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Literature summary for 2.4.1.4 extracted from

  • Zhu, L.; Jiang, D.; Zhou, Y.; Lu, Y.; Fan, Y.; Chen, X.
    Batch-feeding whole-cell catalytic synthesis of alpha-arbutin by amylosucrase from Xanthomonas campestris (2019), J. Ind. Microbiol. Biotechnol., 46, 759-767 .
    View publication on PubMed
Search for more literature for 2.4.1.4

Application

Application Comment Organism
biotechnology arbutin as a safe hydroquinone derivative is one of most important skin-whitening ingredients including beta-arbutin and alpha-arbutin. The batch-feeding whole-cell biocatalysis by Amy-1 is a promising technology for alpha-arbutin production with enhanced yield and molar conversion rate Xanthomonas campestris pv. campestris
synthesis the batch-feeding whole-cell biocatalysis by Amy-1 is a promising technology for alpha-arbutin production with enhanced yield and molar conversion rate Xanthomonas campestris pv. campestris

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Xanthomonas campestris pv. campestris

Inhibitors

Inhibitors Comment Organism Structure
Ba2+ 1.0 mM, 12% inhibition Xanthomonas campestris pv. campestris
Cu2+ 1.0 mM, complete inhibition Xanthomonas campestris pv. campestris
Mn2+ 1.0 mM, 50% inhibition Xanthomonas campestris pv. campestris
Ni2+ 1.0 mM, 30% inhibition Xanthomonas campestris pv. campestris

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
9.58
-
 hydroquinone pH 7.0, 30°C Xanthomonas campestris pv. campestris

Metals/Ions

Metals/Ions Comment Organism Structure
Fe2+ 1.0 mM, 40% activation Xanthomonas campestris pv. campestris
K+ 1.0 mM, 10% activation Xanthomonas campestris pv. campestris

Organism

Organism UniProt Comment Textmining
Xanthomonas campestris pv. campestris A0A0H2X5U3
-
-
Xanthomonas campestris pv. campestris 8004 A0A0H2X5U3
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Xanthomonas campestris pv. campestris

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
sucrose + hydroquinone
-
 Xanthomonas campestris pv. campestris D-fructose + alpha-arbutin
-
 ?
sucrose + hydroquinone
-
 Xanthomonas campestris pv. campestris 8004 D-fructose + alpha-arbutin
-
 ?

Synonyms

Synonyms Comment Organism
Amy-1
-
 Xanthomonas campestris pv. campestris

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
35
-
-
 Xanthomonas campestris pv. campestris

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
20 40 20°C: about 60% of maximal activity, 40°C: about 70% of maximal activity Xanthomonas campestris pv. campestris

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
30
-
 high stability, maintaining 83% activity after 2 h Xanthomonas campestris pv. campestris
50
-
 complete loss of catalytic activity after 30 min Xanthomonas campestris pv. campestris

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
23.9
-
 hydroquinone pH 7.0, 30°C Xanthomonas campestris pv. campestris

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
-
 Xanthomonas campestris pv. campestris

pH Range

pH Minimum pH Maximum Comment Organism
5 8.5 pH 5.0: 65% of maximal activity, pH 8.0: 45% of maximal activity Xanthomonas campestris pv. campestris

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
2.5
-
 hydroquinone pH 7.0, 30°C Xanthomonas campestris pv. campestris