage 27 in neural crest cells that migrate into heart regions, branchial arches and heart anlage Xenopus laevis
-
tailbud stage RT-PCR, expression at embryonal stage 27 in notochord (highest in posterior regions) Xenopus laevis
-
tailbud stage RT-PCR, expression at embryonal stage 27 in the mantle (differentiating) territory of the mediolateral spinal cord Xenopus laevis
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
UDP-GalNAc + polypeptide
-
Xenopus laevis GalNAc-alpha-polypeptide + UDP glycosylation at Thr or Ser ?
UDP-N-acetyl-D-galactosamine + polypeptide mucin-type O-linked glycosylation of serine or threonine residues Xenopus laevis UDP + N-acetyl-D-galactosaminyl-polypeptide
-
?

Synonyms

Synonyms Comment Organism
N-acetylgalactosaminyltransferase-like 1
-
Xenopus laevis
xGaltnl-1
-
Xenopus laevis