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Literature summary for 2.4.1.40 extracted from

  • Schwyzer, M.; Hill, R.L.
    Porcine A blood group-specific N-acetylgalactosaminyltransferase. I. Purification from porcine submaxillary glands (1977), J. Biol. Chem., 252, 2338-2345.
    View publication on PubMed

General Stability

General Stability Organism
irreversible inactivation in absence of Triton X-100 Sus scrofa
loss of activity after freezing in buffer of pH 7.5 Sus scrofa

Inhibitors

Inhibitors Comment Organism Structure
UMP
-
Sus scrofa

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
46000
-
2 * 46000, sedimentation equilibrium of reduced and carboxymethylated enzyme Sus scrofa
100000
-
zonal centrifugation, gel filtration Sus scrofa

Organism

Organism UniProt Comment Textmining
Sus scrofa
-
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein
-
Sus scrofa

Purification (Commentary)

Purification (Comment) Organism
-
Sus scrofa

Source Tissue

Source Tissue Comment Organism Textmining
submaxillary gland
-
Sus scrofa
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
30
-
-
Sus scrofa

Storage Stability

Storage Stability Organism
-70°C, 0.025 M MES buffer, pH 6.0, 1 mM EDTA, 0.03% Triton X-100, after removal of most of the buffer by dialysis, stable for 1 year Sus scrofa
4°C, pH 7.5, half-life is about 3 months Sus scrofa

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
UDP-N-acetyl-D-galactosamine + asialo-porcine submaxillary mucin of A-negative blood-type
-
Sus scrofa UDP + N-acetyl-alpha-D-galactosaminyl-1,3-[asialo-porcine submaxillary mucin of A-negative blood-type]
-
?
UDP-N-acetyl-D-galactosamine + glycoprotein alpha-L-fucosyl-1,2-D-galactose
-
Sus scrofa UDP + glycoprotein N-acetyl-alpha-D-galactosaminyl-1,3-[alpha-L-fucosyl-1,2]-D-galactose
-
?

Subunits

Subunits Comment Organism
dimer 2 * 46000, sedimentation equilibrium of reduced and carboxymethylated enzyme Sus scrofa

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
assay at Sus scrofa

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6
-
assay at Sus scrofa