BRENDA - Enzyme Database
show all sequences of 2.4.1.4

Biosynthesis of (+)-catechin glycosides using recombinant amylosucrase from Deinococcus geothermalis DSM 11300

Cho, H.K.; Kim, H.H.; Seo, D.H.; Jung, J.H.; Park, J.H.; Baek, N.I.; Kim, M.J.; Yoo, S.H.; Cha, J.; Kim, Y.R.; Park, C.S.; Enzyme Microb. Technol. 49, 246-253 (2011)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
gene dgas, expression of GST-tagged DGAS in Escherichia coli
Deinococcus geothermalis
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
sucrose + [(1->4)-alpha-D-glucosyl]n
Deinococcus geothermalis
-
D-fructose + [(1->4)-alpha-D-glucosyl]n+1
-
-
?
sucrose + [(1->4)-alpha-D-glucosyl]n
Deinococcus geothermalis DSM 11300
-
D-fructose + [(1->4)-alpha-D-glucosyl]n+1
-
-
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Deinococcus geothermalis
Q1J0W0
gene dgas
-
Deinococcus geothermalis DSM 11300
Q1J0W0
gene dgas
-
Purification (Commentary)
Commentary
Organism
recombinant GST-tagged DGAS from Escherichia coli by glutathione affinity chromatography
Deinococcus geothermalis
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
additional information
NMR product analysis, overview
719376
Deinococcus geothermalis
?
-
-
-
-
additional information
NMR product analysis, overview
719376
Deinococcus geothermalis DSM 11300
?
-
-
-
-
sucrose + (+)-catechin
-
719376
Deinococcus geothermalis
D-fructose + (+)-catechin-3'-O-alpha-D-glucopyranoside
-
-
-
?
sucrose + (+)-catechin
-
719376
Deinococcus geothermalis DSM 11300
D-fructose + (+)-catechin-3'-O-alpha-D-glucopyranoside
-
-
-
?
sucrose + maltose
the enzyme also produces (+)-catechin maltooligosaccharides
719376
Deinococcus geothermalis
D-fructose + (+)-catechin-3'-O-alpha-D-maltoside
-
-
-
?
sucrose + maltose
the enzyme also produces (+)-catechin maltooligosaccharides
719376
Deinococcus geothermalis DSM 11300
D-fructose + (+)-catechin-3'-O-alpha-D-maltoside
-
-
-
?
sucrose + [(1->4)-alpha-D-glucosyl]n
-
719376
Deinococcus geothermalis
D-fructose + [(1->4)-alpha-D-glucosyl]n+1
-
-
-
?
sucrose + [(1->4)-alpha-D-glucosyl]n
a phenolic aglycone compound can also act an acceptor molecule of ASase transglycosylation activity
719376
Deinococcus geothermalis
D-fructose + [(1->4)-alpha-D-glucosyl]n+1
-
-
-
?
sucrose + [(1->4)-alpha-D-glucosyl]n
-
719376
Deinococcus geothermalis DSM 11300
D-fructose + [(1->4)-alpha-D-glucosyl]n+1
-
-
-
?
sucrose + [(1->4)-alpha-D-glucosyl]n
a phenolic aglycone compound can also act an acceptor molecule of ASase transglycosylation activity
719376
Deinococcus geothermalis DSM 11300
D-fructose + [(1->4)-alpha-D-glucosyl]n+1
-
-
-
?
Temperature Optimum [°C]
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
45
-
assay at
Deinococcus geothermalis
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7
-
assay at
Deinococcus geothermalis
Cloned(Commentary) (protein specific)
Commentary
Organism
gene dgas, expression of GST-tagged DGAS in Escherichia coli
Deinococcus geothermalis
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
sucrose + [(1->4)-alpha-D-glucosyl]n
Deinococcus geothermalis
-
D-fructose + [(1->4)-alpha-D-glucosyl]n+1
-
-
?
sucrose + [(1->4)-alpha-D-glucosyl]n
Deinococcus geothermalis DSM 11300
-
D-fructose + [(1->4)-alpha-D-glucosyl]n+1
-
-
?
Purification (Commentary) (protein specific)
Commentary
Organism
recombinant GST-tagged DGAS from Escherichia coli by glutathione affinity chromatography
Deinococcus geothermalis
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
additional information
NMR product analysis, overview
719376
Deinococcus geothermalis
?
-
-
-
-
additional information
NMR product analysis, overview
719376
Deinococcus geothermalis DSM 11300
?
