Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Bos taurus |
Protein Variants | Comment | Organism |
---|---|---|
D209A | inactive mutant enzyme | Bos taurus |
F124A | inactive mutant enzyme | Bos taurus |
L189A | inactive mutant enzyme | Bos taurus |
N126A | the turnover number for UDP-N-acetyl-D-galactosamine and STPSTPSTPSTPSTP is 1.4fold lower than the wild-type value, the KM-value for UDP-N-acetyl-D-galactosamine is 5.5fold higher than the wild-type value, the KM-value for STPSTPSTPSTPSTP is 1.6fold higher than the wild-type value | Bos taurus |
R193A | inactive mutant enzyme | Bos taurus |
W129A | the turnover number for UDP-N-acetyl-D-galactosamine and STPSTPSTPSTPSTP is 4.3fold lower than the wild-type value, the KM-value for UDP-N-acetyl-D-galactosamine is 3.5fold higher than the wild-type value, the KM-value for STPSTPSTPSTPSTP is 2.6fold higher than the wild-type value | Bos taurus |
W129F | the turnover number for UDP-N-acetyl-D-galactosamine and STPSTPSTPSTPSTP is 1.4fold lower than the wild-type value, the KM-value for UDP-N-acetyl-D-galactosamine is 1.8fold higher than the wild-type value, the KM-value for STPSTPSTPSTPSTP is 1.2fold lower than the wild-type value | Bos taurus |
W129R | the turnover number for UDP-N-acetyl-D-galactosamine and STPSTPSTPSTPSTP is 1.2fold lower than the wild-type value, the KM-value for UDP-N-acetyl-D-galactosamine is 2.1fold higher than the wild-type value, the KM-value for STPSTPSTPSTPSTP is 1.2fold lower than the wild-type value | Bos taurus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.008 | - |
UDP-N-acetyl-D-galactosamine | pH 6.5, 37°C, wild-type enzyme | Bos taurus | |
0.014 | - |
UDP-N-acetyl-D-galactosamine | pH 6.5, 37°C, mutant enzyme W129F | Bos taurus | |
0.017 | - |
UDP-N-acetyl-D-galactosamine | pH 6.5, 37°C, mutant enzyme W129R | Bos taurus | |
0.029 | - |
UDP-N-acetyl-D-galactosamine | pH 6.5, 37°C, mutant enzyme W129A | Bos taurus | |
0.044 | - |
UDP-N-acetyl-D-galactosamine | pH 6.5, 37°C, mutant enzyme N126A | Bos taurus | |
0.2 | - |
STPSTPSTPSTPSTP | pH 6.5, 37°C, mutant enzyme W129R | Bos taurus | |
0.205 | - |
STPSTPSTPSTPSTP | pH 6.5, 37°C, mutant enzyme W129F | Bos taurus | |
0.25 | - |
STPSTPSTPSTPSTP | pH 6.5, 37°C, wild-type enzyme | Bos taurus | |
0.41 | - |
STPSTPSTPSTPSTP | pH 6.5, 37°C, mutant enzyme N126A | Bos taurus | |
0.65 | - |
STPSTPSTPSTPSTP | pH 6.5, 37°C, mutant enzyme W129A | Bos taurus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mn2+ | in coordination both with the phosphates of UDP and the DXH motif of the enzyme | Bos taurus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bos taurus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant enzyme | Bos taurus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
UDP-N-acetyl-D-galactosamine + STPSTPSTPSTPSTP | - |
Bos taurus | UDP + ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
ppGaNTase | - |
Bos taurus |
UDP-GalNAc:polypeptide alphaN-acetylgalactosaminyltransferase | - |
Bos taurus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.8 | - |
UDP-N-acetyl-D-galactosamine | pH 6.5, 37°C, mutant enzyme W129A | Bos taurus | |
0.8 | - |
STPSTPSTPSTPSTP | pH 6.5, 37°C, mutant enzyme W129A | Bos taurus | |
2.43 | - |
UDP-N-acetyl-D-galactosamine | pH 6.5, 37°C, mutant enzyme W129F | Bos taurus | |
2.43 | - |
STPSTPSTPSTPSTP | pH 6.5, 37°C, mutant enzyme W129F | Bos taurus | |
2.53 | - |
UDP-N-acetyl-D-galactosamine | pH 6.5, 37°C, mutant enzyme N126A | Bos taurus | |
2.53 | - |
STPSTPSTPSTPSTP | pH 6.5, 37°C, mutant enzyme N126A | Bos taurus | |
2.79 | - |
UDP-N-acetyl-D-galactosamine | pH 6.5, 37°C, mutant enzyme W129R | Bos taurus | |
2.79 | - |
STPSTPSTPSTPSTP | pH 6.5, 37°C, mutant enzyme W129R | Bos taurus | |
3.47 | - |
UDP-N-acetyl-D-galactosamine | pH 6.5, 37°C, wild-type enzyme | Bos taurus | |
3.47 | - |
STPSTPSTPSTPSTP | pH 6.5, 37°C, wild-type enzyme | Bos taurus |