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Literature summary for 2.4.1.4 extracted from

  • Lee, H.S.; Kim, T.S.; Parajuli, P.; Pandey, R.P.; Sohng, J.K.
    Sustainable production of dihydroxybenzene glucosides using immobilized amylosucrase from Deinococcus geothermalis (2018), J. Microbiol. Biotechnol., 28, 1447-1456 .
    View publication on PubMed

Application

Application Comment Organism
synthesis immobilization enhances the efficiency of the glycosylation reaction and is therefore considered effective for industrial application in sustainable production of dihydroxybenzene glucosides Deinococcus geothermalis

Cloned(Commentary)

Cloned (Comment) Organism
codon-optimized and expressed in Escherichia coli BL21 (DE3) Deinococcus geothermalis

Organism

Organism UniProt Comment Textmining
Deinococcus geothermalis Q1J0W0
-
-
Deinococcus geothermalis DSM 11300 Q1J0W0
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Deinococcus geothermalis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
sucrose + catechol
-
Deinococcus geothermalis D-fructose + catechol glucoside
-
?
sucrose + catechol
-
Deinococcus geothermalis DSM 11300 D-fructose + catechol glucoside
-
?
sucrose + hydroquinone
-
Deinococcus geothermalis D-fructose + hydroquinone alpha-glucoside
-
?
sucrose + hydroquinone
-
Deinococcus geothermalis DSM 11300 D-fructose + hydroquinone alpha-glucoside
-
?
sucrose + resorcinol
-
Deinococcus geothermalis D-fructose + resorcinol glucoside
-
?
sucrose + resorcinol
-
Deinococcus geothermalis DSM 11300 D-fructose + resorcinol glucoside
-
?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
35
-
substrate: resorcinol or catechol Deinococcus geothermalis
40
-
substrate: hydroquinone Deinococcus geothermalis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
highest yield with hydroquinone (98% conversion) or resorcinol (43.6% conversion) as substrate Deinococcus geothermalis
8
-
highest yield with catechol (60.6% conversion) as substrate Deinococcus geothermalis