-
-
-
-
sucrose + (+)-catechin
-
719376
Deinococcus geothermalis
D-fructose + (+)-catechin-3'-O-alpha-D-glucopyranoside
-
-
-
?
sucrose + (+)-catechin
-
719376
Deinococcus geothermalis DSM 11300
D-fructose + (+)-catechin-3'-O-alpha-D-glucopyranoside
-
-
-
?
sucrose + maltose
the enzyme also produces (+)-catechin maltooligosaccharides
719376
Deinococcus geothermalis
D-fructose + (+)-catechin-3'-O-alpha-D-maltoside
-
-
-
?
sucrose + maltose
the enzyme also produces (+)-catechin maltooligosaccharides
719376
Deinococcus geothermalis DSM 11300
D-fructose + (+)-catechin-3'-O-alpha-D-maltoside
-
-
-
?
sucrose + [(1->4)-alpha-D-glucosyl]n
-
719376
Deinococcus geothermalis
D-fructose + [(1->4)-alpha-D-glucosyl]n+1
-
-
-
?
sucrose + [(1->4)-alpha-D-glucosyl]n
a phenolic aglycone compound can also act an acceptor molecule of ASase transglycosylation activity
719376
Deinococcus geothermalis
D-fructose + [(1->4)-alpha-D-glucosyl]n+1
-
-
-
?
sucrose + [(1->4)-alpha-D-glucosyl]n
-
719376
Deinococcus geothermalis DSM 11300
D-fructose + [(1->4)-alpha-D-glucosyl]n+1
-
-
-
?
sucrose + [(1->4)-alpha-D-glucosyl]n
a phenolic aglycone compound can also act an acceptor molecule of ASase transglycosylation activity
719376
Deinococcus geothermalis DSM 11300
D-fructose + [(1->4)-alpha-D-glucosyl]n+1
-
-
-
?
Temperature Optimum [°C] (protein specific)
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
45
-
assay at
Deinococcus geothermalis
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7
-
assay at
Deinococcus geothermalis
Other publictions for EC 2.4.1.4
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
736029
Seo
An unusual chimeric amylosucra ...
Deinococcus geothermalis, Neisseria polysaccharea
Enzyme Microb. Technol.
86
7-16
2016
-
-
2
-
2
-
-
-
-
-
-
2
-
5
-
-
2
-
-
-
-
-
2
2
4
-
2
-
4
2
-
-
-
-
-
-
-
2
-
-
2
-
-
-
-
-
-
-
-
2
-
-
-
2
-
-
-
-
2
2
4
-
2
-
4
2
-
-
-
-
-
-
-
-
735570
But
Sucrose metabolism in halotole ...
Methylomicrobium alcaliphilum 20Z, Methylomicrobium alcaliphilum
Arch. Microbiol.
197
471-480
2015
-
-
1
-
-
-
-
1
-
-
1
2
-
6
-
-
-
-
-
-
-
-
2
1
1
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
1
-
-
1
2
-
-
-
-
-
-
-
-
2
1
1
-
-
-
1
-
-
-
-
-
-
-
-
-
735887
Kim
Characterization of enzymatica ...
Neisseria polysaccharea
Carbohydr. Polym.
125
61-68
2015
-
1
-
-
-
-
-
-
-
-
-
1
-
4
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
736543
Overwin
Biotransformation of phloretin ...
Neisseria polysaccharea, Neisseria polysaccharea ATCC 43768
J. Biotechnol.
211
103-106
2015
-
1
-
-
-
-
-
-
-
-
-
2
-
3
-
-
-
-
-
-
-
-
4
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
4
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
735462
Jeong
Biosynthesis of glucosyl glyce ...
Methylobacillus flagellatus, Methylobacillus flagellatus ATCC 51484
Appl. Biochem. Biotechnol.
173
904-917
2014
-
-
1
-
-
-
-
-
-
-
1
-
-
4
-
-
1
-
-
-
-
-
6
-
1
1
1
-
1
1
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
1
-
-
-
-
6
-
1
1
1
-
1
1
-
-
-
1
1
-
-
-
735805
Cambon
Essential role of amino acid p ...
Neisseria polysaccharea
Biotechnol. Bioeng.
111
1719-1728
2014
-
-
1
-
3
-
-
8
-
-
-
5
-
2
-
-
-
-
-
-
-
-
5
1
1
-
-
16
1
-
-
-
-
-
-
-
-
1
-
-
3
-
-
-
-
8
-
-
-
5
-
-
-
-
-
-
-
-
5
1
1
-
-
16
1
-
-
-
-
2
2
-
8
8
736134
Kim
Branch chain elongation by amy ...
Neisseria polysaccharea
Food Chem.
152
113-120
2014
-
-
-
-
-
-
-
-
-
-
-
1
-
2
-
-
-
-
-
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2
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1
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-
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1
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-
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-
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-
-
-
-
-
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-
1
-
-
-
-
-
-
-
-
2
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
736140
Kim
-
Molecular cloning and expressi ...
