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Literature summary for 2.4.1.38 extracted from

  • Saliba, M.; Ramalanjaona, N.; Gulberti, S.; Bertin-Jung, I.; Thomas, A.; Dahbi, S.; Lopin-Bon, C.; Jacquinet, J.C.; Breton, C.; Ouzzine, M.; Fournel-Gigleux, S.
    Probing the acceptor active site organization of the human recombinant beta1,4-galactosyltransferase 7 and design of xyloside-based inhibitors (2015), J. Biol. Chem., 290, 7658-7670.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.4.1.38

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Homo sapiens

Protein Variants

Protein Variants Comment Organism
H195A the mutant shows reduced activity compared to the wild type enzyme Homo sapiens
H195Q the mutant shows reduced activity compared to the wild type enzyme Homo sapiens
H195R the mutant shows reduced activity compared to the wild type enzyme Homo sapiens
R226A the mutant shows reduced activity compared to the wild type enzyme Homo sapiens
R226K the mutant shows reduced activity compared to the wild type enzyme Homo sapiens
R270A the mutant shows reduced activity compared to the wild type enzyme Homo sapiens
R270K the mutant shows reduced activity compared to the wild type enzyme Homo sapiens
Y194A inactive Homo sapiens
Y194F inactive Homo sapiens
Y196A inactive Homo sapiens
Y196F the mutant shows reduced activity compared to the wild type enzyme Homo sapiens
Y199A inactive Homo sapiens
Y199F the mutant shows reduced activity compared to the wild type enzyme Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
4-methylumbelliferyl 4-deoxy-4-fluoro-beta-D-xylopyranoside
-
 Homo sapiens
4-methylumbelliferyl 4-deoxy-beta-D-xylopyranoside
-
 Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.22
-
 UDP-alpha-D-galactose recombinant wild type enzyme, at pH 7.0 and 37°C Homo sapiens
0.27
-
 UDP-alpha-D-galactose mutant enzyme R270A, at pH 7.0 and 37°C Homo sapiens
0.29
-
 UDP-alpha-D-galactose mutant enzyme R226K, at pH 7.0 and 37°C Homo sapiens
0.32
-
 UDP-alpha-D-galactose mutant enzyme H195R, at pH 7.0 and 37°C Homo sapiens
0.32
-
 UDP-alpha-D-galactose mutant enzyme Y199F, at pH 7.0 and 37°C Homo sapiens
0.33
-
 UDP-alpha-D-galactose mutant enzyme H195Q, at pH 7.0 and 37°C Homo sapiens
0.34
-
 UDP-alpha-D-galactose mutant enzyme R226A, at pH 7.0 and 37°C Homo sapiens
0.34
-
 UDP-alpha-D-galactose mutant enzyme Y196F, at pH 7.0 and 37°C Homo sapiens
0.35
-
 4-methylumbelliferyl-beta-D-xylopyranoside mutant enzyme H195R, at pH 7.0 and 37°C Homo sapiens
0.35
-
 4-methylumbelliferyl-beta-D-xylopyranoside recombinant wild type enzyme, at pH 7.0 and 37°C Homo sapiens
0.37
-
 UDP-alpha-D-galactose mutant enzyme R270K, at pH 7.0 and 37°C Homo sapiens
0.4
-
 UDP-alpha-D-galactose mutant enzyme H195A, at pH 7.0 and 37°C Homo sapiens
0.44
-
 4-methylumbelliferyl-beta-D-xylopyranoside mutant enzyme R226A, at pH 7.0 and 37°C Homo sapiens
0.46
-
 4-methylumbelliferyl-beta-D-xylopyranoside mutant enzyme R226K, at pH 7.0 and 37°C Homo sapiens
0.54
-
 4-methylumbelliferyl-beta-D-xylopyranoside mutant enzyme R270K, at pH 7.0 and 37°C Homo sapiens
0.55
-
 4-methylumbelliferyl-beta-D-xylopyranoside mutant enzyme H195Q, at pH 7.0 and 37°C Homo sapiens
0.59
-
 4-methylumbelliferyl-beta-D-xylopyranoside mutant enzyme Y199F, at pH 7.0 and 37°C Homo sapiens
0.6
-
 4-methylumbelliferyl-beta-D-xylopyranoside mutant enzyme R270A, at pH 7.0 and 37°C Homo sapiens
0.64
-
 4-methylumbelliferyl-beta-D-xylopyranoside mutant enzyme H195A, at pH 7.0 and 37°C Homo sapiens
1.06
-
 4-methylumbelliferyl-beta-D-xylopyranoside mutant enzyme Y196F, at pH 7.0 and 37°C Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens Q9UBV7
-
-

