Application | Comment | Organism |
---|---|---|
molecular biology | overexpression in eukaryotic cell culture leads to inhibition of Activin/Nodal pathway activity in vivo by interference with binding of ActR-IIB to type I TGFbeta receptor proteins that can mediate BMP as well as Nodal signalling | Xenopus laevis |
Cloned (Comment) | Organism |
---|---|
Flag-tagged xGalnt-6 in pCS2+ for eukaryotic expression | Xenopus laevis |
ORF of xGalntl-1, a C-terminal HA-tagged and a Flag-tagged version in pCS2+ for eukaryotic expression | Xenopus laevis |
Protein Variants | Comment | Organism |
---|---|---|
AA93-563 | abbreviated as sGalntl-1, N-terminal transmembrane domain replaced by the Chordin N-terminal leader peptide followed by Flag-tag, no enhanced secretion compared to wild-type, altered activity in Xenopus embryo, no interference with binding of ActRII-B to type I TGFbeta receptor proteins | Xenopus laevis |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
benzyl-N-acetyl-alpha-D-galactosaminide | benzyl-GalNAc, 2 mM over night in Hek293T cell culture, abolishes activity of transiently transfected xGalntl-1 in Hek293T cells like inhibition of Smad2 phosphorylation by ActR-IIB and induction of a migration shift of ActR-IIB and Alk-3 | Xenopus laevis |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
extracellular | based on detection of microinjected HA-tagged protein in the supernatant of dissociated animal ectodermal explants | Xenopus laevis | - |
- |
Golgi apparatus | transient transfection of Flag-tagged version in COS-7 cells | Xenopus laevis | 5794 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
additional information | - |
in Western blot, slightly lower molecular weight of HA-tagged protein in the supernatant than in the cell pellet of dissociated animal ectodermal explants | Xenopus laevis |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
UDP-N-acetyl-D-galactosamine + polypeptide | Xenopus laevis | mucin-type O-linked glycosylation of serine or threonine residues | UDP + N-acetyl-D-galactosaminyl-polypeptide | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Xenopus laevis | - |
isozymes xGalnt1, -4, -6, -7 | - |
Posttranslational Modification | Comment | Organism |
---|---|---|
proteolytic modification | assumed due to detection in supernatants of dissociated ectodermal cells at slightly lower molecular weight | Xenopus laevis |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
embryo | expression increases at later embryonal developmental stages | Xenopus laevis | - |
embryo | RT-PCR, xGalnt1, -6, -7 expression throughout early and late embryonal development | Xenopus laevis | - |
gastrula | RT-PCR, expression is restricted to dorsal marginal zones at embryonal stage 20 | Xenopus laevis | - |
gastrula | RT-PCR, xGalnt1, -6, -7 expression in both, dorsal and ventral marginal zones at embryonal stage 20 | Xenopus laevis | - |
neurula | RT-PCR, expression at embryonal stage 13 in anterior mesoderm and neural crest | Xenopus laevis | - |
tailbud stage | RT-PCR, expression at embryonal stage 27 excluded from the central area of the mediolateral spinal cord containing proliferating neuronal precursors | Xenopus laevis | - |
tailbud stage | RT-PCR, expression at embryonal stage 27 in forebrain | Xenopus laevis | - |
tailbud stage | RT-PCR, expression at embryonal stage 27 in neural crest cells that migrate into heart regions, branchial arches and heart anlage | Xenopus laevis | - |
tailbud stage | RT-PCR, expression at embryonal stage 27 in notochord (highest in posterior regions) | Xenopus laevis | - |
tailbud stage | RT-PCR, expression at embryonal stage 27 in the mantle (differentiating) territory of the mediolateral spinal cord | Xenopus laevis | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
UDP-GalNAc + polypeptide | - |
Xenopus laevis | GalNAc-alpha-polypeptide + UDP | glycosylation at Thr or Ser | ? | |
UDP-N-acetyl-D-galactosamine + polypeptide | mucin-type O-linked glycosylation of serine or threonine residues | Xenopus laevis | UDP + N-acetyl-D-galactosaminyl-polypeptide | - |
? |
Synonyms | Comment | Organism |
---|---|---|
N-acetylgalactosaminyltransferase-like 1 | - |
Xenopus laevis |
xGaltnl-1 | - |
Xenopus laevis |