Cloned (Comment) | Organism |
---|---|
cloning of ppGalNAc-T1, -T2, -T3, -T4, -T6 and T9 and expression as secreted proteins fused with a FLAG peptide in insect cells | Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
UDP-N-acetyl-D-galactosamine + polypeptide | Homo sapiens | initiation of mucin-type O-glycosylation by a family of polypeptide GalNAc-transferases, of which each has a unique function | UDP + N-acetyl-D-galactosaminyl-polypeptide | - |
? | |
UDP-N-acetyl-D-galactosamine + polypeptide | Homo sapiens | ppGalNAc-T2: initiation of O-glycosylation in the IgA1 hinge region | UDP + N-acetyl-D-galactosaminyl-polypeptide | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant ppGalNAc-T1, -T2, -T3, -T4, -T6 and T9, expressed in insect cells | Homo sapiens |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
B-lymphocyte cell line | expression of ppGalNAc-T1, -T2, -T3, -T4, -T6 and -T9 in B-cells and IgA-bearing B cells | Homo sapiens | - |
myeloma cell line | expression of ppGalNAc-T1, -T2, -T3, -T4, -T6 and -T9 in NCI-H929 IgA myeloma cells | Homo sapiens | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | at least 11 human ppGalNAc-Ts | Homo sapiens | ? | - |
? | |
UDP-N-acetyl-D-galactosamine + GalNAc-glycosylated peptide | ppGalNAc-T4 and -T7 prefer peptides having GalNAc residues as acceptor substrates | Homo sapiens | UDP + N-acetyl-D-galactosaminyl-GalNAc-glucosylated peptide | - |
? | |
UDP-N-acetyl-D-galactosamine + immunoglobulin A1 | O-linked glycosylation of the human IgA1 hinge region by ppGalNAc-T2, synthetic IgA hinge peptide: ppGalNAc-T2 shows strongest activity and is able to transfer GalNAc to almost all possible glycosylation sites, other ppGalNAc-Ts show extremely weak activities | Homo sapiens | UDP + N-acetyl-D-galactosaminyl-immunoglobulin A1 | - |
? | |
UDP-N-acetyl-D-galactosamine + polypeptide | substrate specificities of recombinant ppGalNAc-T1, -T2, -T3, -T4, -T6 and T9 | Homo sapiens | UDP + N-acetyl-D-galactosaminyl-polypeptide | - |
? | |
UDP-N-acetyl-D-galactosamine + polypeptide | initiation of mucin-type O-glycosylation by a family of polypeptide GalNAc-transferases, of which each has a unique function | Homo sapiens | UDP + N-acetyl-D-galactosaminyl-polypeptide | - |
? | |
UDP-N-acetyl-D-galactosamine + polypeptide | ppGalNAc-T2: initiation of O-glycosylation in the IgA1 hinge region | Homo sapiens | UDP + N-acetyl-D-galactosaminyl-polypeptide | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Homo sapiens |