Purification (Commentary)

Purification (Comment) Organism
glutathione Sepharose column chromatography Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
UDP-alpha-D-galactose + 4-methylumbelliferyl-beta-D-xylopyranoside
-
 Homo sapiens ?
-
 ?

Synonyms

Synonyms Comment Organism
beta1,4-galactosyltransferase 7
-
 Homo sapiens
beta4GalT7
-
 Homo sapiens

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.5
-
 UDP-alpha-D-galactose mutant enzyme Y196F, at pH 7.0 and 37°C Homo sapiens
0.5
-
 4-methylumbelliferyl-beta-D-xylopyranoside mutant enzyme Y196F, at pH 7.0 and 37°C Homo sapiens
0.77
-
 UDP-alpha-D-galactose mutant enzyme R270A, at pH 7.0 and 37°C Homo sapiens
0.77
-
 4-methylumbelliferyl-beta-D-xylopyranoside mutant enzyme R270A, at pH 7.0 and 37°C Homo sapiens
0.82
-
 UDP-alpha-D-galactose mutant enzyme R270K, at pH 7.0 and 37°C Homo sapiens
0.82
-
 4-methylumbelliferyl-beta-D-xylopyranoside mutant enzyme R270K, at pH 7.0 and 37°C Homo sapiens
0.89
-
 UDP-alpha-D-galactose mutant enzyme R226A, at pH 7.0 and 37°C Homo sapiens
0.89
-
 4-methylumbelliferyl-beta-D-xylopyranoside mutant enzyme R226A, at pH 7.0 and 37°C Homo sapiens
1.2
-
 UDP-alpha-D-galactose mutant enzyme Y199F, at pH 7.0 and 37°C Homo sapiens
1.2
-
 4-methylumbelliferyl-beta-D-xylopyranoside mutant enzyme Y199F, at pH 7.0 and 37°C Homo sapiens
1.35
-
 UDP-alpha-D-galactose mutant enzyme R226K, at pH 7.0 and 37°C Homo sapiens
1.35
-
 4-methylumbelliferyl-beta-D-xylopyranoside mutant enzyme R226K, at pH 7.0 and 37°C Homo sapiens
1.49
-
 UDP-alpha-D-galactose mutant enzyme H195R, at pH 7.0 and 37°C Homo sapiens
1.49
-
 4-methylumbelliferyl-beta-D-xylopyranoside mutant enzyme H195R, at pH 7.0 and 37°C Homo sapiens
1.51
-
 UDP-alpha-D-galactose recombinant wild type enzyme, at pH 7.0 and 37°C Homo sapiens
1.51
-
 4-methylumbelliferyl-beta-D-xylopyranoside recombinant wild type enzyme, at pH 7.0 and 37°C Homo sapiens
1.62
-
 UDP-alpha-D-galactose mutant enzyme H195Q, at pH 7.0 and 37°C Homo sapiens
1.62
-
 4-methylumbelliferyl-beta-D-xylopyranoside mutant enzyme H195Q, at pH 7.0 and 37°C Homo sapiens
1.93
-
 UDP-alpha-D-galactose mutant enzyme H195A, at pH 7.0 and 37°C Homo sapiens
1.93
-
 4-methylumbelliferyl-beta-D-xylopyranoside mutant enzyme H195A, at pH 7.0 and 37°C Homo sapiens

