Cloned (Comment) | Organism |
---|---|
gene amsA, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis, recombinant expression of His6-tagged enzyme in Escherichia coli strain BL21(DE3)pLyS | Synechococcus sp. |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Synechococcus sp. | the enzyme AMS exhibits multiple catalytic activities. Primarily, it can hydrolyze sucrose to glucose and fructose or transfer glucose from sucrose hydrolysis to another glucose or acceptormolecule. As a side reaction, it is also able to catalyze the isomerization of sucrose to turanose or trehalulose | ? | - |
? | |
sucrose + [(1->4)-alpha-D-glucosyl]n | Synechococcus sp. | - |
D-fructose + [(1->4)-alpha-D-glucosyl]n+1 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Synechococcus sp. | B1XIU7 | gene amsA | - |
Purification (Comment) | Organism |
---|---|
recombinant His6-tagged enzyme from Escherichia coli strain BL21(DE3)pLyS by cobalt affinity chromatography | Synechococcus sp. |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | the enzyme AMS exhibits multiple catalytic activities. Primarily, it can hydrolyze sucrose to glucose and fructose or transfer glucose from sucrose hydrolysis to another glucose or acceptormolecule. As a side reaction, it is also able to catalyze the isomerization of sucrose to turanose or trehalulose | Synechococcus sp. | ? | - |
? | |
sucrose + [(1->4)-alpha-D-glucosyl]n | - |
Synechococcus sp. | D-fructose + [(1->4)-alpha-D-glucosyl]n+1 | - |
? |
Synonyms | Comment | Organism |
---|---|---|
AMS | - |
Synechococcus sp. |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Synechococcus sp. |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
assay at | Synechococcus sp. |
Organism | Comment | Expression |
---|---|---|
Synechococcus sp. | genes spsA,sppA, frkA and amsA are grouped the Suc cluster, whose expression is increased in response to a salt treatment | up |
General Information | Comment | Organism |
---|---|---|
evolution | the enzyme belongs to the aglycoside-hydrolase family 13. Genes spsA,sppA, frkA and amsA are grouped in a transcriptional unit that is named Suc cluster | Synechococcus sp. |