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Literature summary for 2.4.1.40 extracted from

  • Nagai, M.; Dave, V.; Kaplan, B.E.; Yoshida, A.
    Human blood group glycosyltransferases. I. Purification of N-acetylgalactosaminyltransferase (1987), J. Biol. Chem., 253, 377-379.
    View publication on PubMed
Search for more literature for 2.4.1.40

General Stability

General Stability Organism
enzyme is not stable in crude plasma Homo sapiens

Metals/Ions

Metals/Ions Comment Organism Structure
Mn2+ 15-30 mM required for maximal activity Homo sapiens

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
52000
-
 2 * 52000, SDS-PAGE of carboxymaleyl enzyme Homo sapiens
90000 100000 gel filtration Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
 blood group A1
-

Purification (Commentary)

Purification (Comment) Organism
-
 Homo sapiens

Source Tissue

Source Tissue Comment Organism Textmining
blood plasma blood group A1 Homo sapiens
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
 Homo sapiens

Storage Stability

Storage Stability Organism
-60°C, 2 mM MnCl2, 1 mM EDTA, 0.02 mM UDP, stable for up to 30 days Homo sapiens
4°C, 25% loss of activity after storage of plasma for 2 weeks Homo sapiens
4°C, rapid loss of activity of purified enzyme Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
UDP-N-acetyl-D-galactosamine + 2'-fucosyllactose
-
 Homo sapiens UDP + N-acetyl-alpha-D-galactosaminyl-2'-fucosyllactose
-
 ?
UDP-N-acetyl-D-galactosamine + glycoprotein alpha-L-fucosyl-1,2-D-galactose
-
 Homo sapiens UDP + glycoprotein N-acetyl-alpha-D-galactosaminyl-1,3-[alpha-L-fucosyl-1,2]-D-galactose
-
 ?

Subunits

Subunits Comment Organism
dimer 2 * 52000, SDS-PAGE of carboxymaleyl enzyme Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.5 7
-
 Homo sapiens