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Literature summary for 2.4.1.4 extracted from

  • Perez-Cenci, M.; Salerno, G.L.
    Functional characterization of Synechococcus amylosucrase and fructokinase encoding genes discovers two novel actors on the stage of cyanobacterial sucrose metabolism (2014), Plant Sci., 224, 95-102.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
gene amsA, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis, recombinant expression of His6-tagged enzyme in Escherichia coli strain BL21(DE3)pLyS Synechococcus sp.

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Synechococcus sp. the enzyme AMS exhibits multiple catalytic activities. Primarily, it can hydrolyze sucrose to glucose and fructose or transfer glucose from sucrose hydrolysis to another glucose or acceptormolecule. As a side reaction, it is also able to catalyze the isomerization of sucrose to turanose or trehalulose ?
-
?
sucrose + [(1->4)-alpha-D-glucosyl]n Synechococcus sp.
-
D-fructose + [(1->4)-alpha-D-glucosyl]n+1
-
?

Organism

Organism UniProt Comment Textmining
Synechococcus sp. B1XIU7 gene amsA
-

Purification (Commentary)

Purification (Comment) Organism
recombinant His6-tagged enzyme from Escherichia coli strain BL21(DE3)pLyS by cobalt affinity chromatography Synechococcus sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information the enzyme AMS exhibits multiple catalytic activities. Primarily, it can hydrolyze sucrose to glucose and fructose or transfer glucose from sucrose hydrolysis to another glucose or acceptormolecule. As a side reaction, it is also able to catalyze the isomerization of sucrose to turanose or trehalulose Synechococcus sp. ?
-
?
sucrose + [(1->4)-alpha-D-glucosyl]n
-
Synechococcus sp. D-fructose + [(1->4)-alpha-D-glucosyl]n+1
-
?

Synonyms

Synonyms Comment Organism
AMS
-
Synechococcus sp.

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
assay at Synechococcus sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
assay at Synechococcus sp.

Expression

Organism Comment Expression
Synechococcus sp. genes spsA,sppA, frkA and amsA are grouped the Suc cluster, whose expression is increased in response to a salt treatment up

General Information

General Information Comment Organism
evolution the enzyme belongs to the aglycoside-hydrolase family 13. Genes spsA,sppA, frkA and amsA are grouped in a transcriptional unit that is named Suc cluster Synechococcus sp.