Deinococcus radiopugnans, Deinococcus radiopugnans ATCC 19172
Food Sci. Biotechnol.
23
2007-2012
2014
-
-
1
-
-
-
-
-
-
-
3
2
-
2
-
-
1
-
-
-
-
-
4
1
1
1
1
-
1
-
-
-
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
-
3
2
-
-
-
1
-
-
-
-
4
1
1
1
1
-
1
-
-
1
-
2
2
-
-
-
737043
Perez-Cenci
Functional characterization of ...
Synechococcus sp.
Plant Sci.
224
95-102
2014
-
-
1
-
-
-
-
-
-
-
-
2
-
6
-
-
1
-
-
-
-
-
2
-
1
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
1
-
-
-
-
2
-
1
-
-
-
1
-
-
-
1
1
1
1
-
-
737050
Huang
Expression of an amylosucrase ...
Neisseria polysaccharea
Planta
240
409-421
2014
-
1
1
-
1
-
-
-
-
-
2
-
-
3
-
-
-
-
-
-
-
-
-
1
-
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-
-
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-
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1
1
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-
1
-
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2
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-
-
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-
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1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
735389
Skov
The structure of amylosucrase ...
Deinococcus radiodurans
Acta Crystallogr. Sect. F
69
973-978
2013
-
-
1
1
-
-
-
-
-
-
-
1
-
4
-
-
1
1
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1
1
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1
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1
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1
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1
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1
1
-
-
-
-
-
-
-
-
-
1
1
-
-
-
735955
Daude
-
Probing substrate promiscuity ...
Neisseria polysaccharea
ChemCatChem
5
2288-2295
2013
-
-
-
-
-
-
-
-
-
-
-
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1
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1
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1
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1
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1
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
737182
Liu
-
Insights into the working mech ...
Pseudarthrobacter chlorophenolicus
Prog. Biochem. Biophys.
40
565-577
2013
-
-
-
-
-
1
-
-
-
-
-
1
-
1
-
-
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-
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-
2
1
-
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1
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1
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-
2
1
-
-
-
-
-
-
-
-
-
1
1
-
-
-
737204
Daude
Probing impact of active site ...
Neisseria polysaccharea
Protein Sci.
22
1754-1765
2013
-
-
1
-
24
-
-
-
-
-
-
1
-
2
-
-
-
-
-
-
-
-
2
1
1
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1
-
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1
-
-
24
-
-
-
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1
-
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2
1
1
-
-
-
1
-
-
-
-
2
2
-
-
-
718673
Seo
High-yield enzymatic bioconver ...
Deinococcus geothermalis
Appl. Microbiol. Biotechnol.
94
1189-1197
2012
1
-
1
-
-
-
-
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-
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1
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3
-
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1
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1
1
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1
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1
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1
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1
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1
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1
1
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1
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-
-
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720052
Guerin
Structural investigation of th ...
Deinococcus geothermalis, Neisseria polysaccharea
J. Biol. Chem.
287
6642-6654
2012
-
-
2
2
1
-
-
-
-
-
-
2
-
5
-
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2
2
-
-
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-
4
2
2
-
2
-
2
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-
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2
-
2
1
-
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-
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2
-
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-
2
-
-
-
-
4
2
2
-
2
-
2
-
-
-
-
6
6
-
-
-
720211
Seo
Functional expression of amylo ...
Pseudarthrobacter chlorophenolicus, Pseudarthrobacter chlorophenolicus A6 / DSM 12829
J. Microbiol. Biotechnol.
22
1253-1257
2012
-
-
1
-
-
-
-
-
-
-
2
-
-
5
-
-
1
-
-
-
-
-
-
1
1
1
1
-
1
1
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
1
-
-
-
-
-
1
1
1
1
-
1
1
-
-
-
1
1
-
-
-
720870
Liu
Insight into the structure, dy ...
Deinococcus geothermalis
PLoS ONE
7
e40441
2012
-
-
-
-
-
-
-
-
-
-
-
-
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3
-
-
-
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1
-
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-
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1
-
-
-
-
-
-
-
-
-
-
2
2
-
-
-
719106
Wang
Preparation and characterizati ...
Neisseria polysaccharea, Neisseria polysaccharea ATCC 43768
Biores. Technol.
102
6370-6374
2011
-
-
-
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1
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-
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3
-
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MacKenzie
Glycogen metabolism in the gen ...
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Glycogen synthesis by amylosuc ...
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New studies on amylosucrase, a ...
Neisseria perflava
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1
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489139
Okada
De novo synthesis of glycosidi ...
Neisseria perflava
Carbohydr. Res.
26
240-243
1973
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489134
Feingold
Enzymatic synthesis and reacti ...
Neisseria perflava
J. Biol. Chem.
224
295-307
1957
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