Application | Comment | Organism |
---|---|---|
synthesis | mutant enzyme R226A, that is activated by the products it forms and yields twice as much insoluble glucan and lower quantities of by-products as the wild-type enzyme is a very promising enzyme for industrial synthesis of amylose-like polymers | Neisseria polysaccharea |
Protein Variants | Comment | Organism |
---|---|---|
D394A | 23.5% of the wild-type activity, according to the initial rate of sucrose consumption, very poor ativation by glycogen | Neisseria polysaccharea |
R226A | activated by the products it forms. The mutant yields twice as much insoluble glucan as the wild-type enzyme and leads to the production of lower quantities of by-products, mutant enzyme is strongly activated by glycogen | Neisseria polysaccharea |
R415A | 4.3% of the activity compared with the wild-type enzyme. No synthesis of any insoluble modified glycogen | Neisseria polysaccharea |
R446A | 15% of the wild-type activity, according to the initial rate of sucrose consumption, no synthesis of any insoluble modified glycogen | Neisseria polysaccharea |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
sucrose + (1,4-alpha-D-glucosyl)n | Neisseria polysaccharea | amylosucrase initializes polymer formation by releasing, through sucrose hydrolysis, a glucose molecule that is subsequently used as the first acceptor molecule. Maltooligosaccharides of increasing size are produced and successively elongated at their nonreducing ends until they reached a critical size and concentration, causing precipitation | D-fructose + (1,4-alpha-D-glucosyl)n+1 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Neisseria polysaccharea | - |
- |
- |
Purification (Comment) | Organism |
---|---|
wild-type and mutant enzymes | Neisseria polysaccharea |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
sucrose | - |
Neisseria polysaccharea | ? | - |
? | |
sucrose + (1,4-alpha-D-glucosyl)n | amylosucrase initializes polymer formation by releasing, through sucrose hydrolysis, a glucose molecule that is subsequently used as the first acceptor molecule. Maltooligosaccharides of increasing size are produced and successively elongated at their nonreducing ends until they reached a critical size and concentration, causing precipitation | Neisseria polysaccharea | D-fructose + (1,4-alpha-D-glucosyl)n+1 | - |
? | |
sucrose + glycogen | - |
Neisseria polysaccharea | ? | - |
? | |
sucrose + maltoheptaose | - |
Neisseria polysaccharea | ? | - |
? |