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.06
-
 4-methylumbelliferyl 4-deoxy-4-fluoro-beta-D-xylopyranoside recombinant wild type enzyme, at pH 7.0 and 37°C Homo sapiens
1.28
-
 4-methylumbelliferyl 4-deoxy-beta-D-xylopyranoside recombinant wild type enzyme, at pH 7.0 and 37°C Homo sapiens

IC50 Value

IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
0.03
-
 recombinant wild type enzyme, at pH 7.0 and 37°C Homo sapiens 4-methylumbelliferyl 4-deoxy-4-fluoro-beta-D-xylopyranoside
0.53
-
 recombinant wild type enzyme, at pH 7.0 and 37°C Homo sapiens 4-methylumbelliferyl 4-deoxy-beta-D-xylopyranoside

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.47
-
 4-methylumbelliferyl-beta-D-xylopyranoside mutant enzyme Y196F, at pH 7.0 and 37°C Homo sapiens
1.2
-
 4-methylumbelliferyl-beta-D-xylopyranoside mutant enzyme R270A, at pH 7.0 and 37°C Homo sapiens
1.42
-
 4-methylumbelliferyl-beta-D-xylopyranoside mutant enzyme R270K, at pH 7.0 and 37°C Homo sapiens
1.47
-
 UDP-alpha-D-galactose mutant enzyme Y196F, at pH 7.0 and 37°C Homo sapiens
1.87
-
 4-methylumbelliferyl-beta-D-xylopyranoside mutant enzyme R226A, at pH 7.0 and 37°C Homo sapiens
1.88
-
 4-methylumbelliferyl-beta-D-xylopyranoside mutant enzyme Y199F, at pH 7.0 and 37°C Homo sapiens
2.32
-
 UDP-alpha-D-galactose mutant enzyme R270K, at pH 7.0 and 37°C Homo sapiens
2.85
-
 UDP-alpha-D-galactose mutant enzyme R226A, at pH 7.0 and 37°C Homo sapiens
2.92
-
 4-methylumbelliferyl-beta-D-xylopyranoside mutant enzyme R226K, at pH 7.0 and 37°C Homo sapiens
2.95
-
 4-methylumbelliferyl-beta-D-xylopyranoside mutant enzyme H195Q, at pH 7.0 and 37°C Homo sapiens
3
-
 4-methylumbelliferyl-beta-D-xylopyranoside mutant enzyme H195A, at pH 7.0 and 37°C Homo sapiens
3.07
-
 UDP-alpha-D-galactose mutant enzyme R270A, at pH 7.0 and 37°C Homo sapiens
4.03
-
 4-methylumbelliferyl-beta-D-xylopyranoside mutant enzyme H195R, at pH 7.0 and 37°C Homo sapiens
4.05
-
 UDP-alpha-D-galactose mutant enzyme Y199F, at pH 7.0 and 37°C Homo sapiens
4.17
-
 4-methylumbelliferyl-beta-D-xylopyranoside recombinant wild type enzyme, at pH 7.0 and 37°C Homo sapiens
4.7
-
 UDP-alpha-D-galactose mutant enzyme R226K, at pH 7.0 and 37°C Homo sapiens
4.85
-
 UDP-alpha-D-galactose mutant enzyme H195A, at pH 7.0 and 37°C Homo sapiens
4.92
-
 UDP-alpha-D-galactose mutant enzyme H195Q, at pH 7.0 and 37°C Homo sapiens
4.92
-
 UDP-alpha-D-galactose mutant enzyme H195R, at pH 7.0 and 37°C Homo sapiens
7.08
-
 UDP-alpha-D-galactose recombinant wild type enzyme, at pH 7.0 and 37°C Homo sapiens